本文選題：急性淋巴細胞白血病 + 兒童��； 參考：《鄭州大學》2014年碩士論文

【摘要】：研究背景 急性淋巴細胞白血�。╝cute lymphoid leukemia，ALL）是兒童時期最常見的血液腫瘤，占兒童白血病的70%左右，目前發(fā)病機制尚不明確。而有研究發(fā)現(xiàn)控制生物節(jié)律的內(nèi)在生物鐘基因表達異常與腫瘤的發(fā)生相關(guān)，在急慢性髓系白血病及非霍奇金淋巴瘤中都有檢測到鐘基因表達異�！，F(xiàn)有關(guān)鐘基因表達與急性淋巴細胞白血病發(fā)病關(guān)系的研究較少見。 研究目的 通過對急性淋巴細胞白血病患兒生物鐘基因Bmal1、Per2基因表達的檢測，探討生物鐘基因在兒童急性淋巴細胞白血病中的表達，初步揭示鐘基因在急性淋巴細胞白血病發(fā)病中的作用。 研究對象和方法 1.選擇鄭州大學第一附屬醫(yī)院兒童血液科ALL初診未治療患兒30例為實驗組1（ALL初治組），ALL化療完全緩解患兒30例為實驗組2（ALL-CR組），體檢中心體檢兒童30例為健康對照組。實驗組入選標準參照2006年“兒童急性淋巴細胞白血病診療建議（第3次修訂草案）”診斷分型標準，實驗組和對照組均排除肥胖、心血管疾病、腫瘤及睡眠周期紊亂等影響鐘基因表達的疾病。 2.于晨06:00用無菌注射器分別抽取實驗組和對照組空腹靜脈血各2ml，并迅速打入EDTA抗凝管中，輕輕搖勻。 3.應用密度梯度離心法分離外周血單個核細胞，，吸取白膜層。 4.按照Trizol試劑說明書提取外周血單個核細胞總RNA，檢測RNA的純度及完整性；應用反轉(zhuǎn)錄試劑盒合成cDNA；采用Real-time PCR測定Bmal1mRNA、Per2mRNA的表達。 結(jié)果 1. ALL初治組患兒Bmal1mRNA、Per2mRNA表達與健康對照組比較，差異有統(tǒng)計學意義（P0.05），ALL初治組患兒Per2mRNA表達量水平均較對照組減低，Bmal1mRNA表達水平均較健康對照組增高。 2. ALL-CR組患兒Bmal1mRNA、Per2mRNA表達與健康對照組比較差異有統(tǒng)計學意義（P0.05），ALL-CR組Bmal1mRNA、Per2mRNA表達均較健康對照組減低。 3. ALL-CR組患兒Bmal1mRNA、Per2mRNA表達與ALL初治組比較，差異有統(tǒng)計學意義（P0.05），ALL-CR組Bmal1mRNA、Per2mRNA表達均較ALL初治組減低。 結(jié)論 1.在兒童ALL外周血單個核細胞中存在生物鐘基因Bmal1mRNA、Per2mRNA異常表達,表明其可能在兒童ALL的發(fā)生、發(fā)展中發(fā)揮一定作用； 2.化療藥物應用可影響鐘基因的表達。
[Abstract]:Research background Acute lymphoid leukemia (ALL) is the most common blood tumor in childhood, accounting for about 70% of childhood leukemia. Some studies have found that the abnormal expression of the internal clock gene in biological rhythms is related to the occurrence of tumor. The abnormal expression of clock gene has been detected in both acute and chronic myeloid leukemia and non-Hodgkin 's lymphoma. There are few studies on the relationship between bell gene expression and acute lymphoblastic leukemia. Research purpose The expression of biological clock gene (Bmal1PER2) in children with acute lymphoblastic leukemia (AML) was studied, and the role of clock gene in the pathogenesis of acute lymphoblastic leukemia (ALL) was preliminarily revealed. Research objects and methods 1. Thirty untreated children with ALL in Department of Children's Hematology, first affiliated Hospital of Zhengzhou University were selected as experimental group, 30 children with complete remission of all chemotherapy in 1(ALL group, 30 children with complete remission of all chemotherapy in experimental group, and 30 children in physical examination center as healthy control group. According to the criteria for diagnosis and treatment of childhood acute lymphoblastic leukemia (the third revised draft) in 2006, the experimental group and the control group were excluded from obesity and cardiovascular disease. Cancer and sleep cycle disorders affect the expression of clock gene diseases. 2. At 06:00, the fasting venous blood of the experimental group and the control group were extracted with sterile syringe 2 ml each, and the venous blood was quickly injected into the EDTA anticoagulant tube and gently shaken. 3. The peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation. 4. Total RNAs of peripheral blood mononuclear cells were extracted according to Trizol reagent instructions to detect the purity and integrity of RNA. Reverse transcription kit was used to synthesize cDNA. Real-time PCR was used to detect the expression of Bmal1mRNA-Per2 mRNA. Result 1. The expression of Bmal1mRNA-Per2 mRNA in the ALL group was significantly higher than that in the control group (P 0.05). The expression of Bmal1 mRNA in the primary treatment group was significantly higher than that in the control group. 2. The expression of Bmal1mRNA-Per2 mRNA in ALL-CR group was significantly lower than that in healthy control group. The expression of Bmal1mRNA-Per2 mRNA in ALL-CR group was significantly lower than that in healthy control group. 3. The expression of Bmal1mRNA-Per2mRNA in ALL-CR group was significantly lower than that in ALL group. The expression of Bmal1mRNA-Per2mRNA in ALL-CR group was lower than that in ALL group. Conclusion 1. The abnormal expression of Bmal1mRNA-Per2 mRNA in peripheral blood mononuclear cells of children with ALL suggests that Bmal1mRNA-Per2 mRNA may play a role in the occurrence and development of ALL in children. 2. The application of chemotherapeutic drugs can affect the expression of bell gene.
【學位授予單位】：鄭州大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R733.71

【參考文獻】

相關(guān)期刊論文 前3條

1 楊福全;王澤平;王鑫;叢玉珠;馬銳;張智深;孟令勤;趙海鷹;劉金鋼;;生物鐘基因Clock、Bmal1蛋白在膽管癌組織中的表達及臨床意義[J];中國現(xiàn)代普通外科進展;2014年02期

2 蔣莎義,陳力軍,高飛,李桂梅,劉廷亮,尹洪臣;癌基因MDM2過度表達與兒童急性淋巴細胞白血病的發(fā)病和預后的關(guān)系[J];中華兒科雜志;2000年07期

3 鄧香群;張鵬飛;賀印旎;;生物鐘基因hClock hBmal 1在腫瘤中的表達研究[J];中國腫瘤臨床;2011年10期

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