本文選題：皮質(zhì)酮 + 腦發(fā)育　； 參考：《中國(guó)當(dāng)代兒科雜志》2017年09期

【摘要】：目的研究皮質(zhì)酮對(duì)發(fā)育中胎鼠腦皮層神經(jīng)元遷移蛋白Lissencephaly 1(LIS1)表達(dá)的影響。方法體外原代培養(yǎng)Wistar胎鼠腦皮層神經(jīng)元并分為對(duì)照組、低濃度干預(yù)組和高濃度干預(yù)組,各組給予含有不同濃度皮質(zhì)酮(對(duì)照組:0μmol/L,低濃度干預(yù)組:0.1μmol/L,高濃度干預(yù)組:1.0μmol/L)的培養(yǎng)基干預(yù),分別于干預(yù)后1、4、7 d收集細(xì)胞,采用Werstern blot法及免疫細(xì)胞化學(xué)染色法觀察神經(jīng)元LIS1蛋白表達(dá)的變化。結(jié)果 Western blot結(jié)果顯示干預(yù)后7 d,低濃度干預(yù)組及高濃度干預(yù)組胎鼠腦皮層神經(jīng)元胞漿及胞核內(nèi)LIS1表達(dá)均明顯低于對(duì)照組(P0.05),且高濃度干預(yù)組胞漿內(nèi)LIS1表達(dá)明顯低于低濃度干預(yù)組(P0.05)。免疫細(xì)胞化學(xué)染色法結(jié)果顯示皮質(zhì)酮干預(yù)后1、4、7 d,高濃度干預(yù)組LIS1平均光密度值明顯低于對(duì)照組及低濃度干預(yù)組(P0.05),且干預(yù)后7 d,低濃度干預(yù)組LIS1平均光密度值明顯低于對(duì)照組(P0.05)。結(jié)論皮質(zhì)酮可下調(diào)體外培養(yǎng)發(fā)育中胎鼠腦皮層神經(jīng)元遷移蛋白LIS1的表達(dá)水平,且與皮質(zhì)酮濃度高低、作用時(shí)間長(zhǎng)短相關(guān)。
[Abstract]:Objective to study the effect of corticosterone on the expression of neuronal migration protein Lissencephaly 1 (LIS1) in cortical neurons of developing fetus. Methods Wistar fetal cortical neurons were cultured in vitro and divided into control group, low concentration intervention group and high concentration intervention group. Each group was treated with different concentrations of corticosterone (control group: 0 渭 mol / L, low concentration group: 0.1 渭 mol / L, high concentration group: 1 渭 mol / L). The expression of LIS1 protein in neurons was observed by Werstern blot assay and immunocytochemical staining. Results the results of Western blot showed that the expression of LIS1 in the cytoplasm and nucleus of cortical neurons in the low concentration group and the high concentration group was significantly lower than that in the control group on the 7th day after the intervention, and the expression of LIS1 in the cytoplasm of the high concentration intervention group was significantly lower than that of the low concentration intervention group. The results of immunocytochemical staining showed that the average optical density of LIS1 in the high concentration intervention group was significantly lower than that in the control group and the low concentration intervention group on the 7th day after the intervention of corticosterone, and the average optical density of LIS1 in the low concentration intervention group was significantly lower than that in the control group at 7 days. Conclusion corticosterone can down-regulate the expression of migration protein LIS1 in cortical neurons of developing fetus in vitro, which is related to the concentration of corticosterone and the duration of action.
【作者單位】： 中南大學(xué)湘雅二醫(yī)院兒童醫(yī)學(xué)中心;
【基金】：國(guó)家自然科學(xué)基金(81671506)
【分類(lèi)號(hào)】：R722.1

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