本文關(guān)鍵詞：2940nm Er：YAG聯(lián)合兒茶素治療鼠皮膚光老化的實(shí)驗(yàn)研究　出處：《南昌大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

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【摘要】：目的： 探究2940nm Er：YAG激光、兒茶素抗鼠皮膚光老化作用，進(jìn)一步探討兩者對于光老化可能的作用機(jī)理。 方法： 根據(jù)不同的處理方法，將60只昆明小鼠為5組：A（對照組）、B（光老化組）、C（光老化+激光組）、D （光老化+兒茶素組）、E（光老化+激光+兒茶素組），每組各12只，對干預(yù)（除對照組）小鼠行持續(xù)8周紫外線照射、持續(xù)4周兒茶素、激光處理后，應(yīng)用HE、免疫組化、Western blot及生物檢測等方法對各組小鼠皮膚結(jié)構(gòu)、超氧化物岐化酶SOD活力、丙二醛MDA含量、Ⅰ型膠原平均光密度AOD及MMP-1蛋白表達(dá)量進(jìn)行檢測。 結(jié)果： B（光老化組）小鼠皮膚肉眼觀干燥、粗糙、皮溝明顯、色素沉著明顯；HE染色顯示小鼠皮膚表皮層增厚厚度不均一，角質(zhì)層增生偶可見脫落；真皮層膠原減少，厚度變薄，纖維層斷裂，膠原纖維變粗條索化，腺體增生；與其余各組小鼠相比SOD活力明顯降低（P㩳0.01），MDA含量明顯增多（P㩳0.01）,Ⅰ型膠原免疫組化見真皮層新生膠原減少，真皮層顯著變薄，平均光密度AOD明顯降低（P㩳0.01）；MMP-1蛋白表達(dá)明顯增高（P㩳0.01）。 與光老化組相比，各干預(yù)組小鼠皮膚均有明顯改善，皮膚較光滑、紅潤、光澤度好、細(xì)膩、動態(tài)性皺紋基本消失、無明顯色素沉著；HE染色可見表皮層結(jié)構(gòu)完整，但仍可見角質(zhì)層脫落，真皮層變厚，可見新生膠原，腺體稍增多，排列較均勻；SOD活力明顯升高（＊P㩳0.01；◆P㩳0.05）；MDA含量明顯下降，均具有顯著性差異（＊P㩳0.01）,Ⅰ型膠原免疫組化見真皮層新生膠原增多，真皮層顯著增厚，平均光密度AOD明顯升高（＊P㩳0.01）；MMP-1蛋白表達(dá)明顯減少（＊P㩳0.01）。 E與A、D組相較MMP-1表達(dá)量具有統(tǒng)計(jì)學(xué)意義（◆P㩳0.05；＊P㩳0.01），E與C組相較SOD活力值、MDA含量均有顯著性差異（＊P㩳0.01）。 結(jié)論： 2940nm Er：YAG激光、兒茶素均可促進(jìn)皮膚緊致年輕化、刺激膠原再生、增強(qiáng)SOD活性、降低MDA含量，減少M(fèi)MP-1蛋白表達(dá)，兩者聯(lián)合相較于單獨(dú)使用激光、兒茶素療效更佳，，可能為效果的疊加，這值得進(jìn)一步深入研究，為臨床治療和研究提供可靠依據(jù)。
[Abstract]:Objective: The effects of catechin on photoaging of mouse skin were investigated by 2940nm Er:YAG laser, and the possible mechanism of photoaging was discussed. Methods: According to different treatment methods, 60 Kunming mice were divided into 5 groups (control group): control group (control group) (photoaging group) (photoaging laser group) (photoaging group). E- (photoaging laser catechin group, 12 rats in each group) were treated by ultraviolet irradiation for 8 weeks and catechin for 4 weeks. After laser treatment, HEH and immunohistochemistry were used. The skin structure, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content of mice were determined by Western blot and bioassay. The average optical density of type I collagen AOD and MMP-1 protein expression were detected. Results: B (light aging group) mice skin dry, rough, skin sulcus obvious, pigmentation; He staining showed that the thickness of the skin epidermis was not uniform, and the exfoliation of the cuticle was occasionally seen. The cortical collagen decreased, the thickness became thinner, the fibrous layer was broken, the collagen fibers became thicker and striped, and the glands proliferated. Compared with the other groups, the activity of SOD decreased significantly. The content of MDA increased significantly (P < 0.05). The immunohistochemical staining of type I collagen showed that the new collagen in the dermis decreased, the cortex thinned significantly, and the mean optical density (AOD) decreased significantly. The expression of MMP-1 protein increased significantly. 0.01g. Compared with the light aging group, the skin of each intervention group was obviously improved, the skin was smooth, ruddy, glossy, delicate, dynamic wrinkles disappeared basically, no obvious pigmentation. The structure of the epidermis was intact in HE staining, but the cuticular layer was shedding, the dermis became thicker, the new collagen was visible, the glands increased slightly, and the arrangement was even. The activity of SOD increased significantly. 0.01; P? The content of MDA decreased obviously, and there was significant difference in the content of MDA between 0. 05% and 0. 05% (P < 0. 05). The immunohistochemical staining of type I collagen showed the increase of new collagen in the dermis, the thickening of the dermis, and the increase of the average optical density (AOD). The expression of MMP-1 protein decreased significantly. 0.01g. The expression of MMP-1 in E group was significantly higher than that in Agna D group (P? 0.05? There was significant difference in SOD activity between group E and group C (P < 0.01). 0.01g. Conclusion: At 2940nm Er:YAG laser, catechins could promote skin aging, stimulate collagen regeneration, enhance SOD activity, decrease MDA content and decrease MMP-1 protein expression. Compared with laser alone, catechin has better curative effect and may be the superposition of effect, which is worthy of further study and provides reliable basis for clinical treatment and research.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R62

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