發(fā)布時(shí)間：2018-06-23 03:45

  本文選題：細(xì)菌性腦膜炎 + 腦脊液　； 參考：《中南大學(xué)》2013年碩士論文

【摘要】：目的： 在兔實(shí)驗(yàn)性大腸埃希菌腦膜炎中觀察兔一般情況、腦脊液改變,初步探討細(xì)菌性腦膜炎時(shí)是否存在脈絡(luò)叢組織上GLUT1蛋白受損,是否與腦脊液糖/血糖比值的變化有關(guān)。 方法： 1.驗(yàn)證兔脈絡(luò)叢組織GLUT1的表達(dá) 2.制備兔實(shí)驗(yàn)性大腸埃希茵腦膜炎模型。 3.觀察家兔一般情況,小腦延髓池穿刺取腦脊液進(jìn)行相關(guān)檢測(cè),并對(duì)各指標(biāo)進(jìn)行相關(guān)性分析。 4.采用Real-time PCR和West-blot檢測(cè)腦脈絡(luò)叢組織GLUT1基因mRNA水平和蛋白水平的表達(dá)。 結(jié)果： 1.本次實(shí)驗(yàn)驗(yàn)證了脈絡(luò)叢組織中存在GLUT1的表達(dá) 2.本研究成功制備了家兔大腸埃希菌腦膜炎動(dòng)物模型。 3.與注菌前及對(duì)照組比較：模型組家兔體溫升高(P0.05),同時(shí)伴隨精神、進(jìn)食、活動(dòng)等改變；腦脊液外觀渾濁,白細(xì)胞數(shù)、蛋白含量明顯升高(P0.01),腦脊液乳酸升高(P0.01),腦脊液糖、腦脊液糖/血糖比值明顯下降(P0.01),差異均有統(tǒng)計(jì)學(xué)意義,腦脊液白細(xì)胞數(shù)、蛋白與腦脊液乳酸、腦脊液糖及腦脊液糖/血糖比值各指標(biāo)間比較有相關(guān)性。 4.模型組的脈絡(luò)叢組織GLUT1mRNA和蛋白相對(duì)表達(dá)量減少(P0.05)。 結(jié)論： 1兔實(shí)驗(yàn)性大腸埃希菌腦膜炎時(shí)腦脊液乳酸顯著升高,腦脊液糖含量及腦脊液糖/血糖比值有明顯降低,且腦脊液糖/血糖比值小于0.5,且以上各項(xiàng)指標(biāo)與腦脊液白細(xì)胞數(shù)、腦脊液蛋白具有相關(guān)性。 2.本研究首次提出細(xì)菌性腦膜炎時(shí)脈絡(luò)叢組織上GLUT1蛋白受損,可能與腦脊液糖/血糖比值下降有關(guān)。
[Abstract]:Objective: to observe the general situation and changes of cerebrospinal fluid (CSF) in rabbits with experimental Escherichia coli meningitis, and to explore whether GLUT1 protein was damaged in choroid plexus tissue during bacterial meningitis. Whether or not it is related to the change of CSF sugar / glucose ratio. Methods: 1. To verify the expression of GLUT1 in choroidal plexus of rabbits. A rabbit model of experimental Escherichia coli meningitis was established. Observe the general situation of rabbits, cerebellar medullary cistern puncture to take cerebrospinal fluid for correlation detection, and the correlation analysis of each index. 4. 4. Real-time PCR and West-blot were used to detect the expression of GLUT1 mRNA and protein in choroid plexus. Results: 1. This experiment confirmed the existence of GLUT1 expression in choroidal plexus. The animal model of Escherichia coli meningitis in rabbits was established successfully. Compared with the control group and before the injection of bacteria, the temperature of the model group increased (P0.05), accompanied by mental, eating, activity and other changes, cerebrospinal fluid appearance turbidity, white blood cell count, protein content significantly increased (P0.01), cerebrospinal fluid lactic acid (P0.01), cerebrospinal fluid sugar, and so on, The ratio of CSF sugar / glucose decreased significantly (P0.01). The number of CSF leukocytes and protein were correlated with cerebrospinal fluid lactic acid, CSF sugar and CSF sugar / glucose ratio. 4. The relative expression of GLUT1 mRNA and protein in choroid plexus was decreased in model group (P0.05). Conclusion: 1 the level of cerebrospinal fluid (CSF) lactic acid and the ratio of cerebrospinal fluid sugar (CSF) to glucose (CSF) were significantly decreased in rabbits with experimental Escherichia coli meningitis. The ratio of CSF sugar to glucose was less than 0.5, and the above indexes were correlated with the number of CSF leukocytes and cerebrospinal fluid (CSF) protein. 2. This study suggested for the first time that GLUT1 protein in choroid plexus was damaged in bacterial meningitis, which may be related to the decrease of CSF glucose / glucose ratio.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R725.1

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