本文選題：臍血 + 端粒長(zhǎng)度��； 參考：《南京醫(yī)科大學(xué)》2016年博士論文

【摘要】：端粒(Telomere)是特異結(jié)合蛋白與真核細(xì)胞染色體末端的TTAGGG六個(gè)堿基重復(fù)序列的復(fù)合體,在維持染色體的完整性和基因組穩(wěn)定性方面具有極其重要的作用。隨著細(xì)胞分裂,端粒進(jìn)行性縮短,端粒長(zhǎng)度可以反映機(jī)體在細(xì)胞水平的衰老程度,目前許多研究證實(shí)成人端粒長(zhǎng)度可以作為年齡相關(guān)疾病的風(fēng)險(xiǎn)標(biāo)志物。雖然組織來(lái)源的細(xì)胞能夠?yàn)檠芯慷肆ｉL(zhǎng)度與疾病關(guān)系提供更直接的證據(jù),但由于不同組織間端粒長(zhǎng)度存在高度的相關(guān)性,外周血樣本產(chǎn)生DNA質(zhì)量高且更加容易獲取,因此目前外周血被廣泛用于端粒長(zhǎng)度的研究中。流行病學(xué)研究發(fā)現(xiàn)成人的端粒長(zhǎng)度主要受到環(huán)境因素和遺傳因素的共同影響,端粒長(zhǎng)度取決于出生時(shí)端粒長(zhǎng)度及出生后氧化應(yīng)激程度。不同個(gè)體出生時(shí)端粒長(zhǎng)度有明顯差異,且研究發(fā)現(xiàn)生命早期端粒長(zhǎng)度比生命后期端粒長(zhǎng)度能夠更好地預(yù)測(cè)壽命并且能對(duì)個(gè)體產(chǎn)生終身的影響,其影響程度超過生命后期的其他風(fēng)險(xiǎn)暴露,因此研究新生兒端粒長(zhǎng)度具有重要的意義。但目前新生兒端粒長(zhǎng)度的研究樣本量較小,影響因素不確切,研究結(jié)果也不一致。另外研究證實(shí)新生兒端粒長(zhǎng)度與遺傳因素相關(guān),但目前國(guó)內(nèi)外僅有研究成人端粒長(zhǎng)度的遺傳變異,新生兒端粒長(zhǎng)度與遺傳變異的研究尚未見報(bào)道。因此,本研究在第一部分主要探索環(huán)境因素對(duì)新生兒端粒長(zhǎng)度的影響;第二部分主要研究遺傳因素對(duì)新生兒端粒長(zhǎng)度的影響。第一部分:環(huán)境因素對(duì)新生兒端粒長(zhǎng)度的影響流行病學(xué)研究已經(jīng)對(duì)新生兒端粒長(zhǎng)度的社會(huì)人口學(xué)因素及環(huán)境暴露因素進(jìn)行了探索,Friedrich等對(duì)15例新生兒的研究發(fā)現(xiàn),孕周、新生兒出生體重等都影響臍血端粒長(zhǎng)度,Entringer等人對(duì)27例新生兒的研究發(fā)現(xiàn),孕期的精神壓力也顯著影響臍血端粒長(zhǎng)度,而Cross JA等在81例新生兒中的研究發(fā)現(xiàn),臍血白細(xì)胞端粒長(zhǎng)度與母親年齡、孕周、吸煙、新生兒出生體重等均無(wú)明顯相關(guān)關(guān)系。因此,目前已有的研究不僅樣本量較小,而且研究結(jié)論尚不一致。本研究采用橫斷面研究深入分析影響新生兒臍靜脈血相對(duì)端粒長(zhǎng)度的相關(guān)環(huán)境因素。研究對(duì)象為南京鼓樓醫(yī)院出生的444例新生兒,對(duì)新生兒母親進(jìn)行問卷調(diào)查、體格檢查和臨床相關(guān)指標(biāo)檢測(cè),采集新生兒臍靜脈血及與其配對(duì)的母體外周血樣本。采用定量PCR方法用特異性引物將待測(cè)基因組的端粒(Telomere,T)序列和單拷貝基因(Single copy gene,S)序列同時(shí)進(jìn)行擴(kuò)增,用二者拷貝數(shù)的比值(T/S)來(lái)定量相對(duì)端粒長(zhǎng)度(Relative telomere length,RTL)。為保證端粒長(zhǎng)度檢測(cè)的準(zhǔn)確性,實(shí)驗(yàn)操作中進(jìn)行復(fù)孔平行設(shè)置和重復(fù)檢測(cè)等質(zhì)量控制措施。采用配對(duì)T檢驗(yàn)進(jìn)行母兒端粒長(zhǎng)度之間的比較,采用方差分析和t檢驗(yàn)進(jìn)行不同組間相對(duì)端粒長(zhǎng)度差異的比較;采用一般線性模型分析新生兒端粒長(zhǎng)度與母親端粒長(zhǎng)度等連續(xù)性變量之間的相關(guān)性。研究結(jié)果顯示,新生兒端粒長(zhǎng)度(均數(shù)為0.358)顯著大于母親端粒長(zhǎng)度(均數(shù)為0.309)(P0.001),未發(fā)現(xiàn)母兒端粒長(zhǎng)度之間有明顯相關(guān)關(guān)系(R2 = 0.004,P=0.174)。母親受教育程度越高,新生兒端粒長(zhǎng)度越長(zhǎng)(P=0.022)。孕周、分娩方式、新生兒性別、出生體重與母兒端粒長(zhǎng)度均未見明顯關(guān)聯(lián)(P0.05)。孕期攝入奶制品較多,母親端粒長(zhǎng)度較短(P=0.048),孕期蔬菜水果、肉制品、動(dòng)物內(nèi)臟、魚蝦、豆制品、煎烤及腌制食品、葉酸以及多種維生素?cái)z入不同組之間,新生兒和母親端粒長(zhǎng)度均未發(fā)現(xiàn)顯著差異(P0.05)。孕期飲用咖啡新生兒端粒長(zhǎng)度短(P=0.011)。孕期二手煙暴露、飲酒、飲茶情況對(duì)母親和新生兒端粒長(zhǎng)度未見明顯影響(P0.05)。孕期經(jīng)常參加體育鍛煉的孕婦端粒長(zhǎng)度長(zhǎng)于不鍛煉者或者很少鍛煉者(P=0.01),而新生兒端粒長(zhǎng)度未見顯著差異(P=0.187)。本研究較全面評(píng)估了孕期的重要環(huán)境因素對(duì)孕婦及新生兒端粒長(zhǎng)度的影響,研究結(jié)果將為揭示端粒在疾病發(fā)生中的作用提供了重要線索。第二部分:遺傳因素對(duì)新生兒端粒長(zhǎng)度的影響研究表明,遺傳因素能夠解釋36%～90%的個(gè)體之間端粒長(zhǎng)度的變異,因此,研究影響端粒長(zhǎng)度的遺傳變異位點(diǎn)和基因?qū)τ陉U明端粒長(zhǎng)度受遺傳調(diào)控的內(nèi)在機(jī)制具有十分重要的意義。全基因組關(guān)聯(lián)研究(Genome-wide association study,GWAS)方法近年被廣泛應(yīng)用于基因與疾病的關(guān)聯(lián)研究,其通過對(duì)大規(guī)模的群體DNA樣本進(jìn)行全基因組高密度遺傳標(biāo)記分型,從而尋找與復(fù)雜疾病相關(guān)的遺傳因素的研究方法,全面揭示疾病發(fā)生、發(fā)展與治療相關(guān)的遺傳基因。目前為止,基于歐洲人群的GWAS研究發(fā)現(xiàn)有多個(gè)遺傳變異位點(diǎn)與端粒長(zhǎng)度相關(guān),其中包括3q26(TERC)、4q32.2(NAF1)、5p15.33(TERT)、10q24.33(OBFC1)和 20q13.3(RTEL1)等這些已經(jīng)被研究證明直接參與端粒生物學(xué)功能調(diào)控的基因的染色體區(qū)域[37-41],也包含2p16.2(ACYP2)和19p12(ZNF208)等還沒有相關(guān)證據(jù)證明其參與端粒生物學(xué)調(diào)控的基因的染色體區(qū)域。這些研究發(fā)現(xiàn)為闡明影響端粒長(zhǎng)度的遺傳機(jī)制提供重要線索。但是,這些研究均基于成年人,其端粒長(zhǎng)度既與生命早期的端粒長(zhǎng)度有關(guān),也受到生長(zhǎng)過程中環(huán)境暴露導(dǎo)致的端�？s短速度影響。然而,目前關(guān)于新生兒端粒長(zhǎng)度相關(guān)遺傳變異的研究尚未見報(bào)道。本研究利用TaqMan 7900基因分型平臺(tái)在444例臍靜脈血及與其對(duì)應(yīng)的母血中對(duì)目前GWAS已報(bào)道端粒長(zhǎng)度相關(guān)位點(diǎn)進(jìn)行研究。為了更加全面的分析既往GWAS報(bào)道端粒長(zhǎng)度相關(guān)遺傳變異與母兒端粒長(zhǎng)度的相關(guān)性,本研究對(duì)這8個(gè)遺傳變異位點(diǎn)的基因型進(jìn)行加權(quán)遺傳評(píng)分(Weighted genetic score,WGS)的計(jì)算,分析WGS與母體外周血及臍靜脈血端粒長(zhǎng)度的相關(guān)關(guān)系,評(píng)價(jià)8個(gè)遺傳變異與母兒端粒長(zhǎng)度的綜合效應(yīng)。遺傳變異及加權(quán)遺傳評(píng)分與端粒長(zhǎng)度的相關(guān)性采用一般線性模型分析。研究結(jié)果顯示,歐洲人群端粒長(zhǎng)度顯著相關(guān)的遺傳變異rs10936599和rs2736100與中國(guó)孕婦端粒長(zhǎng)度顯著相關(guān)(β=0.0119,P=00.41及β=0.011,P=0.046),已知的8個(gè)遺傳變異的加權(quán)效應(yīng)與母親端粒長(zhǎng)度的相關(guān)性更強(qiáng)(R2=0.012,P=0.025);本研究發(fā)現(xiàn),上述8個(gè)遺傳變異單獨(dú)或聯(lián)合與新生兒端粒長(zhǎng)度均不存在顯著關(guān)聯(lián)(P=0.716)。本研究首次發(fā)現(xiàn)已知端粒長(zhǎng)度相關(guān)的遺傳變異可能不影響新生兒的端粒長(zhǎng)度,但影響成長(zhǎng)過程中不同個(gè)體對(duì)環(huán)境暴露導(dǎo)致端�？s短的易感程度。本研究首次分析了遺傳變異與新生兒及與孕母端粒長(zhǎng)度的相關(guān)關(guān)系,為探索端粒長(zhǎng)度遺傳調(diào)控機(jī)制研究提供了重要線索。
[Abstract]:Telomere length (Telomere) is a complex of specific binding protein and the ends of eukaryotic chromosomes TTAGGG six nucleotide repeats, plays an important role in maintaining chromosome integrity and genomic stability. With cell division, telomere shortening of telomere length, can reflect the aging degree of the body at the cellular level, at present a lot of research that telomere length can be used as a marker of risk of adult age related diseases. Although the cells can provide more direct evidence for the research on the relationship between telomere length and disease, but due to the different interstitial telomere length has high correlation with peripheral blood samples of DNA have high quality and more easily accessible, so the peripheral blood was widely used in the study of telomere length in adult. Epidemiological studies have found that telomere length is mainly affected by environmental factors and genetic factors. The combined effect on telomere length in telomere length and after birth when the degree of oxidative stress. Different individuals born when telomere length were significantly different, and the study found that early life telomere length of telomere length in later life to be able to better predict the life and production of individual lifelong effects, the effects of other risk more than later in life so the study of neonatal exposure, telomere length has important significance. But at present the research sample of neonatal telomere length is smaller, the influence factors is not exact, the results are not consistent. Other studies confirmed neonatal telomere length and genetic factors related to genetic variation, but the research at home and abroad only adult telomere length and neonatal telomere length and genetic variation has not been reported. Therefore, this study in the first part mainly explores the environmental factors on neonatal telomere length The second part mainly studies the influence; influence of genetic factors on neonatal telomere length. The first part: the epidemiological study on the effects of environmental factors on neonatal telomere length has social and demographic factors on neonatal telomere length and environmental exposure factors were explored, a study of 15 cases of neonatal Friedrich, gestational age, birth weight and so on telomere length of umbilical cord blood of 27 neonates, Entringer et al found that pregnancy stress has a significant influence on telomere length and umbilical cord blood, in 81 cases of neonatal Cross JA found that umbilical cord blood leukocyte telomere length and maternal age, gestational age, smoking, there was no obvious correlation between the birth weight of newborns. Therefore, the present study not only the small sample, but the conclusion is not consistent. This study used a cross-sectional study in-depth analysis of the impact of neonatal umbilical The relative telomere length of venous blood related environmental factors. The research object for 444 neonates born in Nanjing Gulou Hospital, conducted a questionnaire survey of mothers, physical examination and detection of clinically relevant indicators, collecting umbilical cord blood and maternal matched peripheral blood samples. Using quantitative PCR method to test the genome by using specific primers of the telomere (Telomere, T) sequence and single copy gene (Single copy gene, S) sequence were amplified with two copy number ratio (T/S) to quantify the relative telomere length (Relative telomere, length, RTL). In order to ensure the accuracy of detection of telomere length, control measures and holes arranged in parallel repetition detection quality experimental operation. Paired T test was used for comparison between the telomere length of mother and infant, compared with analysis of variance and t test were different between group differences in the relative telomere length; The general linear model to analyze the correlation between neonatal telomere length and mother telomere length continuity variables. The results showed that the telomere length of newborn (mean 0.358) was significantly higher than the mother of telomere length (mean 0.309) (P0.001), there was no obvious correlation between maternal telomere length (R2 = 0.004. P=0.174). The higher the education degree of mother, neonatal telomere length (P=0.022). The longer the gestational age, mode of delivery, neonatal sex, birth weight and maternal telomere length had no significant correlation (P0.05). During the intake of dairy products more, mother short telomere length (P=0.048) during pregnancy, fruits and vegetables, meat products, animal offal, fish, beans, roasted and salted food, folic acid and vitamins intake between different groups, no difference was found between the newborn and mother telomere length (P0.05) during pregnancy. Drinking coffee (short telomere length in neonates P=0.011). Prenatal exposure to secondhand smoke, drinking tea, the effects on maternal and neonatal telomere length was not obvious (P0.05) during pregnancy. The pregnant women often take part in physical exercise is longer than the telomere length do not exercise or rarely exercise (P=0.01), and neonatal telomere length have no significant difference (P=0.187). This study is a comprehensive assessment of the impact the important environmental factors of pregnancy on maternal and neonatal telomere length, the results will reveal the role of telomeres in the disease provides an important clue. The second part: study the influence of genetic factors on neonatal telomere length showed that genetic factors could explain 36% to telomere length variation between 90% individuals so the effect of telomere the length of the genetic mutations and gene has very important significance to elucidate the mechanism underlying telomere length by genetic manipulation. A genome-wide association study (Geno Me-wide association study, GWAS) related research methods in recent years has been widely used in gene and disease, which based on the large-scale group DNA samples of whole genome of high density genetic marker type, in order to find out the research methods of genetic factors associated with complex diseases, fully reveal the disease occurrence, development and treatment of related genes. So far, GWAS of European populations found multiple genetic mutations associated with telomere length based on, including 3q26 (TERC), 4q32.2 (NAF1), 5p15.33 (TERT), 10q24.33 (OBFC1) and 20q13.3 (RTEL1) etc. These have been studied that chromosomal region [37-41] directly involved in telomere biology function gene also, including 2p16.2 (ACYP2) and 19p12 (ZNF208) is not related to evidence of chromosomal regions in telomere biology gene. These findings in order to elucidate the effect Provide important clues to the genetic mechanism of telomere length. However, these studies are based on the adults, the telomere length of telomere length and early life, but also by the growth process of environmental exposure leads to telomere shortening speed. However, the current research on the length of the genetic variants associated with neonatal telomere has not been reported. This study uses TaqMan 7900 genotyping platforms in 444 cases of umbilical cord blood and maternal blood in corresponding research on the current GWAS has been reported to related sites. Correlation between telomere length in order to more comprehensive analysis of the previous GWAS reports related to telomere length and the genetic variation of maternal telomere length, weighted score of the genetic genotype on the 8 the genetic mutation (Weighted genetic, score, WGS) calculations, analysis of the relationship between WGS and maternal peripheral blood and umbilical vein blood in telomere length and 8 genetic evaluation The comprehensive effect of variation and maternal telomere length. Genetic variation and genetic correlation weighted score and telomere length by using the general linear model analysis. The results showed that the correlation was significantly related to telomere length in European populations and genetic variation of rs10936599 and rs2736100 in pregnant women China telomere length (beta =0.0119, P=00.41 and beta =0.011, P=0.046), more relevant the 8 known genetic variation and mother weighting effect of telomere length (R2=0.012, P=0.025); the study found that the 8 genetic variants alone or in combination with the telomere length of newborns does not exist significant association (P=0.716). This is the first study found that genetic variation associated with telomere length may not be known to affect neonatal telomere length, but susceptible to different individual environmental exposure leads to telomere shortening of the growth process. This is the first study to analyze the genetic variability and neonatal and The correlation with the telomere length of pregnant women provides an important clue for the study of the genetic regulation mechanism of telomere length.

【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2016
【分類號(hào)】：R722.1

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 馬蘭;王晶;江濱;劉艷榮;張波;常乃柏;克曉燕;;慢性淋巴細(xì)胞白血病患者單個(gè)核細(xì)胞端粒長(zhǎng)度與端粒酶表達(dá)活性研究[J];中國(guó)實(shí)驗(yàn)血液學(xué)雜志;2009年06期

2 張寅,平飛云,張行;30例口腔頜面部惡性腫瘤端粒長(zhǎng)度變化的研究[J];口腔頜面外科雜志;2002年04期

3 郭令軍;張七一;;實(shí)時(shí)定量PCR分析外周血白細(xì)胞的端粒長(zhǎng)度[J];中外醫(yī)療;2008年21期

4 馬曉彩;孫婉玲;;血液系統(tǒng)疾病端粒長(zhǎng)度研究進(jìn)展[J];北京醫(yī)學(xué);2013年10期

5 劉利,孫秉中,梁英民,郝淼旺,鄧中榮,張傳山,閻嚴(yán),張方信;急性髓系白血病細(xì)胞端粒酶活性及端粒長(zhǎng)度的同步分析[J];中華血液學(xué)雜志;2001年11期

6 姜英華,黃迪南,祝其鋒;端粒長(zhǎng)度調(diào)控機(jī)制的研究進(jìn)展[J];腫瘤防治雜志;2004年01期

7 劉義;漢麗梅;潘永榮;金勇;;端粒長(zhǎng)度測(cè)量方法的研究進(jìn)展[J];世界華人消化雜志;2007年28期

8 林里;李濤平;;阻塞性睡眠呼吸暫停綜合征患者端粒長(zhǎng)度的變化及意義[J];南方醫(yī)科大學(xué)學(xué)報(bào);2011年03期

9 商青青;周建業(yè);胡盛壽;;端粒長(zhǎng)度的影響因素及其與心血管疾病的關(guān)系[J];中國(guó)分子心臟病學(xué)雜志;2012年02期

10 譚善娟;王娜;王威;吳擁軍;吳逸明;;人外周血基因組DNA端粒長(zhǎng)度與肺癌關(guān)系[J];中國(guó)公共衛(wèi)生;2012年05期

相關(guān)會(huì)議論文 前8條

1 呂昆;林丹;許淼;朱怡文;黃英;;應(yīng)用印跡法測(cè)定轉(zhuǎn)基因克隆牛體細(xì)胞端粒長(zhǎng)度[A];遺傳學(xué)與社會(huì)可持續(xù)發(fā)展——2010中國(guó)青年遺傳學(xué)家論壇論文摘要匯編[C];2010年

2 郝大程;李明霞;肖培根;張宇;陳瑋琦;;定量細(xì)胞端粒長(zhǎng)度的實(shí)時(shí)定量PCR方法(英文)[A];2011年中國(guó)藥學(xué)大會(huì)暨第11屆中國(guó)藥師周論文集[C];2011年

3 葉靜;Christelle Lenain;Serge Bauwen;Arturo Londono;Marie-Joseph Giraud-Panis;吳云林;Eric Gilson;;端粒長(zhǎng)度、端粒酶及其相關(guān)蛋白復(fù)合體(Telosome/Shelterin)在胃癌發(fā)生及演進(jìn)中的研究[A];第二十一屆全國(guó)中西醫(yī)結(jié)合消化系統(tǒng)疾病學(xué)術(shù)會(huì)議暨國(guó)家級(jí)中西醫(yī)結(jié)合消化系統(tǒng)疾病新進(jìn)展學(xué)習(xí)班論文匯編[C];2009年

4 劉瓊;胡德聰;王紅;丁朝建;姜亮;陶德定;徐輝碧;;微量元素鋅、鐵、鉻和鉛對(duì)細(xì)胞端粒長(zhǎng)度作用的研究[A];中國(guó)營(yíng)養(yǎng)學(xué)會(huì)第八屆微量元素營(yíng)養(yǎng)學(xué)術(shù)會(huì)議論文摘要匯編[C];2003年

5 劉劍侖;葛蓮英;黎丹戎;張貴年;;大腸癌端粒長(zhǎng)度變化與端粒酶活性的研究[A];西部地區(qū)腫瘤學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2004年

6 鞏艷;房殿春;張汝鋼;楊仕明;汪榮泉;彭貴勇;陳文生;羅元輝;;端粒植入對(duì)胃癌7901細(xì)胞生長(zhǎng)、端粒長(zhǎng)度和端粒酶活性和hTERT mRNA表達(dá)的影響[A];中華醫(yī)學(xué)會(huì)第七次全國(guó)消化病學(xué)術(shù)會(huì)議論文匯編（下冊(cè)）[C];2007年

7 詹昱;易正山;宋蘭林;馮茹;;FLOW-FISH同時(shí)檢測(cè)細(xì)胞端粒長(zhǎng)度及表面分化抗原方法的建立與優(yōu)化[A];第12屆全國(guó)實(shí)驗(yàn)血液學(xué)會(huì)議論文摘要[C];2009年

8 董矜;田亞平;;光照核黃素對(duì)腫瘤細(xì)胞生長(zhǎng)和端粒長(zhǎng)度的作用[A];第6次全國(guó)微生物學(xué)與免疫學(xué)大會(huì)論文摘要匯編[C];2004年

相關(guān)重要報(bào)紙文章 前3條

1 新汶;端粒長(zhǎng)度較短可預(yù)測(cè)卒中后的高癡呆發(fā)生風(fēng)險(xiǎn)[N];醫(yī)藥經(jīng)濟(jì)報(bào);2006年

2 王增;壽命長(zhǎng)短可能遺傳于父親[N];衛(wèi)生與生活報(bào);2007年

3 清瀾;科學(xué)家真能倒撥生命時(shí)鐘？[N];北京科技報(bào);2000年

相關(guān)博士學(xué)位論文 前5條

1 曹嵐;端粒的遺傳學(xué)分析及其與肺癌的相關(guān)性研究[D];復(fù)旦大學(xué);2014年

2 翁僑;新生兒端粒長(zhǎng)度的影響因素研究[D];南京醫(yī)科大學(xué);2016年

3 賓萍;多環(huán)芳烴暴露對(duì)端粒長(zhǎng)度的影響及易感性生物標(biāo)志研究[D];中國(guó)疾病預(yù)防控制中心;2008年

4 申載薰;芪蓮舒痞顆粒影響端粒酶活性、端粒長(zhǎng)度、DNA含量及NF-κB表達(dá)的實(shí)驗(yàn)研究[D];山東中醫(yī)藥大學(xué);2011年

5 賈文文;人端粒酶在小鼠體內(nèi)和iPS細(xì)胞中的表達(dá)調(diào)控[D];西北農(nóng)林科技大學(xué);2011年

相關(guān)碩士學(xué)位論文 前10條

1 趙菲菲;精子端粒長(zhǎng)度與IVF過程中早期胚胎發(fā)育的相關(guān)研究[D];鄭州大學(xué);2015年

2 王晗;葉酸對(duì)人BJ及A375細(xì)胞株端粒長(zhǎng)度和染色體不穩(wěn)定性的影響[D];云南師范大學(xué);2015年

3 馬杰;健康人群外周血白細(xì)胞端粒長(zhǎng)度與腎功能關(guān)系的縱向研究[D];吉林大學(xué);2016年

4 于嬌嬌;小鼠鐵過載模型的建立及其對(duì)細(xì)胞端粒長(zhǎng)度的影響[D];首都醫(yī)科大學(xué);2016年

5 童秋玲;海洛因或冰毒成癮者外周血白細(xì)胞端粒長(zhǎng)度與吸毒年限、中醫(yī)證素關(guān)系的研究[D];福建中醫(yī)藥大學(xué);2016年

6 李海菲;再生障礙性貧血中醫(yī)證型與外周血端長(zhǎng)度及TRF1，，TRF2，POT1基因表達(dá)水平的相關(guān)性研究[D];山東中醫(yī)藥大學(xué);2016年

7 白銳;男性超重/肥胖對(duì)IVF妊娠結(jié)局影響及其可能的研究機(jī)制[D];鄭州大學(xué);2016年

8 葉聰瑩;水稻端粒長(zhǎng)度相關(guān)QTL精細(xì)定位[D];浙江理工大學(xué);2016年

9 殷華宗;基于轉(zhuǎn)錄組測(cè)序數(shù)據(jù)和端粒長(zhǎng)度變化分析烏龜長(zhǎng)壽與端粒長(zhǎng)度維持的關(guān)系[D];安徽師范大學(xué);2016年

10 楊瑜;2型糖尿病周圍神經(jīng)病變經(jīng)抗氧化治療前后端粒長(zhǎng)度及端粒酶活性的變化[D];內(nèi)蒙古科技大學(xué)包頭醫(yī)學(xué)院;2015年

本文編號(hào)：1734706