【摘要】：豬偽狂犬病(Pseudorabies,PR)是由偽狂犬病毒(Pseudorabiesvirus,PRV)引起的一種急性傳染病,給畜牧業(yè),特別是養(yǎng)豬業(yè)發(fā)展造成巨大經(jīng)濟(jì)損失。為了制定符合當(dāng)?shù)厣a(chǎn)特點(diǎn)的PR凈化方案,本課題于2012至2016年期間采用gE-ELISA方法對延平區(qū)養(yǎng)殖場豬群PRV野毒感染的情況進(jìn)行血清學(xué)調(diào)查,并通過制定PR綜合防控措施,對延平區(qū)某規(guī)�；B(yǎng)豬場開展PR凈化試點(diǎn)工作,為該區(qū)域PR防制工作提供參考,結(jié)果如下:1.在2012年1月至2016年12月期間,我們采集了福建省南平市延平區(qū)各鄉(xiāng)鎮(zhèn)不同規(guī)模豬場不同批次共2945份血清樣品。流行病學(xué)調(diào)查結(jié)果表明:(1)延平區(qū)規(guī)�；i場的豬群豬偽狂犬病毒gE陽性率在2014年之前呈逐年遞增趨勢,其中2013到2014年之間出現(xiàn)快速上升達(dá)到31.9%,之后開始下降;(2)不同地區(qū)的豬群豬偽狂犬病毒gE陽性率為6.5%-44.8%,其中規(guī)�；B(yǎng)殖程度越高的地區(qū)豬群豬偽狂犬病毒gE陽性率越低;(3)不同規(guī)模的豬群豬偽狂犬病毒gE陽性率為21.4%-37.6%,其中養(yǎng)豬場的規(guī)模越大其豬偽狂犬病毒gE陽性性率越低;(4)不同類型豬群的豬群豬偽狂犬病毒gE陽性率為19.7%-37.9%,其中后備母豬的豬偽狂犬病毒gE陽性率最低,經(jīng)產(chǎn)母豬的豬偽狂犬病毒gE陽性率最高。2.基于此次豬偽狂犬病毒gE陽性率調(diào)查結(jié)果,我們制定了 PR綜合防控措施并在本區(qū)域內(nèi)三個不同PR污染程度的規(guī)�；i場中試點(diǎn)。綜合防控措施包括:嚴(yán)格的種源管理、嚴(yán)格的豬群PRV疫苗免疫程序、定期的豬群PRV抗體及野毒感染監(jiān)測、常規(guī)的無害化處理以及生產(chǎn)管理制度。經(jīng)過三年(2014-2016年期)PR綜合防控措施的實(shí)施,PR重度污染豬場的PRV-gE抗體陽性率從91.5%下降到24.7%,PR中度污染豬場的PRV-gE抗體陽性率從39.0%下降到3.1%,PR輕度污染豬場的PRV-gE抗體陽性率從8.6%下降到0.2%,而同期當(dāng)?shù)胤荘R凈化試點(diǎn)豬場的PRV-gE抗體陽性率則從20.2%上升到30.7%。綜上所述,通過流行病學(xué)調(diào)查我們發(fā)現(xiàn)延平區(qū)規(guī)�；i場的PRV感染呈現(xiàn)出與養(yǎng)殖方式以及養(yǎng)殖規(guī)模密切相關(guān)的特點(diǎn)。為實(shí)現(xiàn)PR防制目標(biāo),我們認(rèn)為養(yǎng)豬場在采取PR凈化一般措施的基礎(chǔ)上,不同豬場還應(yīng)制定和實(shí)施針對性PR凈化措施。
[Abstract]:Pseudorabies virus (PR) is an acute infectious disease caused by pseudorabies virus (PRV), which has caused great economic losses to animal husbandry, especially to the development of pig industry. In order to establish PR purification scheme according to the local production characteristics, the gE-ELISA method was used to investigate serological infection of PRV wild virus in swine farms in Yanping district from 2012 to 2016, and PR comprehensive prevention and control measures were established. A pilot project of PR purification was carried out in a large-scale pig farm in Yanping District, which provided a reference for PR prevention and control in this area. The results are as follows: 1. From January 2012 to December 2016, we collected 2945 serum samples from pig farms of different sizes in different villages and towns in Yanping District, Nanping City, Fujian Province. The results of epidemiological investigation showed that: (1) the positive rate of pseudorabies virus (GE) in large scale pig farms in Yanping District was increasing year by year before 2014. The positive rate of pseudorabies virus (GE) in different areas was 6.5-44.8, and the positive rate of pseudorabies virus (GE) was higher in the areas where the scale of breeding was higher. (3) the positive rate of pseudorabies virus (GE) was 21.4-37.6g in pig herd of different size, in which, the larger the scale of pig farm, the lower the positive rate of pseudorabies virus (GE); (4) the positive rate of pseudorabies virus (GE) was 19.7-37.9in different types of pig herd. The positive rate of boar pseudorabies virus (GE) was the lowest in sows. The positive rate of pseudorabies virus (GE) was the highest in sows. Based on the results of the investigation on the positive rate of porcine pseudorabies virus (PRV), we have established comprehensive prevention and control measures for PR and tested them in three large-scale pig farms with different PR pollution levels in our region. Comprehensive prevention and control measures include: strict provenance management, strict immunization procedures for swine PRV vaccine, regular surveillance of swine PRV antibody and wild virus infection, routine innocuous treatment and production management system. After three years (2014-2016) implementation of comprehensive PR prevention and control measures, the positive rate of PRV-gE antibody in PR heavily polluted pig farm decreased from 91.5% to 24.7R moderately polluted pig farm from 39.0% to 3.1%. The positive rate of PRV-gE antibody in mildly polluted pig farm decreased from 91.5% to 24.7%. The positive rate of PRV-gE antibody increased from 20.2% to 30.7% in the local non-PR experimental pig farm during the same period. To sum up, we found that the PRV infection in large-scale pig farms in Yanping district was closely related to the way of breeding and the scale of breeding. In order to achieve the goal of PR prevention and control, we think that different pig farms should also formulate and implement targeted PR purification measures on the basis of the general measures of PR purification.
【學(xué)位授予單位】：福建農(nóng)林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S858.28

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