本文選題：嬰幼兒喘息性疾病　切入點：淋巴細(xì)胞功能相關(guān)抗原-1　出處：《鄭州大學(xué)》2012年碩士論文

【摘要】：嬰幼兒喘息性疾病是目前最常見的嚴(yán)重危害嬰幼兒健康的呼吸道感染疾病,是由多種細(xì)胞及細(xì)胞組分參與形成的氣道炎癥反應(yīng),進(jìn)而導(dǎo)致氣道高反應(yīng)的發(fā)生。目前其病因、具體發(fā)病機(jī)制尚未完全明確。近年來,隨著分子生物學(xué)研究技術(shù)的不斷進(jìn)步,逐漸認(rèn)識到粘附分子在多種免疫應(yīng)答和炎癥性疾病致病過程中發(fā)揮著重要作用。本實驗通過檢測嬰幼兒喘息性疾病淋巴細(xì)胞功能相關(guān)抗原-1(Lymphocyte Function-associated Antigen-1, LFA-1即CD11a/CD18)和巨噬細(xì)胞分化抗原-1(Macrophages differentiation antigen-1, Mac-1即CD11b/CD18)在外周血單個核細(xì)胞表面的表達(dá)情況,并研究兩者之間的相關(guān)性,來探討LFA-1、Mac-1在嬰幼兒喘息性疾病發(fā)病過程中的作用機(jī)制,為進(jìn)一步了解嬰幼兒喘息性疾病的發(fā)病機(jī)制提供相應(yīng)的理論依據(jù)和新的思路。 目的 本實驗通過流式細(xì)胞儀檢測嬰幼兒喘息性疾病患兒LFA-1、Mac-1在外周血單個核細(xì)胞表面的表達(dá)情況,探討兩者在嬰幼兒喘息性疾病發(fā)病過程中的作用機(jī)制。 材料與方法 1研究對象及分組 研究對象為存在呼吸道感染同時伴有喘息癥狀的患兒29例,存在呼吸道感染同時不伴有喘息癥狀的患兒26例,正常對照組25例,共分為喘息組、非喘息組及對照組三組。納入本實驗的所有嬰幼兒采血前4周均無免疫藥物治療史；喘息組排除其它引起喘息的疾病,非喘息組及對照組兒童既往無喘息病史、個人或家族過敏性疾病史以及其他變態(tài)反應(yīng)性疾病史。三組嬰幼兒的年齡、性別構(gòu)成差異無統(tǒng)計學(xué)意義。 2標(biāo)本采集 各組嬰幼兒均于入院及體檢當(dāng)天采靜脈血2m1,經(jīng)EDTA-2N a抗凝后待用。 3實驗方法 采用流式細(xì)胞儀在1天內(nèi)檢測新鮮抗凝全血的LFA-1(CDlla+/CD18+). Mac-1(CDllb+/CD18+)的表達(dá)率。 4統(tǒng)計學(xué)方法 用SPSS17.0統(tǒng)計軟件包進(jìn)行統(tǒng)計學(xué)分析,所有實驗數(shù)據(jù)以均數(shù)±標(biāo)準(zhǔn)差(x±s)表示,三組間的比較采用單因素方差分析,兩兩比較采用LSD-t方法檢驗,兩個因素相關(guān)性分析采用(?) Pearson(?)(?)關(guān)性檢驗,以P0.05為差異具有統(tǒng)計學(xué)意義。 結(jié)果 1LFA-1結(jié)果 喘息組LFA-1表達(dá)率(47.46±13.94%)高于非喘息組(35.88±12.64%,P0.01)及對照組(26.62±10.41%,P0.01),非喘息組LFA-1表達(dá)率高于對照組(P0.01)。 2Mac-1結(jié)果 喘息組Mac-1表達(dá)率(7.43±3.42%)高于非喘息組(4.53±3.04%,P0.01)及對照組(2.92±1.80%,P0.01),非喘息組Mac-1表達(dá)率高于對照組(P0.05)。 3相關(guān)性分析 LFA-1與Mac-1的表達(dá)率在喘息組呈正相關(guān)(r=0.403,P0.05),而LFA-1與Mac-1的表達(dá)率在非喘息組、對照組無相關(guān)性。 結(jié)論 1.LFA-1和Mac-1的異常表達(dá)參與了嬰幼兒喘息性疾病的發(fā)病過程。 2.LFA-1和Mac-1在嬰幼兒喘息性疾病的發(fā)病過程中具有協(xié)同作用。
[Abstract]:Infant wheezing disease is the most common respiratory tract infection that seriously endangers the health of infants and young children. It is an inflammatory reaction of airway formed by many kinds of cells and cell components, which leads to the occurrence of airway hyperresponsiveness. The specific pathogenesis is not completely clear. In recent years, with the development of molecular biology research technology, It is gradually recognized that adhesion molecules play an important role in the pathogenesis of multiple immune responses and inflammatory diseases. In this study, we detected lymphocyte function associated antigen-1 Lymphocyte Function-associated antigen-1 (LFA-1, CD11a / CD18) and macrophage in infant asthmatic disease. The expression of CD11b / CD18 on the surface of peripheral blood mononuclear cells (PBMC), and the expression of CD11b / CD18, the differentiation antigen of Macrophages differentiation antigen-1, or CD11b / CD18. The relationship between LFA-1 and Mac-1 was studied to explore the mechanism of LFA-1 Mac-1 in the pathogenesis of infant wheezing disease, and to provide a theoretical basis and a new way of thinking for further understanding the pathogenesis of infant wheezing disease. Purpose. The expression of LFA-1 Mac-1 on peripheral blood mononuclear cells (PBMC) in children with wheezing diseases was detected by flow cytometry to explore the mechanism of LFA-1 Mac-1 in the pathogenesis of wheezing diseases in infants. Materials and methods. 1. Research objects and groups. The subjects were 29 children with respiratory tract infection and wheezing symptoms, 26 children with respiratory tract infection and no wheezing symptoms, and 25 normal controls, who were divided into wheezing group. No history of immunotherapy was found in all infants and infants who were enrolled in the study before taking blood samples 4 weeks before, while other diseases causing wheezing were excluded in the wheezing group, and there was no previous history of wheezing in the non-wheezing group and the control group. There was no significant difference in age and sex composition among the three groups. 2 specimen collection. Venous blood was taken from 2 ml on admission and physical examination in each group, and was treated with EDTA-2N a anticoagulant. 3 experimental method. Flow cytometry was used to detect the expression rate of LFA-1(CDlla / CD18 / Mac-1(CDllb / CD18 in fresh anticoagulant whole blood. 4 Statistical method. All the experimental data were expressed as mean 鹵standard deviation x 鹵s. The comparison among the three groups was conducted by single factor analysis of variance (ANOVA). The LSD-t method was used to test the comparison between the two groups, and the correlation analysis of the two factors was used to analyze the correlation between the two factors. (Pearsonian? Cynthia? ) correlation test, with P0.05 as the difference has statistical significance. Results. 1LFA-1 results. The expression rate of LFA-1 in asthmatic group (47.46 鹵13.94) was higher than that in non-asthmatic group (35.88 鹵12.64) and control group (26.62 鹵10.41). The expression rate of LFA-1 in non-asthmatic group was higher than that in control group (P 0.01). 2Mac-1 results. The expression rate of Mac-1 in asthmatic group (7.43 鹵3.42) was higher than that in non-asthmatic group (4.53 鹵3.04P0.01) and control group (2.92 鹵1.80P0.01). The expression rate of Mac-1 in non-asthmatic group was higher than that in control group. 3 correlation analysis. There was a positive correlation between the expression of LFA-1 and Mac-1 in wheezing group, but no correlation between LFA-1 and Mac-1 in non-asthmatic group. Conclusion. Abnormal expression of 1.LFA-1 and Mac-1 is involved in the pathogenesis of infant wheezing disease. 2.LFA-1 and Mac-1 have synergistic effect in the pathogenesis of infant wheezing disease.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R725.6

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