本文選題：產(chǎn)前給藥 + 地塞米松��； 參考：《重慶醫(yī)科大學(xué)》2012年碩士論文

【摘要】：目的：探討產(chǎn)前使用地塞米松對(duì)新生SD大鼠小腸發(fā)育及腸道Toll樣受體4（Toll-likereceptor4，TLR4）表達(dá)的影響。 方法：12只成年SD孕鼠隨機(jī)分為正常對(duì)照組、產(chǎn)前地塞米松干預(yù)組（dexamethasone，DEX組）、產(chǎn)前生理鹽水干預(yù)組（normalsaline,NS組）三組，每組4只，于孕16、17、18天時(shí)，DEX組孕鼠肌肉注射地塞米松(0.5mg/(kg·d))，NS組孕鼠肌肉注射生理鹽水(0.3ml/d)。正常對(duì)照組不予處理。各組孕鼠自然分娩（n=22天）。于第1、3、5天時(shí)處死各組新生鼠，稱量小腸重量及長度；取近回盲部腸組織做病理切片，采用HE染色觀察測量腸絨毛高度，免疫組化法檢測TLR4蛋白表達(dá)；其余小腸部分熒光實(shí)時(shí)定量PCR法檢測其TLR4mRNA的表達(dá)。 結(jié)果：（1）各組新生大鼠隨鼠齡增加，小腸長度逐漸增長，小腸重量逐漸增重，腸絨毛高度逐漸增高，小腸TLR4蛋白和mRNA表達(dá)逐漸減少。（2）1、3日齡時(shí)，DEX組新生鼠小腸長度及重量顯著高于正常對(duì)照組及NS組(P0.05)，（3）1、3、5日齡時(shí)，DEX組小腸絨毛高度顯著高于其他兩組(P0.05)，DEX組新生鼠小腸TLR4蛋白和mRNA表達(dá)顯著低于其他兩組(P0.05)。（4）正常對(duì)照組與NS組比較，各時(shí)間點(diǎn)小腸長度與重量、腸絨毛高度及腸道TLR4表達(dá)無顯著性差異(P＞0.05)。 結(jié)論：產(chǎn)前地塞米松的使用可促進(jìn)新生鼠早期小腸長度與重量的增長，增加腸絨毛高度，促進(jìn)小腸形態(tài)發(fā)育成熟；產(chǎn)前地塞米松使用可減少新生鼠腸道TLR4的表達(dá)。 目的：觀察產(chǎn)前地塞米松(dexamethasone,DEX)使用對(duì)新生大鼠壞死性小腸結(jié)腸炎（necrotizingenterocolitis，NEC）發(fā)生率及腸道Toll樣受體4（Toll-likereceptor4，TLR4）表達(dá)的影響，探討其對(duì)NEC的保護(hù)作用及可能的機(jī)制。 方法：實(shí)驗(yàn)分為正常對(duì)照組、產(chǎn)前地塞米松干預(yù)組（DEX組）、產(chǎn)前生理鹽水干預(yù)組（normalsaline,NS組）三組，于孕16、17、18天時(shí)DEX組孕鼠肌肉注射DEX(0.5mg/(kg·d))，NS組注射NS(0.3ml/d)。DEX組及NS組所生新生鼠建立NEC動(dòng)物模型。正常對(duì)照組不予處理。全部新生鼠于第5日處死，取近回盲部腸組織做病理切片，采用HE染色觀察病理變化并做評(píng)分；免疫組化法檢測各組TLR4蛋白的表達(dá)；其余小腸部分熒光實(shí)時(shí)定量PCR法檢測TLR4mRNA的表達(dá)。 結(jié)果：與NS組相比，DEX組新生鼠NEC發(fā)生率明顯降低（P0.001)。正常對(duì)照組未出現(xiàn)NEC癥狀，NS組新生鼠出現(xiàn)典型的NEC癥狀，DEX組NEC癥狀比NS組出現(xiàn)得更晚、更輕；與正常對(duì)照組相比，DEX組和NS組腸組織病理學(xué)評(píng)分及TLR4表達(dá)顯著增高（P0.05)；與NS組相比，，DEX組腸組織病理學(xué)評(píng)分及TLR4表達(dá)顯著降低(P0.05)。 結(jié)論：產(chǎn)前使用DEX對(duì)NEC大鼠具有保護(hù)作用，其機(jī)制可能是通過降低TLR4的表達(dá)從而發(fā)揮抗炎效應(yīng)。
[Abstract]:Aim: to investigate the effects of prenatal administration of dexamethasone on small intestine development and expression of Toll like receptor 4 Toll-like receptor 4 (TLR4) in neonatal SD rats.Methods Twelve adult SD pregnant rats were randomly divided into normal control group, dexamethasone DEX group and normal saline intervention group (n = 4 in each group).The pregnant rats in DEX group were intramuscularly injected with dexamethasone (0.5 mg / kg / kg) on the 18th day of gestation. The pregnant rats in NS group were intramuscularly injected with normal saline (0.3 ml / d).The normal control group was not treated.The pregnant rats in each group were given natural delivery for 22 days.The neonate rats were killed at the 3rd day of the first week to weigh the weight and length of the small intestine, the proximal ileocecal intestinal tissue was taken as pathological sections, the villi height was observed by HE staining, and the expression of TLR4 protein was detected by immunohistochemical method.The expression of TLR4mRNA in other small intestine was detected by real-time quantitative PCR.Results (1) the length of small intestine, the weight of small intestine and the height of villi increased with the age of rats.The expression of TLR4 protein and mRNA in the small intestine gradually decreased. The length and weight of small intestine in the DEX group were significantly higher than those in the normal control group and NS group at the age of 5 days. The villus height of the small intestine in the DEX group was significantly higher than that in the other two groups.The expression of white and mRNA was significantly lower than that of the other two groups.There was no significant difference in intestinal length and weight, villus height and intestinal TLR4 expression at different time points (P > 0.05).Conclusion: the use of prenatal dexamethasone can increase the length and weight of small intestine, increase the height of villi and promote the maturation of small intestine, and the use of prenatal dexamethasone can reduce the expression of TLR4 in intestine of newborn rats.Objective: to observe the effect of dexamethasone (DEX) on the incidence of necrotizing enterocolitis (NECs) and the expression of Toll-like receptor 4 (TLR4) in neonatal rats with necrotizing enterocolitis.Methods: the experiment was divided into normal control group, prenatal dexamethasone intervention group (DEX group), prenatal normal saline intervention group (NS group) three groups. At the 18th day of gestation, the pregnant rats of DEX group were injected intramuscularly with NS(0.3ml/d).DEX group and NS group to establish NEC animal model.The normal control group was not treated.All the neonate rats were killed on the 5th day. The intestinal tissues of proximal ileocecum were taken as pathological sections. The pathological changes were observed by HE staining and the expression of TLR4 protein in each group was detected by immunohistochemical method.The expression of TLR4mRNA in other small intestine was detected by real-time quantitative PCR.Results: compared with NS group, the incidence of NEC in DEX group was significantly lower than that in NS group (P 0.001).No NEC symptoms were found in the normal control group. The typical NEC symptoms were found in the newborn rats of NS group. The NEC symptoms in DEX group were later and lighter than those in NS group.Compared with the normal control group, the intestinal histopathological score and TLR4 expression in the DEX group and NS group were significantly higher than those in the NS group, and the intestinal histopathological score and TLR4 expression in the DEX group were significantly lower than those in the NS group.Conclusion: prenatal use of DEX has protective effect on NEC rats, and its mechanism may be to play an anti-inflammatory effect by reducing the expression of TLR4.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R722.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 賈盛華;韋紅;余加林;魏小娣;張曉萍;李金純;;姜黃素對(duì)新生大鼠壞死性小腸結(jié)腸炎的保護(hù)作用[J];中國當(dāng)代兒科雜志;2010年02期

2 魏小娣;韋紅;賈盛華;劉瑋;張曉萍;;姜黃素對(duì)新生大鼠壞死性小腸結(jié)腸炎模型NF-κB,TNF-α及IL-6表達(dá)的影響[J];第四軍醫(yī)大學(xué)學(xué)報(bào);2009年22期

3 李金純;韋紅;賈盛華;魏小娣;;新生兒壞死性小腸結(jié)腸炎動(dòng)物模型建立方法改進(jìn)與比較[J];重慶醫(yī)科大學(xué)學(xué)報(bào);2009年03期

本文編號(hào)：1754311