本文選題：氯胺酮 + 海馬��； 參考：《安徽醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的：探討氯胺酮對新生大鼠學(xué)習(xí)記憶能力和海馬乙酰膽堿酯酶(AchE)、乙酰膽堿轉(zhuǎn)移酶(ChAT)的影響。 方法：80只7d齡SD大鼠，，體重12～18g，雌雄不拘，隨機(jī)分為C、K1、K2、K3組，每組20只。C組單次腹腔注射（i.p.）生理鹽水2ml；K1組單次注射氯胺酮80mg/kg i.p.；K2組連續(xù)2d給予氯胺酮，80mg/kg.d i.p.，K3組連續(xù)3d給予氯胺酮，80mg/kg.d i.p.。給藥過程結(jié)束后1d各組處死大鼠10只（首批處死）并立即取左、右腦海馬組織以比色法分別觀察乙酰膽堿轉(zhuǎn)移酶、乙酰膽堿酯酶表達(dá)情況；其余各組大鼠飼養(yǎng)至21d齡采用Morris水迷宮系統(tǒng)觀察學(xué)習(xí)記憶能力改變情況，水迷宮行為測試結(jié)束后處死大鼠（27d齡處死），立即取左、右腦海馬組織比色法分別觀察ChAT、AchE表達(dá)情況。大鼠的海馬組織ChAT、AchE活力和學(xué)習(xí)記憶能力變化結(jié)果采用統(tǒng)計軟件SPSS13.0分析比較。 結(jié)果：⑴Morris水迷宮：C組與K1組之間比較、K2組與K3組之間比較差異均無顯著性(P0.05)；K2、K3組較C、K1組潛伏期延長且穿越平臺次數(shù)減少，差異有顯著性(P0.05)。⑵酶測定：首批處死后，C、K1、K2、K3各組間差異有顯著性(P0.05)。27d齡處死，C組與K1組之間比較、K2組與K3組之間比較差異均無顯著性(P0.05)；K2、K3組較C、K1組差異均有顯著性(P0.05)。 結(jié)論：氯胺酮80mg/kg可引起7日齡SD大鼠海馬膽堿能系統(tǒng)ChAT、AchE活力改變。單次給藥可引起大鼠24h內(nèi)海馬膽堿能系統(tǒng)ChAT、AchE活力變化；重復(fù)給藥可引起24h內(nèi)及21天后海馬膽堿能系統(tǒng)ChAT、AchE活力變化；隨著給藥次數(shù)增加所引起的24h內(nèi)海馬膽堿能系統(tǒng)ChAT、AchE活力變化也不相同。單次給藥未見引起大鼠21天后空間學(xué)習(xí)記憶能力改變；重復(fù)給藥能引起21天后空間學(xué)習(xí)記憶能力改變；而重復(fù)給藥后再次給藥并未見明顯ChAT、AchE與空間學(xué)習(xí)記憶能力改變。SD大鼠海馬膽堿能系統(tǒng)ChAT、AchE參與了空間學(xué)習(xí)記憶功能，氯胺酮引起空間學(xué)習(xí)記憶能力的改變可能與其影響海馬膽堿能神經(jīng)乙酰膽堿酯酶、乙酰膽堿轉(zhuǎn)移酶有關(guān)。
[Abstract]:Aim: to investigate the effects of ketamine on learning and memory ability and hippocampal acetylcholinesterase (ache) in neonatal rats. Methods Twenty seven day old SD rats, weighing 12g or 18g, were randomly divided into two groups: group C (n = 20) and group C (n = 20). Normal saline 2ml / L K1 group was given Ketamine 80 mg / kg / kg i.p. K3 for 3 consecutive days by single injection of ketamine 80mg/kg i.p. K2 for 2 consecutive days, and ketamine 80 mg / kg 路d i.p. Ten rats in each group were killed 1 day after the administration (the first group was executed) and the expression of acetylcholine transferase and acetylcholinesterase were observed by colorimetric method in the hippocampus of left and right brain. Morris water maze system was used to observe the changes of learning and memory ability in the other groups. After the water maze behavior test, the rats were killed at the age of 27 days, and the expression of Chattoache was observed by colorimetric method in the hippocampus of left and right brain. The changes of ache activity and learning and memory ability in hippocampus of rats were analyzed and compared by SPSS13.0 software. Results there was no significant difference between K2 group and K3 group compared with K1 group. The latency of K2 group was longer and the number of crossing platform was lower than that of Con K1 group, and there was no significant difference between K3 group and K1 group, and there was no significant difference between K2 group and K1 group, compared with K1 group, there was no significant difference between K2 group and K3 group. There was a significant difference in P0.05.2 enzyme: after the first group was executed, there was significant difference among the three groups. There was no significant difference between K2 group and K3 group compared with K1 group. There was no significant difference in P0.05K2K3 group and CnK1 group in comparison with that in C0. 05K1 group. There was no significant difference between K2 group and K3 group. Conclusion: ketamine 80mg/kg can induce the changes of cholinergic system activity of cholinergic system in 7 day old SD rats. The changes of Chatache activity in hippocampal cholinergic system were induced by single administration of the drug within 24 hours, and the activity of Chatache in hippocampal cholinergic system by repeated administration within 24 hours and 21 days after administration. The changes of ChATache activity in hippocampal cholinergic system were different with the increase of administration times in 24 hours. No change of spatial learning and memory ability was found in rats after 21 days of single administration, but repeated administration of drugs caused changes in spatial learning and memory ability after 21 days. However, after repeated administration, there was no obvious difference between ChATAache and spatial learning and memory ability. The cholinergic system ChATAache in hippocampus of SD rats was involved in spatial learning and memory function. The change of spatial learning and memory induced by ketamine may be related to the effect of ketamine on cholinergic acetylcholinesterase and acetylcholine transferase in hippocampal cholinergic nerve.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R965;R722.1

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