本文關(guān)鍵詞： 手足口病 EV IgM抗體 熒光定量RT-PCR　出處：《現(xiàn)代預(yù)防醫(yī)學(xué)》2017年10期 　論文類(lèi)型：期刊論文

【摘要】：目的探討檢測(cè)血清EV71-IgM抗體在EV71感染手足口病早期診斷中的應(yīng)用價(jià)值。方法綜合收集廣西地區(qū)2014年4月-10月和2016年4月-10月臨床診斷手足口病患兒436例,對(duì)所有患兒血清進(jìn)行EV71-IgM抗體檢測(cè)。其中136例患兒同時(shí)采集咽拭子或肛拭子通過(guò)熒光定量RT-PCR檢測(cè)EV71-RNA。錄入數(shù)據(jù)后采用SPSS 17.0進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果臨床診斷手足口病436人中EV71-IgM抗體陽(yáng)性256例,陰性180例,危重癥組和重癥組的EV71-IgM抗體陽(yáng)性率高于輕癥組,差異有統(tǒng)計(jì)學(xué)意義(χ2=88.016,P0.001)。以熒光定量RT-PCR檢測(cè)為標(biāo)準(zhǔn),同時(shí)檢測(cè)136例患兒肛拭子或咽拭子EV71-RNA和血清EV71-IgM抗體,2種方法的檢測(cè)一致性中等可靠(Kappa=0.575,P0.001)。EV71-IgM檢測(cè)的靈敏度為93.67%(74/79),特異度為61.40%(35/57)。EV71-IgM抗體陽(yáng)性檢出率在病程1~3 d逐漸升高,在病程d3檢出率達(dá)到92.31%,在病程4~10 d檢出率達(dá)到100.00%。結(jié)論血清EV71-IgM抗體檢測(cè)具有較高的靈敏度,可行性強(qiáng),操作方便,對(duì)早期診斷手足口病病原學(xué)有良好的應(yīng)用價(jià)值。
[Abstract]:Objective to investigate the value of detecting serum EV71-IgM antibody in the early diagnosis of HFMD infected with EV71. Methods Clinical diagnosis of HFMD in Guangxi from April 2014 to October and April 2016 to October were collected. EV71-IgM antibody was detected in serum of all children, among them, throat swabs or anal swabs were collected simultaneously by fluorescence quantitative RT-PCR to detect EV71-RNA.After entering the data, SPSS 17.0 was used for statistical analysis. Results Clinical diagnosis of HFMD 436. There were 256 positive cases of EV71-IgM antibody in human. The positive rate of EV71-IgM antibody in critically ill group and severe group was significantly higher than that in mild group (蠂 2 88.016 P 0.001). Fluorescence quantitative RT-PCR was used as the standard. At the same time, the two methods of detecting anal swab or pharyngeal swab EV71-RNA and serum EV71-IgM antibody were consistent and reliable. The sensitivity was 93.6774 / 79 and the specificity was 61.400.35 / 57.EV71-IgM antibody positive rate increased gradually at 1 day after the disease course. The detectable rate of serum EV71-IgM antibody was 92.31 on the day of the disease course and 10 days after the course of the disease. Conclusion the detection of serum EV71-IgM antibody is sensitive, feasible and easy to operate. It has a good application value in the early diagnosis of hand, foot and mouth disease etiology.
【作者單位】： 廣西醫(yī)科大學(xué)第一附屬醫(yī)院兒科;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(81460251) 廣西壯族自治區(qū)手足口病防治研究資金(2014249)
【分類(lèi)號(hào)】：R725.1

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