本文選題：急性淋巴細(xì)胞性白血病　切入點(diǎn)：p蛋白表達(dá)　出處：《南京醫(yī)科大學(xué)學(xué)報(bào)(自然科學(xué)版)》2017年04期

【摘要】：目的:探討兒童急性淋巴細(xì)胞白血病p16蛋白表達(dá)及DNA含量的臨床意義。方法:急性淋巴細(xì)胞白血病患兒58例作為觀察組,健康兒童58例作為對照組,采用SP免疫組化法檢測骨髓液中p16蛋白的表達(dá)水平;流式細(xì)胞術(shù)檢測骨髓中白血病細(xì)胞DNA的含量,比較兩組研究對象的差異。結(jié)果:觀察組患兒p16陽性表達(dá)率為37.93%,明顯低于對照組的100.00%(P0.05);觀察組患兒p16陽性表達(dá)率與年齡、性別無明顯關(guān)系(P0.05),但肝腫大4 cm患兒的p16陽性表達(dá)率明顯高于≥4 cm的患兒(P0.05),高白細(xì)胞計(jì)數(shù)與高危急性淋巴細(xì)胞白血病(HR-ALL)患兒的p16陽性表達(dá)率明顯高于低白細(xì)胞計(jì)數(shù)與標(biāo)危急性淋巴細(xì)胞白血病(SR-ALL)患兒(P0.05)。在觀察組p16蛋白陰性表達(dá)患兒中DNA異倍體發(fā)生率(66.67%)明顯高于陽性表達(dá)患兒中DNA異倍體發(fā)生率(40.90%,P0.05)。結(jié)論:p16蛋白在急性淋巴細(xì)胞白血病患兒的發(fā)病過程中起到重要作用,p16蛋白缺失可能與急性淋巴細(xì)胞白血病復(fù)發(fā)相關(guān),臨床高危因素與p16陰性表達(dá)者DNA異倍體發(fā)生率升高具有相關(guān)性。
[Abstract]:Objective: to investigate the clinical significance of p16 protein expression and DNA content in children with acute lymphoblastic leukemia. Methods: 58 children with acute lymphoblastic leukemia were treated as observation group and 58 healthy children as control group. The expression of p16 protein in bone marrow fluid was detected by SP immunohistochemical method, and the DNA content of leukemic cells in bone marrow was detected by flow cytometry. Results: the positive expression rate of p16 in the observation group was 37.93, which was significantly lower than that in the control group, and the positive expression rate of p16 in the observation group was higher than that in the control group. The positive expression rate of p16 in children with hepatomegaly 4 cm was significantly higher than that in children with liver enlargement 鈮，

本文編號：1677011