本文選題：燒傷 + 幼鼠　； 參考：《重慶醫(yī)科大學(xué)》2013年碩士論文

【摘要】：第一部分嚴(yán)重?zé)齻麑τ资笊L發(fā)育及生長激素水平影響的實(shí)驗(yàn)研究 目的 觀察幼鼠嚴(yán)重?zé)齻篌w格發(fā)育、學(xué)習(xí)記憶能力和運(yùn)動能力的改變，以及體內(nèi)生長激素水平的變化趨勢，為嚴(yán)重?zé)齻蟾深A(yù)性治療打下基礎(chǔ)。 方法 3-4周Balb/c小鼠隨機(jī)平均分成四組：空白對照組、陰性對照組、7秒燒傷組和10秒燒傷組，每組26只。燒傷前測量小鼠體重、體長、尾長，傷后1d、3d、7d時(shí)每組分別隨機(jī)抽取6只小鼠采血， ELISA方法測定血清中生長激素含量。每組剩余的8只小鼠飼養(yǎng)至成年后再次測量小鼠的體重、體長、尾長，行水迷宮實(shí)驗(yàn)（包括定位航行實(shí)驗(yàn)和空間探索實(shí)驗(yàn)）測試學(xué)習(xí)記憶能力，行負(fù)重游泳實(shí)驗(yàn)和爬桿實(shí)驗(yàn)測試運(yùn)動能力。 結(jié)果 （1）7s燒傷組和10s燒傷組小鼠的體重、體長、尾長增長百分比均落后于正常對照組（P＜0.05，，P＜0.01，P＜0.01）； （2）7s燒傷組小鼠的逃避潛伏期、跨越平臺次數(shù)及目標(biāo)現(xiàn)象時(shí)間比例與正常對照組無明顯差異；10s燒傷組的逃避潛伏期明顯長于正常對照組（P＜0.05），而空間探索實(shí)驗(yàn)示跨越平臺次數(shù)和目標(biāo)現(xiàn)象時(shí)間比例較正常對照組增加（P＜0.05， P＜0.01）。 （3）7s燒傷組小鼠爬桿時(shí)間和正常對照組相比，差異無統(tǒng)計(jì)學(xué)意義，而負(fù)重游泳時(shí)間較正常對照組縮短；10s燒傷組小鼠的爬桿時(shí)間和負(fù)重游泳時(shí)間較正常對照組縮短（P＜0.05， P＜0.01）。 （4）7s燒傷組小鼠傷后第1d、3d生長激素水平較正常對照組降低（P＜0.05），傷后7d時(shí)與正常對照組相比差異無統(tǒng)計(jì)學(xué)意義；10s燒傷組小鼠生長激素在傷后1d、3d和7d均低于對照組，（P＜0.01、P＜0.01、P＜0.05）。 結(jié)論 嚴(yán)重?zé)齻麑?dǎo)致幼鼠體格發(fā)育不良，學(xué)習(xí)記憶能力和運(yùn)動能力受到影響；同時(shí)嚴(yán)重?zé)齻笥资篌w內(nèi)生長激素水平明顯降低。 第二部分重組人生長激素對嚴(yán)重?zé)齻资蟮闹委熥饔?目的 觀察rhGH對嚴(yán)重?zé)齻资篌w格發(fā)育、學(xué)習(xí)記憶能力、運(yùn)動能力、血清白蛋白和癥因子水平等的影響。 方法 3-4周Balb/c小鼠，隨機(jī)分成6組：正常對照組組、陰性對照組、10s燒傷組、NS對照組，rhGH1mg.kg-1.d-1治療組和rhGH3mg.kg-1.d-1治療組。造模前測量各組小鼠體重、體長和尾長。正常對照組小鼠不做處理；陰性對照組小鼠麻醉、脫毛等處理同燒傷組；10s燒傷組采用如上介紹方法制作10s燒傷模型；NS對照組、rhGH1mg.kg-1.d-1治療組和rhGH3mg.kg-1.d-1治療組燒傷后每日早上8點(diǎn)分別皮下注射NS、rhGH1mg/kg和rhGH3mg/kg，持續(xù)10d。傷后1d、3d、5d隨機(jī)抽取正常對照組組、陰性對照組和10s燒傷組每組6只小鼠取血，傷后3d、5d隨機(jī)抽取NS對照組，rhGH1mg.kg-1.d-1治療組和rhGH3mg.kg-1.d-1治療組每組6只小鼠取血，剩余小鼠飼養(yǎng)至成年,成年后再次測量體重、體長、尾長，行水迷宮實(shí)驗(yàn)（包括定位航行實(shí)驗(yàn)和空間探索實(shí)驗(yàn)）測試學(xué)習(xí)記憶能力，行負(fù)重游泳實(shí)驗(yàn)和爬桿實(shí)驗(yàn)測試運(yùn)動能力。ELISA方法檢測血清IL-6和TNF-α含量，同時(shí)檢測血清中白蛋白、肌酐、葡萄糖、鈣離子水平。 結(jié)果 （1）與10s燒傷組及NS對照組相比，rhGH1mg.kg-1.d-1治療組小鼠體重、體長、尾長增長百分比增加，但差異無統(tǒng)計(jì)學(xué)意義；而rhGH3mg.kg-1.d-1治療組的體重、體長、尾長增長百分比與10s燒傷組和NS對照組相比差異有統(tǒng)計(jì)學(xué)意義（P＜0.05，P＜0.05，P＜0.01）； （2）rhGH1mg.kg-1.d-1治療組小鼠第6d逃避潛伏期較10s燒傷組和NS對照組縮短（P＜0.05），目標(biāo)現(xiàn)象停留時(shí)間延長（P＜0.01）；rhGH3mg.kg-1.d-1治療組小鼠第5d、第6d的逃避潛伏期較10s燒傷組和NS對照組均縮短（P＜0.05，P＜0.01），穿越平臺次數(shù)和目標(biāo)象限停留時(shí)間較燒傷組增多（P＜0.05，P＜0.01）； （3）rhGH3mg.kg-1.d-1治療組小鼠負(fù)重游泳時(shí)間和爬桿時(shí)間較10s燒傷組及NS對照組延長，差異有統(tǒng)計(jì)學(xué)意義（P＜0.05）; （4）小鼠燒傷后血清白蛋白水平降低（傷后1d、3d、5d P＜0.01）；血肌酐水平升高（傷后1d、3d、5d P＜0.05）；血清鈣降低（傷后1d、3d、5d P＜0.01）；血糖升高（3d、5d P＜0.05）； （5）傷后3d（治療2d后），rhGH1mg.kg-1.d-1治療組和rhGH3mg.kg-1.d-1治療組白蛋白水平升高（P＜0.05）；rhGH1mg.kg-1.d-1治療組和rhGH3mg.kg-1.d-1治療組血清肌酐降低（P＜0.05）； rhGH3mg.kg-1.d-1治療組血鈣升高（P＜0.05）；rhGH1mg.kg-1.d-1治療組和rhGH3mg.kg-1.d-1治療組血糖降低（P＜0.05）。 （6）傷后5d（治療4d后），rhGH1mg.kg-1.d-1治療組和rhGH3mg.kg-1.d-1治療組白蛋白水平升高（P＜0.05）；rhGH1mg.kg-1.d-1治療組和rhGH3mg.kg-1.d-1治療組血清肌酐降低（P＜0.05）；rhGH1mg.kg-1.d-1治療組和rhGH3mg.kg-1.d-1治療組血糖降低（P＜0.05）；治療組和燒傷組及NS對照組血鈣水平無明顯差異。 （7）與正常小鼠比較，小鼠燒傷后1d、3d和5d血清中TNF-α和IL-6水平明顯升高（P＜0.01）；rhGH1mg.kg-1.d-1治療組2d、4d時(shí)IL-6濃度較燒傷組及NS對照組降低（P＜0.05）；rhGH3mg.kg-1.d-1治療組在治療后2d、4d降低（P＜0.01，P＜0.05）。rhGH1mg.kg-1.d-1治療組和rhGH3mg.kg-1.d-1治療組小鼠TNF-α水平在治療后2d、4d與燒傷組及NS對照組無明顯差異。 結(jié)論 幼鼠嚴(yán)重?zé)齻�，rhGH通過促進(jìn)蛋白質(zhì)合成，抑制炎癥反應(yīng)等，降低嚴(yán)重?zé)齻蟮母叻纸獯x狀態(tài)及炎癥反應(yīng)狀態(tài)，從而改善嚴(yán)重?zé)齻鸬捏w格發(fā)育落后、學(xué)習(xí)記憶能力落后及運(yùn)動能力下降。
[Abstract]:Experimental study on the effects of severe burn on growth and growth hormone level in young rats
objective
We observed the changes of physical development, learning and memory ability and motor ability, and the change trend of growth hormone levels in severely burned rats, which laid the foundation for intervention treatment after severe burn.
Method
The 3-4 week Balb/c mice were randomly divided into four groups: blank control group, negative control group, 7 seconds and 10 seconds burn group and burn group, 26 rats in each group. Burn measured before the mice weight, body length, tail length, 1D after injury, 3D, 7d in each group were randomly selected 6 mice blood, ELISA method growth hormone content in serum of each group. The remaining 8 mice reared to adulthood measured again after mice body weight, body length, tail length, water maze test (including navigation experiment and space exploration experiment) to test the ability of learning and memory for swimming experiment and pole test exercise capacity.
Result
(1) the percentage of body weight, body length and tail length in the 7S burn group and the 10s burn group were all behind the normal control group (P < 0.05, P < 0.01, P < 0.01).
(2) 7S burn mice escape latency, the number of platform across time and target phenomenon ratio had no significant difference with normal control group; 10s burn group the escape latency was significantly longer than the normal control group (P < 0.05), while the space exploration experiment times of crossing the platform and target as the proportion of time increased compared with normal control group (P < 0.05, P < 0.01).
(3) there was no significant difference in the climbing time between 7S burn group and normal control group, while the time of loading swimming was shorter than that of normal control group. The time of creeping rod and weight swimming in 10s burn group was shorter than that in normal control group (P < 0.05, P < 0.01).
(4) in 7S burn group, the level of growth hormone at 1D and 3D after injury was lower than that in normal control group (P < 0.05). There was no significant difference in 7d after injury compared with that in normal control group. The growth hormone in 10s burn group was lower than that in control group after injury (P < 0.01, P < 0.01, 7d < 0.05).
conclusion
Severe burn resulted in poor physical development in young rats, and learning memory and exercise ability were affected. Meanwhile, the growth hormone level in young rats after severe burn was significantly reduced.
The therapeutic effect of recombinant human growth hormone on young rats with severe burn injury in the second part
objective
The effects of rhGH on the physical development, learning and memory ability, exercise ability, serum albumin and the level of the disease factor in young rats with severe burn were observed.
Method
The 3-4 week Balb/c mice were randomly divided into 6 groups: normal control group, negative control group, 10s burn group, NS control group, rhGH1mg.kg-1.d-1 treatment group and rhGH3mg.kg-1.d-1 treatment group. Rats measured before the mice weight, body length and tail length. Mice in normal control group without treatment; negative control group mice were anesthetized. With the hair removal and burn group; 10s group adopts the method of making the burn burn 10s model; NS control group, rhGH1mg.kg-1.d-1 treatment group and rhGH3mg.kg-1.d-1 treatment group after burn at 8 every morning were subcutaneous injections of NS, rhGH1mg/kg and rhGH3mg/kg, 10d. after injury 1D, 3D, 5D were randomly selected from the normal control group, negative control group 10s and burn group 6 mice in each group blood 3D after injury, 5D were randomly selected from the NS control group, rhGH1mg.kg-1.d-1 treatment group and rhGH3mg.kg-1.d-1 treatment group, each group of 6 mice blood, the remaining mice were reared to adult, adult To measure body weight, body length, tail length, water maze test (including navigation experiment and space exploration experiment) to test the ability of learning and memory for swimming experiment and pole test exercise capacity.ELISA method for detection of serum IL-6 and TNF- alpha content, serum albumin, creatinine, glucose, calcium level.
Result
(1) compared with 10s burn group and NS control group, rhGH1mg.kg-1.d-1 treatment group, the body weight, body length, tail length growth percentage increased, but the difference was not statistically significant; while rhGH3mg.kg-1.d-1 treatment group's body weight, body length, tail length and percentage growth compared to 10s burn group and NS control group had significant difference (P < 0.05, P < 0.05, P < 0.01);
(2) rhGH1mg.kg-1.d-1 treatment group at 6D latency than 10s burn group and NS control group (P < 0.05), shorten the residence time of target phenomenon (P < 0.01); rhGH3mg.kg-1.d-1 treatment group at 5D, the 6D latency than 10s burn group and NS control group were shorter (P < 0.05, P < 0.01), the number of cross platform and target quadrant time than burn group increased (P < 0.05, P < 0.01);
(3) the time of swimming and the time of climbing pole in the rhGH3mg.kg-1.d-1 treatment group were longer than those in the 10s group and the NS control group, and the difference was statistically significant (P < 0.05).
(4) the level of serum albumin in burned mice decreased (1D, 3D, 5D P < 0.01), serum creatinine level increased (1D after injury, 3D, 5D P < 0.05), serum calcium decreased (1D, 3D, 5D 5D < 0.01) and blood sugar increased (< 0.05).
(5) 3D after injury (2D after treatment), rhGH1mg.kg-1.d-1 treatment group and rhGH3mg.kg-1.d-1 treatment group increased albumin level (P < 0.05); rhGH1mg.kg-1.d-1 treatment group and rhGH3mg.kg-1.d-1 treatment group serum creatinine decreased (P < 0.05); rhGH3mg.kg-1.d-1 treatment group, serum calcium (P < 0.05); rhGH1mg.kg-1.d-1 treatment group and rhGH3mg.kg-1.d-1 treatment group decreased blood glucose (P < 0.05).
(6) 5D after injury (4D after treatment), rhGH1mg.kg-1.d-1 treatment group and rhGH3mg.kg-1.d-1 treatment group increased albumin level (P < 0.05); rhGH1mg.kg-1.d-1 treatment group and rhGH3mg.kg-1.d-1 treatment group serum creatinine decreased (P < 0.05); rhGH1mg.kg-1.d-1 treatment group and rhGH3mg.kg-1.d-1 treatment group decreased blood glucose (P < 0.05); treatment group and burn group NS and control group serum calcium levels were not significantly different.
(7) compared with the normal mice, mice after burn 1D, TNF- alpha and IL-6 levels of 3D and 5D in serum were significantly increased (P < 0.01); rhGH1mg.kg-1.d-1 group 2D, 4D IL-6 concentration is lower than burn group and NS control group (P < 0.05); rhGH3mg.kg-1.d-1 treatment group after treatment, 2D, 4D decreased (P < 0.01, P < 0.05) in.RhGH1mg.kg-1.d-1 group and rhGH3mg.kg-1.d-1 group were TNF- levels at 2D after treatment, no significant difference between 4D and burn group and NS control group.
conclusion
After severe burn, rhGH can reduce the state of catabolism and inflammatory reaction after severe burn by promoting protein synthesis and inhibiting inflammatory reaction, so as to improve the development of physical burn caused by severe burns, backward learning and memory ability and decreased motor ability.

【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R726.2

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