本文關(guān)鍵詞： 基質(zhì)細(xì)胞衍生因子-1 轉(zhuǎn)化生長(zhǎng)因子-β1 腎小管間質(zhì)纖維化 單側(cè)輸尿管梗阻　出處：《山西醫(yī)科大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：探討幼鼠腎間質(zhì)纖維化模型(單側(cè)輸尿管結(jié)扎模型)中基質(zhì)細(xì)胞衍生因子-1(SDF-1)不同時(shí)間點(diǎn)的表達(dá)趨勢(shì)、相關(guān)性,以及用阿托伐他汀干預(yù)的效應(yīng)。 方法：建立幼年大鼠腎間質(zhì)纖維化模型。將90只幼鼠隨機(jī)分為對(duì)照組、藥物組和模型組,每組各30只。各組分別于實(shí)驗(yàn)第1d、3d、5d、7d、14d各時(shí)間點(diǎn)取腎組織進(jìn)行相關(guān)指標(biāo)測(cè)定。采用Masson的染色方法評(píng)價(jià)各組個(gè)時(shí)間點(diǎn)腎小管受損的程度。免疫組化法檢測(cè)大鼠腎組織中SDF-1和TGF-β1的蛋白表達(dá)趨勢(shì)及相關(guān)性。用SPSS13.0統(tǒng)計(jì)軟件進(jìn)行統(tǒng)計(jì)學(xué)處理。 結(jié)果：鏡下觀察UUO幼鼠腎間質(zhì)纖維化過(guò)程中SDF-1表達(dá)于腎小管上皮細(xì)胞,SDF-1在正常幼鼠腎組織中表達(dá)低微,而在腎纖維化過(guò)程中其表達(dá)水平明顯升高,而且隨著幼鼠腎纖維化程度的加重相應(yīng)地表達(dá)逐漸增高。對(duì)照組,模型組和用藥組的SDF-1灰度值相比較,顯示三組之間存在差別(F=9.04,P=0.001),用藥組灰度值高于模型組,其纖維化程度低于模型組；三組各個(gè)時(shí)間點(diǎn)的比較結(jié)果顯示,模型組(F=70.86,P0.001)和用藥組(F=92.28,P0.001)不同時(shí)間點(diǎn)的灰度值之間存在差別,模型組與用藥組在各時(shí)間點(diǎn)間的灰度值比較結(jié)果除了第一天差別無(wú)統(tǒng)計(jì)學(xué)意義之外(P0.05),其余各時(shí)間點(diǎn)之間均存在差別(P0.05)。TGF-β1的結(jié)果與SDF-1類似。實(shí)驗(yàn)顯示隨著腎纖維化程度的越重,TGF-β1表達(dá)趨勢(shì)越高,SDF-1蛋白表達(dá)趨勢(shì)越多,二者之間可能存在著相互作用。SDF-1蛋白表達(dá)趨勢(shì)與腎纖維化病變程度呈正相關(guān),而與各時(shí)間點(diǎn)各組的灰度值呈負(fù)相關(guān),即SDF-1蛋白表達(dá)趨勢(shì)越高,纖維化程度越重,其灰度值越低。 結(jié)論：SDF-1與腎間質(zhì)纖維化有著密切的關(guān)系,SDF-1的表達(dá)上調(diào),可能通過(guò)干預(yù)促纖維化因子TGF-β1的變化抑制了腎間質(zhì)纖維化的進(jìn)展。阿托伐他汀保護(hù)腎臟可能是通過(guò)下調(diào)SDF-1的表達(dá)實(shí)現(xiàn)的。
[Abstract]:Objective: to investigate the expression trend and correlation of stromal cell derived factor-1 SDF-1 in renal interstitial fibrosis model of young rats (unilateral ureteral ligation model) and the effect of Atto vastatin intervention. Methods: the renal interstitial fibrosis model of juvenile rats was established. 90 young rats were randomly divided into control group, drug group and model group. There were 30 rats in each group. The renal tissues were taken from each group on the 1st day, 3d, 5d, 7d and 14d, respectively. The degree of renal tubule damage was evaluated by Masson staining method. The renal tissue of rats was detected by immunohistochemical method. The expression trend and correlation of SDF-1 and TGF- 尾 1 were analyzed by SPSS13.0 software. Results: the expression of SDF-1 in renal tubuloepithelial cells of UUO young rats was observed to be low in normal renal tissues, but significantly increased in the process of renal fibrosis in the process of renal interstitial fibrosis in young UUO rats, and the expression of SDF-1 in renal tubuloepithelial cells was significantly increased in the process of renal fibrosis. The SDF-1 grayscale values of control group, model group and medication group were compared with each other, which showed that there was a difference between the three groups, and the gray value of drug group was higher than that of model group, and that of the control group was higher than that of the control group, and that of the model group was higher than that of the control group, and that of the model group was higher than that of the control group. The degree of fibrosis in the model group was lower than that in the model group, and the results of comparison between the three groups at different time points showed that there were differences in gray values between the model group and the medication group at different time points. The results of gray value comparison between the model group and the medication group at each time point were similar to that of SDF-1, except that there was no significant difference on the first day (P 0.05). The results of the other time points were similar to that of SDF-1. The higher the degree of TGF- 尾 1 expression, the higher the expression trend of SDF-1 protein. There may be a positive correlation between the expression trend of SDF-1 protein and the degree of renal fibrosis, but a negative correlation with the gray value of each group at each time point, that is, the higher the expression trend of SDF-1 protein is, the more serious the fibrosis degree is, and the lower the gray value is. Conclusion there is a close relationship between the expression of SDF-1 and renal interstitial fibrosis. The change of TGF- 尾 1 may inhibit the progress of renal interstitial fibrosis. Atto vastatin may protect the kidney by down-regulating the expression of SDF-1.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R726.9

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