本文選題：嬰幼兒血管瘤 + 心得安�。� 參考：《華中科技大學(xué)》2013年碩士論文

【摘要】：目的：通過免疫熒光技術(shù)檢測β2受體在嬰幼兒血管瘤組織中的表達(dá)及分布特點,探討其在血管瘤組織病理演變過程中的作用及意義 方法：將48例嬰幼兒血管瘤進(jìn)行HE染色，，根據(jù)臨床表現(xiàn)和組織學(xué)特征，分為增殖期嬰幼兒血管瘤組和消退期嬰幼兒血管瘤組，其中增殖期嬰幼兒血管瘤29例，消退期嬰幼兒血管瘤19例。采用免疫熒光技術(shù)分別將兩組嬰幼兒血管瘤組織中內(nèi)皮細(xì)胞核及β2受體進(jìn)行熒光標(biāo)記，觀察兩組照片熒光標(biāo)記物的顯示位置，并應(yīng)用Image-ProPlus6.0軟件先將熒光照片轉(zhuǎn)換為黑白圖片然后對每張照片進(jìn)行分析,得出內(nèi)皮細(xì)胞核及β2受體陽性的平均光密度值。 結(jié)果：增殖期嬰幼兒血管瘤組內(nèi)皮細(xì)胞的平均光密度值為0.031±0.002，消退期嬰幼兒血管瘤組β2受體的平均光密度值均數(shù)為0.022±0.002，兩者具有顯著統(tǒng)計學(xué)差異。增殖期嬰幼兒血管瘤組β2受體的平均光密度值為0.034±0.003，消退期嬰幼兒血管瘤組β2受體的平均光密度值均數(shù)為0.027±0.002，兩者具有顯著統(tǒng)計學(xué)差異。增殖期嬰幼兒血管瘤中β2受體的表達(dá)明顯高于消退期嬰幼兒血管瘤。熒光圖片顯示β2受體于嬰幼兒血管瘤中廣泛表達(dá)，并以內(nèi)皮細(xì)胞核熒光表達(dá)處為主。 結(jié)論：增殖期嬰幼兒血管瘤中內(nèi)皮細(xì)胞及β2受體的表達(dá)均明顯高于消退期嬰幼兒血管瘤，消退期嬰幼兒血管瘤組織中內(nèi)皮細(xì)胞核的消退伴隨β2受體表達(dá)的減弱，可推測β2受體主要廣泛表達(dá)于嬰幼兒血管瘤內(nèi)皮細(xì)胞中，且可能存在于周細(xì)胞中。提示β2受體阻滯劑劑作用于嬰幼兒血管瘤，誘導(dǎo)其消退的機(jī)制可能與內(nèi)皮細(xì)胞及周細(xì)胞中β2受體有關(guān)。
[Abstract]:Objective: to detect the expression and distribution of 尾 2 receptor in infantile hemangioma by immunofluorescence technique. Methods: 48 cases of infantile hemangioma were stained with HE according to their clinical manifestations and histological features. There were 29 cases of proliferative hemangioma and 19 cases of involutive hemangioma in infantile hemangioma. The endothelial nuclei and 尾 2 receptors were labeled with immunofluorescence technique in two groups of infantile hemangioma tissues, and the display position of the fluorescent markers in the two groups were observed. Using Image-ProPlus6.0 software, the fluorescence photos were converted into black and white pictures, and then each photo was analyzed, and the average optical density of endothelial cell nucleus and 尾 2 receptor positive was obtained. Results: the average optical density of endothelial cells was 0.031 鹵0.002 in proliferative hemangioma group and 0.022 鹵0.002 in involutive phase. The average optical density of 尾 2 receptor was 0.034 鹵0.003 in proliferative hemangioma group and 0.027 鹵0.002 in involutive phase. There was significant difference between the two groups. The expression of 尾 2 receptor in proliferative hemangioma was significantly higher than that in involutive hemangioma. Fluorescence images showed that 尾 2 receptor was widely expressed in infantile hemangioma, and mainly in endothelial cell nucleus. Conclusion: the expression of endothelial cells and 尾 2 receptor in proliferative hemangioma is significantly higher than that in involutive hemangioma. It can be inferred that 尾 2 receptor is mainly expressed in endothelial cells of infantile hemangioma and may exist in pericytes. It is suggested that the mechanism of 尾 2 receptor antagonist acting on infantile hemangioma may be related to 尾 2 receptor in endothelial cells and pericytes.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R732.2

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