本文選題：Semaphorin5A + 先天性巨結(jié)腸癥　； 參考：《遵義醫(yī)學(xué)院》2012年碩士論文

【摘要】：目的：先天性巨結(jié)腸癥(Hirschsprung disease, HSCR)是一種以腸肌間神經(jīng)叢神經(jīng)節(jié)細(xì)胞缺失為主要特征的疾病,可累計(jì)部分或全部腸管。目前已知腸神經(jīng)元前體細(xì)胞(ENPs)的遷移異常為先天性巨結(jié)腸癥的發(fā)病機(jī)制之一,而Semaphorin5A在ENPs的遷移中起著重要的作用。本研究試圖分析Semaphorin5A蛋白在兒童先天性巨結(jié)腸中的表達(dá)情況,并初步探討Semaphorin5A與先天性巨結(jié)腸癥的關(guān)系。 方法：選取深圳市兒童醫(yī)院手術(shù)治療的先天性巨結(jié)腸癥患兒結(jié)腸標(biāo)本共31例,所有患兒均由手術(shù)切除標(biāo)本送病理活檢確診。將先天性巨結(jié)腸癥患兒的無神經(jīng)節(jié)細(xì)胞的腸組織設(shè)為實(shí)驗(yàn)組,將有神經(jīng)節(jié)細(xì)胞的腸組織設(shè)為對(duì)照組,實(shí)驗(yàn)組的標(biāo)本均經(jīng)過病理檢查證實(shí)無神經(jīng)節(jié),對(duì)照組的標(biāo)本均經(jīng)過病理檢查證實(shí)含有神經(jīng)節(jié)。通過免疫熒光和Western-blot技術(shù)檢測所有患兒的有神經(jīng)節(jié)細(xì)胞的腸組織及無神經(jīng)節(jié)細(xì)胞的腸組織中的Semaphorin5A表達(dá)情況,并將研究數(shù)據(jù)通過t檢驗(yàn)分析,P03.05為差異具有統(tǒng)計(jì)學(xué)意義。 結(jié)果：免疫熒光結(jié)果顯示,Semaphorin5A可表達(dá)于所有標(biāo)本的腸肌層的神經(jīng)節(jié)細(xì)胞、粘膜下層、環(huán)形肌和縱行肌中。Western-blot分析結(jié)果顯示,Semaphorin5A在全部31例患兒的有神經(jīng)節(jié)細(xì)胞的腸組織及無神經(jīng)節(jié)細(xì)胞的腸組織中均有表達(dá),但在無神經(jīng)節(jié)細(xì)胞的腸組織中,Semaphorin5A的表達(dá)水平增高(P0.05)。 結(jié)論：Semaphorin5A的表達(dá)在先天性巨結(jié)腸癥患兒的無神經(jīng)節(jié)細(xì)胞的腸組織中表達(dá)增高,提示Semaphorin5A可能為先天性巨結(jié)腸癥的危險(xiǎn)因素之一。
[Abstract]:Objective: Hirschsprung disease (HSCR) is a disease characterized by the absence of ganglion cells in the intermuscular plexus of the intestine. At present, it is known that the abnormal migration of intestinal neuron precursor cells (ENPs) is one of the pathogenesis of Hirschsprung's disease, and Semaphorin5A plays an important role in the migration of ENPs. This study attempts to analyze the expression of Semaphorin5A protein in children with Hirschsprung's disease and to explore the relationship between Semaphorin5A and Hirschsprung's disease. Methods: a total of 31 children with Hirschsprung's disease underwent surgical treatment in Shenzhen Children's Hospital. The intestinal tissues of children with Hirschsprung's disease were divided into experimental group and control group. The specimens of the experimental group were confirmed by pathological examination. The specimens of the control group were confirmed to contain ganglion by pathological examination. Immunofluorescence and Western-blot techniques were used to detect the expression of Semaphorin5A in ganglion cells and non-ganglion cells in all children, and the difference was statistically significant by t-test analysis (P03.05). Results: the results of immunofluorescence showed that Semaphorin5A could be expressed in ganglion cells and submucosal cells in the myenteric layer of all specimens. Western-blot analysis of ring and longitudinal muscles showed that Semaphorin5A was expressed in all 31 children with ganglion cells and without ganglion cells, but the expression level of Semaphorin 5A was increased in the intestinal tissues without ganglion cells. Conclusion the expression of Semaphorin5A in the intestine of children with Hirschsprung's disease is increased, suggesting that Semaphorin5A may be one of the risk factors of Hirschsprung's disease.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R726.5

【共引文獻(xiàn)】

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1 Adam S Wallace;Richard B Anderson;;Genetic interactions and modifi er genes in Hirschsprung's disease[J];World Journal of Gastroenterology;2011年45期

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1 張華;MITF、PAX3和SOX10基因突變致Waardenburg綜合征發(fā)病的分子機(jī)制研究[D];中南大學(xué);2012年

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1 臧冬東;RET基因多態(tài)性與先天性巨結(jié)腸患病風(fēng)險(xiǎn)研究[D];遵義醫(yī)學(xué)院;2012年

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本文編號(hào)：1990751