本文選題：呼吸道感染　切入點(diǎn)：呼吸道合胞病毒　出處：《山東醫(yī)藥》2017年03期 　論文類(lèi)型：期刊論文

【摘要】：目的觀察2014年度和2015年度武漢地區(qū)急性呼吸道感染(ARTIs)患兒鼻咽分泌物中呼吸道合胞病毒(RSV)、人偏肺病毒(h MPV)基因檢出情況,并分析RSV、h MPV感染的分布特點(diǎn)。方法 2014年1月~2015年12月收治的0~14歲的ARTIs患兒816例(2014年度456例、2015年度360例),采集患兒鼻咽分泌物,采用RTPCR檢測(cè)RSV G基因、h MPV F基因,通過(guò)DNA測(cè)序分析基因型。觀察RSV、h MPV感染在不同年度、季節(jié)及不同性別、年齡、病種患兒中的分布情況。結(jié)果 816例患兒中,檢出RSV 187例,其中2014年度109例、2015年度78例;檢出h MPV 116例,其中2014年度69例、2015年度47例;2014年度和2015年度h MPV、RSV的檢出率差異均無(wú)統(tǒng)計(jì)學(xué)意義。1~2歲年齡組患兒h MPV檢出率最高,5歲以內(nèi)患兒h MPV檢出率高于90%;5歲以內(nèi)患兒RSV檢出率也較高,高于90%。喘息性支氣管炎、毛細(xì)支氣管炎患兒h MPV檢出率顯著高于其他感染部位(P均0.05),上呼吸道感染、支氣管炎、支氣管肺炎患兒h MPV檢出率較低(10%左右);支氣管肺炎、毛細(xì)支氣管炎患兒RSV檢出率高于其他感染部位(P均0.05),喘息性支氣管炎患兒RSV檢出率最低。每年2~3月h MPV、RSV檢出率最高,6~9月最低。基因型分析顯示,2014、2015年度患兒檢出RSV基因型均以A型為主,2014年度患兒檢出h MPV基因型以A2型為主、2015年度以B型為主。結(jié)論 RSV和h MPV是武漢地區(qū)ARTIs患兒常見(jiàn)的病原體,RSV、h MPV感染多發(fā)生在5歲以下患兒,且多發(fā)于冬季和早春;RSV感染與支氣管肺炎、毛細(xì)支氣管炎有關(guān),h MPV感染與喘息性支氣管炎、毛細(xì)支氣管炎有關(guān);RSV流行毒株以A型為主,h MPV流行毒株在2014、2015年度存在差異。
[Abstract]:Objective to observe the detection of respiratory syncytial virus (RSVV) and human metapulmonary virus (hMPV) gene in nasopharyngeal secretions of children with ART Isa in Wuhan in 2014 and 2015. Methods from January 2014 to December 2015, 816 patients with ARTIs aged from 0 to 14 years old and 456 cases with ARTIs from 2014 to 2015 were collected and their nasopharyngeal secretions were collected. RTPCR was used to detect h MPV F gene of RSV G gene. Genotypes were analyzed by DNA sequencing. The distribution of RSVH MPV infection in different year, season, sex, age and disease was observed. Results 187 cases of RSV were detected in 816 cases, including 109 cases in 2014 and 78 cases in 2015. H MPV was detected in 116 cases. There was no significant difference in the detection rate of h MPV between the 47 cases in 2014 and 2015. The detection rate of h MPV in the 2 year old group was the highest. The detection rate of h MPV in children under 5 years old was higher than that in 90 years old children under 5 years old, and the detection rate of RSV was also higher in the age group of 2 years old than that in the age group of 90 years old. The detection rate of h MPV in children with asthmatic bronchitis and bronchiolitis was significantly higher than that in other infection sites (P 0.05). The detection rate of MPV in children with upper respiratory tract infection, bronchitis and bronchopneumonia was about 10%. The detection rate of RSV in children with bronchiolitis was higher than that in other infection sites (P < 0.05), and the detection rate of RSV in asthmatic bronchitis was the lowest. The detection rate of RSV was the highest in children with bronchiolitis from 2 h to March every year and the lowest from 6 to September. Genotype analysis showed that RSV was detected in children with bronchiolitis in 20142015. The h MPV genotypes were mainly detected by type A in 2014 and B in 2015. Conclusion RSV and h MPV are the common pathogens of ARTIs in Wuhan area, and most of them occur in children under 5 years of age. Most of RSV infection in winter and early spring were associated with bronchopneumonia, bronchiolitis related MPV infection and wheezing bronchitis, and bronchiolitis related serotype A was the main epidemic strain of MPV.
【作者單位】： 湖北省武漢市普仁醫(yī)院;
【分類(lèi)號(hào)】：R725.6
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本文編號(hào)：1625356