本文選題：急性呼吸道感染　切入點(diǎn)：腺病毒(AdV)　出處：《病毒學(xué)報(bào)》2017年06期

【摘要】：探討2016年12月深圳市光明新區(qū)某小學(xué)一起急性呼吸道病毒感染暴發(fā)疫情的流行病學(xué)及病原學(xué)特點(diǎn)。采用相應(yīng)的流行病學(xué)個(gè)案調(diào)查表對(duì)疫情進(jìn)行現(xiàn)場(chǎng)調(diào)查,采集患者咽拭子標(biāo)本,使用實(shí)時(shí)熒光定量PCR方法對(duì)標(biāo)本進(jìn)行流感樣病例篩查,篩查后的陰性標(biāo)本進(jìn)行呼吸道病毒核酸檢測(cè),用腺病毒六鄰體蛋白基因作為靶基因?qū)ο俨《境鹾Y陽(yáng)性標(biāo)本進(jìn)行擴(kuò)增并測(cè)序,測(cè)序結(jié)果在GenBank上進(jìn)行序列比較后使用BLAST和MEGA 5.0軟件進(jìn)行分析。該起疫情首例病例發(fā)病時(shí)間為2016年12月2日,末例病例發(fā)病時(shí)間為2016年12月17日,共發(fā)病34例,期間出現(xiàn)3次發(fā)病高峰,患者均為學(xué)生,其中男女生各17例,男女比例1∶1�；颊唧w溫均39℃,大部分有頭暈、頭痛癥狀,少數(shù)伴有咽痛、咳嗽癥狀。發(fā)病的四個(gè)班級(jí)間的罹患率差異有統(tǒng)計(jì)學(xué)意義。共采集8份咽拭子標(biāo)本,經(jīng)實(shí)時(shí)熒光定量PCR檢測(cè)均為腺病毒陽(yáng)性,其中1份合并鼻病毒陽(yáng)性。8份標(biāo)本用分型引物檢測(cè)并分析序列確定均為腺病毒E亞屬4型。此次急性呼吸道感染暴發(fā)疫情為連鎖式傳播,由腺病毒4型引起,毒株序列與深圳、國(guó)內(nèi)其他城市分離的腺病毒4型毒株序列具有高度同源性,提示該毒株可用于疫苗株篩選。早期發(fā)現(xiàn)可疑病例并采取隔離措施是預(yù)防腺病毒感染暴發(fā)的最佳手段。
[Abstract]:To explore the epidemiological and etiological characteristics of an outbreak of acute respiratory tract virus infection (ARV) in a primary school in Guangming New District of Shenzhen City in December 2016. The corresponding epidemiological case questionnaire was used to investigate the epidemic situation in the field, and the throat swabs of the patients were collected. A real-time fluorescence quantitative PCR method was used to screen influenza like cases and negative samples were screened for respiratory virus nucleic acids. The adenovirus Hexon protein gene was used as the target gene to amplify and sequence the primary screening positive specimens of adenovirus. Sequencing results were analyzed by BLAST and MEGA 5.0 software after sequence comparison on GenBank. The onset time of the first case was December 2, 2016, and the last case was December 17, 2016. There were three peaks in the onset of the disease, and the patients were all students. Among them, 17 cases were male and female students, the ratio of male to female was 1: 1.The temperature of the patients was 39 鈩，

本文編號(hào)：1672500