本文選題：持續(xù)性肺動(dòng)脈高壓 + 鈣敏感受體��； 參考：《中國(guó)當(dāng)代兒科雜志》2017年02期

【摘要】：目的探討鈣敏感受體(CaSR)激動(dòng)劑及抑制劑在新生小鼠持續(xù)性肺動(dòng)脈高壓(PPH)模型中對(duì)CaSR的表達(dá)影響,明確其在新生小鼠PPH模型中的作用。方法將49只新生小鼠隨機(jī)分為對(duì)照組(n=10)、PPH組(n=11)、激動(dòng)劑組(n=13)和抑制劑組(n=15);將PPH組、激動(dòng)劑組和抑制劑組小鼠暴露在12%的氧濃度中,對(duì)照組小鼠暴露在空氣中。激動(dòng)劑組和抑制劑組每日分別給予GdCl3(16 mg/kg)、NPS2390(1 mg/kg)腹腔注射1次,低氧組和對(duì)照組每日以生理鹽水替代,共持續(xù)14 d。采用蘇木精-伊紅染色和免疫組化檢測(cè)肺血管的變化;采用激光共聚焦技術(shù)觀察CaSR在新生小鼠肺組織中的表達(dá)位置及含量;qRT-PCR技術(shù)檢測(cè)新生小鼠肺組織中CaSR mRNA表達(dá);Western blot法檢測(cè)新生小鼠肺組織中CaSR蛋白的表達(dá)。結(jié)果與對(duì)照組相比,PPH組肺小動(dòng)脈血管壁厚度(WT%)及右心室與左心室壁厚度比(RV/LV)均較對(duì)照組明顯增大(P0.05),模型驗(yàn)證成功。與對(duì)照組相比,PPH組CaSR mRNA和蛋白表達(dá)水平明顯增加(P0.05),激動(dòng)劑組表達(dá)水平增加更加明顯(P0.05),而抑制劑組表達(dá)減少(均P0.05)。結(jié)論 CaSR參與了低氧誘導(dǎo)的新生小鼠PPH,可能發(fā)揮重要作用。
[Abstract]:Objective to investigate the effect of calcium sensitive receptor (CASR) agonists and inhibitors on the expression of CaSR in neonatal mice with persistent pulmonary hypertension (PPH). Methods Forty-nine newborn mice were randomly divided into control group (n = 10), agonist group (n = 13) and inhibitor group (n = 15), PPH group, agonist group and inhibitor group were exposed to 12% oxygen concentration, while control group mice were exposed to air. The agonist group and the inhibitor group received intraperitoneal injection of GdCl3(16 mg / kg / kg NPS2390 mg / kg, respectively. The hypoxic group and the control group were replaced by normal saline daily for 14 days. The changes of pulmonary blood vessels were detected by hematoxylin-eosin staining and immunohistochemistry. The expression and content of CaSR in the lung tissue of newborn mice were observed by laser confocal technique. The expression of CaSR mRNA in lung tissue of newborn mice was detected by qRT-PCR. The expression of CaSR protein in lung tissue of newborn mice was detected by Western blot method. Results compared with the control group, the pulmonary arteriole wall thickness (WTT) and the ratio of right ventricular to left ventricular wall thickness (RV / LVV) in PPH group were significantly larger than those in control group (P 0.05). The model was successfully verified. Compared with the control group, the expression of CaSR mRNA and protein in the PPH group was significantly increased, the expression level in the agonist group was significantly higher than that in the control group, but the expression level in the inhibitor group was decreased (P 0.05). Conclusion CaSR may play an important role in hypoxic-induced neonatal mice.
【作者單位】： 石河子大學(xué)醫(yī)學(xué)院第一附屬醫(yī)院兒科;石河子大學(xué)醫(yī)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(81560257)
【分類號(hào)】：R722.19

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本文編號(hào)：1982328