本文選題：呼吸道感染 + 病毒入侵��； 參考：《濟(jì)南大學(xué)》2017年碩士論文

【摘要】：病毒性呼吸道感染是呼吸道感染的主要組成部分。干擾素誘導(dǎo)的跨膜家族參與細(xì)胞的多種活動。人IFITMs家族蛋白有5類分別為IFITM1、IFITM2、IFITM3、IFITM5和IFITM10,均位于11號染色體。IFITM家族可以分為三類,一類是與免疫相關(guān)的IFITM1、IFITM2和IFITM3,參與抵抗病毒入侵的細(xì)胞免疫階段。第二類是骨限制性表達(dá)的蛋白IFITM5,與骨中鈣鹽的沉積有關(guān)。第三類IFITM10可能與水環(huán)境適應(yīng)性有關(guān)。鼠IFITMs家族中還有IFITM6和IFITM7,除IFITM7位于16號染色體外,其余均位于7號染色體。IFITM家族調(diào)節(jié)細(xì)胞的增殖和促進(jìn)細(xì)胞的同性粘附,它們在多種腫瘤中過表達(dá),與腫瘤細(xì)胞的遷移、侵襲和增殖密切相關(guān)。IFITM1、IFITM2和IFITM3參與抵抗病毒的入侵。IFITMs定位于細(xì)胞膜表面。IFITM1抵抗絲狀病毒最有效,IFITM3抵抗甲型流感最有效。IFITM3定位于細(xì)胞晚期內(nèi)含體途徑,依賴于IFITM3蛋白的膜內(nèi)區(qū)域1介導(dǎo)的活動,并且通過N-末端和IFITM蛋白中的保守的細(xì)胞內(nèi)環(huán)路共同協(xié)作,最大化的限制病毒的入侵。IFITMs家族在生命活動中具有重要作用,在生物體內(nèi)發(fā)生的功能作用及其機(jī)制還需要進(jìn)一步研究。目的:分析呼吸道感染樣本中IFITMs的表達(dá)差異,探尋IFITMs蛋白與呼吸道病毒感染的關(guān)系;參考大鼠組織IFITMs基因表達(dá),分析IFITMs表達(dá)譜系。方法:(1)對下呼吸道感染樣本進(jìn)行臨床信息分類匯總以及呼吸道病毒感染匯總。(2)設(shè)計(jì)并篩選人類IFITMs引物,通過RT-qPCR對下呼吸道感染樣本中IFITMsmRNA進(jìn)行擴(kuò)增,分析IFITMs的相對表達(dá)。(3)提取大鼠組織RNA,設(shè)計(jì)并篩選大鼠IFITMs引物,利用RT-qPCR分析大鼠32種組織IFITMs表達(dá)。結(jié)果:本研究對320例兒童下呼吸道感染樣本進(jìn)行分析。發(fā)現(xiàn)IFITM3在支氣管肺炎與重癥肺炎、輕癥肺炎與重癥肺炎分類中的表達(dá)差異有統(tǒng)計(jì)學(xué)意義。IFITM3對流感病毒的限制能力和IFITM1對冠狀病毒的限制能力區(qū)別于其它病毒。從呼吸道感染患者的肺泡灌洗液中提取的核酸,發(fā)現(xiàn)了IFITM5在mRNA水平上有表達(dá)。大鼠32種組織IFITMs mRNA表達(dá)結(jié)果表明IFITM1、IFITM2和IFITM3在各組中的表達(dá)比較普遍。在IFITM5的表達(dá)圖譜中發(fā)現(xiàn)除了在骨骼中有分布,如骨髓、皮膚、胰和平滑肌等組織中均有表達(dá)。IFITM6在眼睛中的相對表達(dá)量最高,IFITM2與IFITM7的有類似的表達(dá)趨勢。IFITM3、IFITM5和IFITM10在平滑肌中表達(dá)量均明顯高表達(dá)與骨骼肌。結(jié)論:IFITM3蛋白在呼吸道感染加重過程中的相對表達(dá)發(fā)生變化。不同的IFITMs成員對同一病毒的限制能力不同。IFITMs限制病毒入侵的機(jī)制需進(jìn)一步研究。在大鼠組織IFITMs表達(dá)譜系中,發(fā)現(xiàn)IFITM5在骨骼中的表達(dá)存在物種差異。由于同源性的關(guān)系,IFITM2和IFITM7,IFITM5和IFITM10在組織的表達(dá)圖譜中有很多相似的地方,但是其在組織中的功能尚需進(jìn)一步研究。
[Abstract]:Viral respiratory tract infection is the main component of respiratory tract infection. Interferon-induced transmembrane families are involved in a variety of cellular activities. There are five types of human IFITMs family proteins, IFITM1, IFITM2, IFITM3, IFITM5 and IFITM10, which are located on chromosome 11. IFITM1, IFITM2 and IFITM3, which are immune-related, are involved in the cellular immunity against virus invasion. The second is bone restricted protein IFITM 5, which is related to the deposition of calcium salt in bone. The third kind of IFITM10 may be related to the adaptability of water environment. IFITM6 and IFITM7 are also found in the IFITMs family of mice. Except for IFITM7 on chromosome 16, the rest are located on chromosome 7. IFITM family regulates the proliferation of cells and promotes the same sex adhesion of cells. They are overexpressed in many kinds of tumors and are related to the migration of tumor cells. Invasion and proliferation are closely related to. IFITM1IFITM2 and IFITM3 are involved in resisting virus invasiveness. IFITMs are located on the surface of cell membrane. IFITM1 is the most effective anti-filamentous virus. IFITM3 is the most effective anti-influenza A virus. IFITM3 is located in the late stage of cell inclusion pathway. Dependent on the intramembrane domain 1 of IFITM3 protein, and through the cooperation of N- terminal and conserved intracellular loop in IFITM protein, the restriction of virus invasion. IFITMs family plays an important role in life activities. The function and mechanism of the function in organism need to be further studied. Aim: to analyze the difference of IFITMs expression in respiratory tract infection samples, to explore the relationship between IFITMs protein and respiratory virus infection, and to analyze the IFITMs expression lineage by referring to the expression of IFITMs gene in rat tissues. Methods the human IFITMs primers were designed and screened for the clinical information classification of lower respiratory tract infection samples and respiratory virus infection collection. The IFITMsmRNA was amplified by RT-qPCR in the lower respiratory tract infection samples. The relative expression of IFITMs in rat tissues was analyzed. The rat IFITMs primers were designed and screened. RT-qPCR was used to analyze the expression of IFITMs in 32 tissues of rats. Results: 320 children with lower respiratory tract infection were analyzed. It was found that there were significant differences in the expression of IFITM3 between bronchopneumonia and severe pneumonia, mild pneumonia and severe pneumonia. The limiting ability of IFITM3 to influenza virus and IFITM1 to coronavirus were different from those of other viruses. Nucleic acid extracted from alveolar lavage fluid of patients with respiratory tract infection showed that IFITM5 was expressed at mRNA level. The expression of IFITMs mRNA in 32 tissues of rats showed that IFITM1, IFITM2 and IFITM3 were more common in each group. In the IFITM5 expression profile, it was found that except in the bone, such as bone marrow, skin, The relative expression of IFITM6 in the eyes was the highest in pancreatic and smooth muscle tissues. IFITM2 and IFITM7 had similar expression trend. IFITM3, IFITM5 and IFITM10 were highly expressed in smooth muscle and skeletal muscle. Conclusion the relative expression of the fraction IFITM3 protein changes during the exacerbation of respiratory tract infection. Different IFITMs members have different limiting ability to the same virus. In the IFITMs expression lineage of rat tissues, species differences were found in the expression of IFITM5 in bone. There are many similarities between IFITM2 and IFITM7 IFITM5 and IFITM10 in the tissue expression profiles due to homology, but their functions in tissues need further study.
【學(xué)位授予單位】：濟(jì)南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R725.6

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