本文關(guān)鍵詞： 過敏性紫癜 紫癜性腎炎 血小板活化因子乙酰水解酶 血管內(nèi)皮生長(zhǎng)因子 熱休克蛋白70　出處：《寧夏醫(yī)科大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的通過檢測(cè)血小板活化因子乙酰水解酶（PAF-AH）在過敏性紫癜（HSP）及紫癜性腎炎（HSPN）中的表達(dá)，探討PAF-AH與HSP腎臟損害的關(guān)聯(lián)性，了解HSP患兒急性期血小板活化、血管內(nèi)皮損傷及凝血狀態(tài)的相關(guān)性，及其與腎臟損害的關(guān)系，，為研究HSP的發(fā)病機(jī)制和臨床治療提供一定的理論依據(jù)。 方法收集2010年10月-2011年4月在寧夏醫(yī)科大學(xué)總醫(yī)院兒科住院的初發(fā)過敏性紫癜患兒55例，并按有無腎臟損害將其分為非紫癜性腎炎（非HSPN）組30例和紫癜性腎炎（HSPN）組25例。另選體檢健康的40例兒童作為健康對(duì)照組。采用實(shí)時(shí)熒光定量PCR技術(shù)檢測(cè)PAF-AH的mRNA表達(dá)，同時(shí)采用雙抗體夾心酶聯(lián)免疫吸附法（ELISA）檢測(cè)血清PAF-AH、血管內(nèi)皮生長(zhǎng)因子（VEGF）、熱休克蛋白70（HSP70）蛋白水平變化。將PAF-AH與VEGF、HSP70水平進(jìn)行相關(guān)性分析。同時(shí)檢測(cè)血常規(guī)及凝血全套。檢測(cè)數(shù)據(jù)采用SPSS13.0軟件進(jìn)行處理。 結(jié)果1.紫癜性腎炎組PAF-AH mRNA及蛋白表達(dá)較非紫癜性腎炎及健康對(duì)照組患兒增高，差異有統(tǒng)計(jì)學(xué)意義（P0.01）；非紫癜性腎炎組PAF-AH蛋白表達(dá)較健康對(duì)照組患兒增高，差異有統(tǒng)計(jì)學(xué)意義（P0.01）；2.紫癜性腎炎組及非紫癜性腎炎組患兒血清VEGF、HSP70表達(dá)均較健康對(duì)照組增高，差異有統(tǒng)計(jì)學(xué)意義（P0.01）；紫癜性腎炎組VEGF、HSP70表達(dá)又高于非紫癜性腎炎組，差異有統(tǒng)計(jì)學(xué)意義（P0.01）；3.紫癜性腎炎組患兒PAF-AH與VEGF及PAF-AH與HSP70表達(dá)均呈正相關(guān)（P0.01）。4.紫癜性腎炎組及非紫癜性腎炎組PLT、MPV、PDW、PTA、PTR、INR、APTT-R差異有統(tǒng)計(jì)學(xué)意義，其中除MPV、PDW和PTA在紫癜性腎炎組增高外，其他指標(biāo)紫癜性腎炎組較非紫癜性腎炎組均下降。 結(jié)論1.PAF-AH參與了HSP的免疫發(fā)病機(jī)制，并與腎臟損害呈正相關(guān)。2.血小板活化、血管內(nèi)皮損傷及凝血狀態(tài)與急性期HSP腎臟損害的發(fā)生發(fā)展密切相關(guān)
[Abstract]:Objective to investigate the relationship between PAF-AH and renal damage of HSP by detecting the expression of PAF-AH in Henoch-Schonlein purpura (HSP) and Henoch-Schonlein purpura nephritis (Henoch-Schonlein purpura nephritis). The relationship between vascular endothelial injury and coagulation status, and the relationship between vascular endothelial injury and renal damage, provides a theoretical basis for the study of the pathogenesis and clinical treatment of HSP. Methods 55 cases of primary Henoch-Schonlein purpura were collected from October 2010 to April 2011 in the pediatrics department of Ningxia Medical University General Hospital. The patients were divided into two groups according to whether they had renal damage or not: 30 cases of non-Henoch-Schonlein purpura nephritis group and 25 cases of Henoch-Schonlein purpura nephritis group. 40 healthy children were selected as healthy control group. The mRNA expression of PAF-AH was detected by real-time fluorescence quantitative PCR. The levels of serum PAF-AH, vascular endothelial growth factor (VEGF) and heat shock protein 70 (HSP70) were detected by double antibody sandwich enzyme linked immunosorbent assay (Elisa). The correlation between PAF-AH and VEGF HSP70 was analyzed. The data are processed by SPSS13.0 software. Results 1. The expression of PAF-AH mRNA and protein in Henoch-Schonlein purpura nephritis group was significantly higher than that in non-purpura nephritis group and healthy control group (P 0.01), and the expression of PAF-AH protein in non-purpura nephritis group was higher than that in healthy control group. The expression of VEGF HSP70 in children with Henoch-Schonlein purpura nephritis and non-Henoch-Schonlein purpura nephritis was significantly higher than that in healthy controls (P 0.01), and the expression of VEGF HSP70 in Henoch-Schonlein purpura nephritis was higher than that in non-Henoch-Schonlein purpura nephritis. The expression of PAF-AH and VEGF, PAF-AH and HSP70 in Henoch-Schonlein purpura nephritis (HSPN) group were all positively correlated with that in Henoch-Schonlein purpura nephritis group and non-purpura nephritis group. There was a significant difference in the expression of PAF-AH and VEGF and PAF-AH and HSP70 in patients with Henoch-Schonlein purpura nephritis and non-purpura glomerulonephritis, and there was a significant difference in the expression of PAF-AH and PTA in Henoch-Schonlein purpura glomerulonephritis. Other indexes of Henoch-Schonlein purpura nephritis group were lower than that of non-purpura nephritis group. PAF-AH is involved in the immune pathogenesis of HSP, and is positively correlated with renal damage. 2.Platelet activation, vascular endothelial injury and coagulation are closely related to the development of renal damage in acute HSP.
【學(xué)位授予單位】：寧夏醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R725.5

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