本文選題：轉(zhuǎn)化生長(zhǎng)因子-β + 白細(xì)胞介素-10　； 參考：《山東大學(xué)》2012年碩士論文

【摘要】：目的： 毛細(xì)支氣管炎是嬰幼兒最常見(jiàn)的肺間質(zhì)性炎癥,在毛細(xì)支氣管炎病原體中,呼吸道合胞病毒最常見(jiàn),占70%以上。炎性因子介導(dǎo)的炎癥反應(yīng)在毛細(xì)支氣管炎的發(fā)病機(jī)制中有重要作用。本實(shí)驗(yàn)檢測(cè)呼吸道合胞病毒毛細(xì)支氣管炎患兒外周血中轉(zhuǎn)化生長(zhǎng)因子-β(TGF-β)和白細(xì)胞介素-10(IL-10)濃度的變化,并探討其臨床意義。 研究對(duì)象和方法： 選擇自2011年10月至2012年4月在聊城市人民醫(yī)院住院的呼吸道合胞病毒毛細(xì)支氣管炎患兒80例(咽拭子呼吸道合胞病毒-PCR陽(yáng)性者)作為研究對(duì)象,根據(jù)是否合并心力衰竭,分為毛細(xì)支氣管炎組(簡(jiǎn)稱(chēng)普通組)和毛細(xì)支氣管炎組合并心衰組(簡(jiǎn)稱(chēng)心衰組),40例健康兒童作為對(duì)照組。應(yīng)用ELISA雙抗體夾心法檢測(cè)各組兒童外周血TGF-β和IL-10的含量。 結(jié)果： 1.治療前,普通組外周血TGF-β含量(21.77±4.47pg/ml)和心衰組外周血TGF-β含量(14.86±3.72pg/ml),均明顯低于對(duì)照組(53.78±4.10pg/ml)(p均0.05)。 2.治療前,心衰組外周血TGF-β水平(14.86±3.72pg/ml)明顯低于普通組(21.77±4.47pg/ml)(p0.05)。 3.治療前,普通組外周血IL-10含量(17.55±2.47pg/ml)、心衰組外周血IL-10含量(10.80±2.72pg/ml),均明顯低于對(duì)照組(47.52±4.10pg/ml)(p均0.05)。 4.治療前,心衰組外周血IL-10水平(10.80±2.72pg/m1)明顯低于普通組(17.55±2.47pg/m1)(p0.05)。 5.治療后,普通組外周血TGF-β含量(46.21±5.77pg/m1)較治療前(21.77±4.47pg/m1)明顯增高(p均0.05)。心衰組外周血TGF-β含量(35.68±4.03pg/m1)亦較治療前(14.86±3.72pg/m1)明顯增高(p均0.05)。 6.治療后,心衰組外周血TGF-β水平(35.68±4.03pg/m1)仍低于普通組(46.21±5.77pg/m1)(p均0.05)。 7.治療后,普通組外周血IL-10含量(30.50±2.77pg/m1)較治療前(17.55±2.47pg/m1)明顯增高(p均0.05),心衰組外周血IL-10含量(21.93±3.03pg/m1)亦較治療前(10.80±2.72pg/m1)明顯增高(p均0.05)。 8.治療后,心衰組外周血IL-10水平(21.93±3.03pg/m1)仍低于普通組(30.50±2.77pg/m1)(p0.05)。 結(jié)論： 呼吸道合胞病毒毛細(xì)支氣管炎患兒急性期TGF-β和IL-10含量均明顯降低,合并心力衰竭患兒尤為顯著�；謴�(fù)期TGF-β和IL-10含量較急性期均明顯升高,但濃度仍低于正常兒童。說(shuō)明TGF-β、IL-10在毛細(xì)支氣管炎的發(fā)病過(guò)程中起重要作用。外周血TGF-β、IL-10水平可作為毛細(xì)支氣管炎病情輕重的指標(biāo)。
[Abstract]:Objective: Bronchiolitis is the most common pulmonary interstitial inflammation in infants. Respiratory syncytial virus (RSV) is the most common pathogen of bronchiolitis, accounting for more than 70%. Inflammatory cytokines play an important role in the pathogenesis of bronchiolitis. The changes of TGF- 尾 and IL-10 in peripheral blood of children with respiratory syncytial virus capillary bronchitis were detected and its clinical significance was discussed. Subjects and methods of study: From October 2011 to April 2012, 80 children with respiratory syncytial virus capillary bronchitis (throat swab respiratory syncytial virus -PCR positive) hospitalized in Liaocheng people's Hospital were selected as study subjects, according to whether they were complicated with heart failure. They were divided into bronchiolitis group (normal group) and bronchiolitis combined with heart failure group (40 healthy children) as control group. The levels of TGF- 尾 and IL-10 in peripheral blood of children in each group were detected by ELISA double antibody sandwich method. Results: 1. Before treatment, the level of TGF- 尾 in peripheral blood of normal group and heart failure group was 21.77 鹵4.47pg / ml and 14.86 鹵3.72pg / ml respectively, which was significantly lower than that of control group (53.78 鹵4.10pg/ml)(p). 2. Before treatment, the level of TGF- 尾 in peripheral blood of CHF group was 14.86 鹵3.72 PG / ml significantly lower than that of normal group (21.77 鹵4.47 PG / ml). 3. Before treatment, the IL-10 content in peripheral blood of normal group was 17.55 鹵2.47 PG / ml, and that of peripheral blood IL-10 in heart failure group was 10.80 鹵2.72 PG / ml, which was significantly lower than that in control group (47.52 鹵4.10pg/ml)(p). 4. Before treatment, the level of IL-10 in peripheral blood of CHF group was significantly lower than that of normal group (10.80 鹵2.72pg / ml). 5. After treatment, the levels of TGF- 尾 in peripheral blood of the normal group were significantly higher than those of the control group (46.21 鹵5.77pg / ml) and 21.77 鹵4.47pg / ml, respectively. The level of TGF- 尾 in peripheral blood of heart failure group was 35.68 鹵4.03pg / m ~ (-1) significantly higher than that before treatment (14.86 鹵3.72 PG / m ~ (-1)). 6. After treatment, the level of TGF- 尾 in peripheral blood of CHF group was 35.68 鹵4.03pg / ml, which was still lower than that of normal group (46.21 鹵5.77pg/m1)(p, 0.05g / ml). 7. After treatment, the level of IL-10 in peripheral blood of normal group (30.50 鹵2.77pg / ml) was significantly higher than that of pre-treatment (17.55 鹵2.47pg / ml). The IL-10 content in peripheral blood of heart failure group was 21.93 鹵3.03pgP 路m1), which was significantly higher than that before treatment (10.80 鹵2.72pg / ml). 8. After treatment, the level of IL-10 in peripheral blood of CHF group (21.93 鹵3.03pg / ml) was still lower than that of normal group (30.50 鹵2.77pg / ml). Conclusion: The levels of TGF- 尾 and IL-10 in children with respiratory syncytial virus capillary bronchitis decreased significantly in acute phase, especially in children with heart failure. The levels of TGF- 尾 and IL-10 in convalescent stage were significantly higher than those in acute stage, but the concentration of TGF- 尾 and IL-10 was still lower than that of normal children. The results suggest that TGF- 尾 -IL-10 plays an important role in the pathogenesis of bronchiolitis. The level of TGF- 尾 -IL-10 in peripheral blood can be used as an indicator of the severity of bronchiolitis.
【學(xué)位授予單位】：山東大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R725.6

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