本文選題：低出生體重兒 + 蛋白質(zhì)組學(xué)�。� 參考：《瀘州醫(yī)學(xué)院》2012年碩士論文

【摘要】：目的：低出生體重新生兒（Low birth weight infant, LBW）是指出生1小時(shí)內(nèi)體重不足2500g者。一般是早產(chǎn)兒或小于胎齡兒，小于齡兒（small for gestational age, SGA）又稱胎兒宮內(nèi)生長遲緩（intrauterinegrowth retardation, IUGR）。本實(shí)驗(yàn)應(yīng)用表面增強(qiáng)激光解吸電離飛行時(shí)間質(zhì)(surface enhanced laser desorption/ionization-time of flight-massspectrometry, SELDI-TOF-MS)技術(shù)研究LBW營養(yǎng)狀況，以期為LBW的臨床治療提供參考依據(jù)，盡快啟動(dòng)更為合理的治療方案，改善其預(yù)后。方法：將采集的91份血清樣本分為4組，其中LBW59例：早產(chǎn)低出生體重兒（PLBW）37例，足月低出生體重兒（MLBW）22例；正常出生體重新生兒（NBW）32例。采用表面增強(qiáng)激光解析電離飛行時(shí)間質(zhì)譜（SELDI-TOF-MS）技術(shù)及金（Au）芯片采集各組血清蛋白質(zhì)譜指紋圖，并用Biomarker Wizard軟件篩選出差異蛋白。將所檢測(cè)到有顯著差異蛋白質(zhì)峰質(zhì)荷比（M/Z）輸入蛋白質(zhì)數(shù)據(jù)庫(Swiss蛋白數(shù)據(jù)庫http//us.expasy.org/tools/tagident.html)，得到與這些蛋白質(zhì)峰質(zhì)荷比（M/Z）相對(duì)應(yīng)的蛋白質(zhì)。以此分析和研究LBW營養(yǎng)狀況及其蛋白質(zhì)代謝特征。結(jié)果：低出生體重新生兒組與正常出生體重組有顯著的統(tǒng)計(jì)學(xué)意義的蛋白質(zhì)峰有150個(gè)（P0.01）。其中在LBW中低表達(dá)的蛋白質(zhì)峰有98個(gè)，高表達(dá)的蛋白質(zhì)峰有52個(gè)。從分析的結(jié)果看最有研究?jī)r(jià)值（峰值較高，，標(biāo)準(zhǔn)差�。┑牡鞍踪|(zhì)峰有7個(gè)，其質(zhì)荷比（M/Z）分別為2114.94Da、2381.06Da、6835.71Da、9444.83Da、4216.16Da、10421.72Da、5358.07Da。早產(chǎn)低出生體重兒組與足月低出生體重兒組有顯著的統(tǒng)計(jì)學(xué)意義的蛋白質(zhì)峰有74（P0.01）。其中在PLBW中高表達(dá)的蛋白質(zhì)峰有64個(gè)，低表達(dá)的蛋白質(zhì)峰有10個(gè)。從分析的結(jié)果看最有研究?jī)r(jià)值（峰值較高，標(biāo)準(zhǔn)差�。┑牡鞍踪|(zhì)峰有5個(gè)，其質(zhì)荷比（M/Z）分別為2074.52Da、2569.64Da、5349.36Da、5084.58Da、9096.87Da。 結(jié)論:1、通過本次試驗(yàn)得出低出生體重新生兒有蛋白質(zhì)代謝紊亂。2、低出生體重新生兒可能由于體內(nèi)蛋白質(zhì)的表達(dá)缺損或不平衡造成某些蛋白含量較正常出生體重新生兒低或高。早產(chǎn)低出生體重兒可能由于體內(nèi)蛋白質(zhì)的表達(dá)缺損或不平衡造成某些蛋白含量較足月低出生體重兒高或低。3、利用蛋白質(zhì)芯片質(zhì)譜技術(shù)研究低出生體重新生兒蛋白代謝特征可能為低出生體重新生兒的臨床治療提供參考依據(jù)。
[Abstract]:Objective: low birth weight infant, low birth weight (LBW) is defined as less than 2500g of birth weight within 1 hour. Small for gestational age, SGAA (small for gestational age, SGAA) is also called intrauterine growth retardation (IUGRG). In this study, surface enhanced laser desorption ionization enhanced laser desorption/ionization-time of flight-mass spectroscopy (SELDI-TOF-MS) technique was used to study the nutritional status of LBW in order to provide reference for clinical treatment of LBW, to initiate more reasonable treatment scheme and to improve its prognosis as soon as possible. Methods: 91 serum samples were divided into 4 groups, including 59 cases of LBW, 37 cases of premature low birth weight infants, 22 cases of full term low birth weight infants and 32 cases of normal birth weight newborns. Surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS) technique and gold Au-chip were used to collect the fingerprint of serum proteins in each group, and the differential proteins were screened by Biomarker Wizard software. The protein peak-to-mass ratio (P / Z) of these proteins was input into Swiss protein database httpP / us.expasy.org.toolsrtagident.htmln, and the corresponding proteins were obtained. The nutritional status and protein metabolic characteristics of LBW were analyzed and studied. Results: there were 150 protein peaks in low birth weight newborn group and normal birth weight group. There were 98 protein peaks in LBW and 52 protein peaks in high expression. The results show that there are 7 protein peaks with high peak value and low standard deviation, and the ratio of mass to charge is 2114.94 DaC2381.06DaC6835.71Da9444.83Da4216.16DaP10421.72Da5358.07Da. The protein peak of low birth weight preterm infants and term low birth weight infants was 74 (P 0. 01) and that of full term low birth weight infants (P < 0. 01) was significantly higher than that of term low birth weight infants (P < 0. 01). There were 64 high expression protein peaks and 10 low expression protein peaks in PLBW. The results show that there are 5 protein peaks with high peak value and low standard deviation, and the ratio of mass to charge is 2074.52 Da1 2569.64 Da1 5349.36 Da1 5084.58 Da1 9096 87 Da. the results show that there are 5 peaks of protein with high peak value and low standard deviation, and the ratio of mass to charge is 2074.52 Da1, 2569.64 Da1, 5349.36 Da1, 5084.58 Da1. Conclusion: 1. By this experiment, we can conclude that low birth weight newborns have protein metabolism disorder. Low birth weight newborns may have lower or higher protein content than normal birth weight newborns because of protein expression defect or imbalance. The protein content of premature low birth weight infants may be higher or lower than that of full-term low birth weight infants due to protein expression defect or imbalance. Protein chip mass spectrometry was used to study proteins in low birth weight neonates. Metabolic characteristics may provide reference for the clinical treatment of low birth weight newborns.
【學(xué)位授予單位】：瀘州醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R722.1

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