本文選題：皮質(zhì)酮 + 腦發(fā)育��； 參考：《中國當代兒科雜志》2017年09期

【摘要】：目的研究皮質(zhì)酮對發(fā)育中胎鼠腦皮層神經(jīng)元遷移蛋白Lissencephaly 1(LIS1)表達的影響。方法體外原代培養(yǎng)Wistar胎鼠腦皮層神經(jīng)元并分為對照組、低濃度干預組和高濃度干預組,各組給予含有不同濃度皮質(zhì)酮(對照組:0μmol/L,低濃度干預組:0.1μmol/L,高濃度干預組:1.0μmol/L)的培養(yǎng)基干預,分別于干預后1、4、7 d收集細胞,采用Werstern blot法及免疫細胞化學染色法觀察神經(jīng)元LIS1蛋白表達的變化。結果 Western blot結果顯示干預后7 d,低濃度干預組及高濃度干預組胎鼠腦皮層神經(jīng)元胞漿及胞核內(nèi)LIS1表達均明顯低于對照組(P0.05),且高濃度干預組胞漿內(nèi)LIS1表達明顯低于低濃度干預組(P0.05)。免疫細胞化學染色法結果顯示皮質(zhì)酮干預后1、4、7 d,高濃度干預組LIS1平均光密度值明顯低于對照組及低濃度干預組(P0.05),且干預后7 d,低濃度干預組LIS1平均光密度值明顯低于對照組(P0.05)。結論皮質(zhì)酮可下調(diào)體外培養(yǎng)發(fā)育中胎鼠腦皮層神經(jīng)元遷移蛋白LIS1的表達水平,且與皮質(zhì)酮濃度高低、作用時間長短相關。
[Abstract]:Objective to study the effect of corticosterone on the expression of neuronal migration protein Lissencephaly 1 (LIS1) in cortical neurons of developing fetus. Methods Wistar fetal cortical neurons were cultured in vitro and divided into control group, low concentration intervention group and high concentration intervention group. Each group was treated with different concentrations of corticosterone (control group: 0 渭 mol / L, low concentration group: 0.1 渭 mol / L, high concentration group: 1 渭 mol / L). The expression of LIS1 protein in neurons was observed by Werstern blot assay and immunocytochemical staining. Results the results of Western blot showed that the expression of LIS1 in the cytoplasm and nucleus of cortical neurons in the low concentration group and the high concentration group was significantly lower than that in the control group on the 7th day after the intervention, and the expression of LIS1 in the cytoplasm of the high concentration intervention group was significantly lower than that of the low concentration intervention group. The results of immunocytochemical staining showed that the average optical density of LIS1 in the high concentration intervention group was significantly lower than that in the control group and the low concentration intervention group on the 7th day after the intervention of corticosterone, and the average optical density of LIS1 in the low concentration intervention group was significantly lower than that in the control group at 7 days. Conclusion corticosterone can down-regulate the expression of migration protein LIS1 in cortical neurons of developing fetus in vitro, which is related to the concentration of corticosterone and the duration of action.
【作者單位】： 中南大學湘雅二醫(yī)院兒童醫(yī)學中心;
【基金】：國家自然科學基金(81671506)
【分類號】：R722.1

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