本文選題：豬傳染性胃腸炎 + 豬流行性腹瀉　； 參考：《江西農(nóng)業(yè)大學(xué)》2017年碩士論文

【摘要】：豬傳染性胃腸炎(Swine Transmissible Gastroenteritis,TGE)和豬流行性腹瀉(Porcine Epidemic Diarrhea,PED)均是由冠狀病毒引起,可造成患病仔豬出現(xiàn)嘔吐、腹瀉及死亡等臨床癥狀,是危害豬群健康的兩種重要疫病。由于市場(chǎng)供應(yīng)的疫苗質(zhì)量參差不齊,大部分疫苗的中和抗體水平較低,對(duì)豬群保護(hù)的效果不理想,給養(yǎng)豬業(yè)帶來了巨大的經(jīng)濟(jì)損失。我們通過開展對(duì)兩種病毒敏感細(xì)胞的篩選、病毒的濃縮、免疫佐劑的篩選、免疫增強(qiáng)劑的添加、病毒液保存條件及病毒含量變化實(shí)驗(yàn)、腸毒的制備并滅活等實(shí)驗(yàn),對(duì)豬傳染性胃腸炎、豬流行性腹瀉二聯(lián)滅活疫苗的生產(chǎn)工藝進(jìn)行了優(yōu)化并制備了樣品,隨后,免疫3日齡陰性仔豬后檢測(cè)其免后兩周中和抗體水平。根據(jù)本次實(shí)驗(yàn)結(jié)果,我們總結(jié)如下:(1)篩選出較敏感細(xì)胞PK-15-1和VERO-1兩株,豬傳染性胃腸炎和豬流行性腹瀉效價(jià)TCID50均在10-7/ml以上,且豬傳染性胃腸炎合格率90%以上,豬流行性腹瀉合格率100%;(2)PEDV制備后一周之內(nèi)滅活并配苗,TGEV兩周之內(nèi)滅活并配苗能夠保證成品質(zhì)量良好;(3)提高兩種病毒的病毒含量,采取對(duì)疫苗進(jìn)行沉淀后除去五分之二上清液的工藝可以提高豬傳染性胃腸炎和豬流行性腹瀉二聯(lián)滅活疫苗的中和抗體;(4)相同濃度下,選擇顆粒越細(xì),表面積越大的氫氧化鋁膠佐劑可以提高豬傳染性胃腸炎和豬流行性腹瀉兩種抗原中和抗體水平。以上實(shí)驗(yàn)在一定程度上可達(dá)到提高疫苗產(chǎn)品質(zhì)量和防疫效果的目的。對(duì)部分工藝進(jìn)行固化,從而實(shí)現(xiàn)優(yōu)化豬傳染性胃腸炎、豬流行性腹瀉二聯(lián)滅活疫苗工藝的目標(biāo)。本研究為優(yōu)化豬傳染性胃腸炎、豬流行性腹瀉二聯(lián)滅活疫苗的工藝提供了參考。
[Abstract]:Porcine Epidemic diarrhea and porcine Epidemic diarrhea caused by coronavirus can cause vomiting, diarrhea and death in sick piglets. They are two important infectious diseases which are harmful to the health of pigs. Due to the uneven quality of vaccines supplied in the market, the neutralizing antibody level of most vaccines is low, and the effect of protecting pigs is not ideal, which has brought huge economic losses to the pig industry. We conducted experiments on the screening of sensitive cells for the two viruses, the concentration of viruses, the screening of immune adjuvants, the addition of immune enhancers, the preservation conditions of virus fluids and the changes in virus contents, the preparation and inactivation of enterotoxins, etc. The production process of the inactivated vaccine against infectious gastroenteritis and epidemic diarrhea in pigs was optimized and the samples were prepared. Then the antibody levels were detected after immunizing 3-day-old negative piglets. According to the results of this experiment, we summarized as follows: 1) two sensitive cell strains, PK-15-1 and VERO-1, were screened out. The titers of TCID50 of porcine infectious gastroenteritis and porcine epidemic diarrhea were above 10-7/ml, and the qualified rate of infectious gastroenteritis was over 90%. The qualified rate of porcine epidemic diarrhea was 100%. The virus content of the two viruses was increased by inactivation and inoculation of TGEV within two weeks after the preparation of PEDV. When the supernatant of 2/5 was removed by precipitating the vaccine, the neutralizing antibody of the inactivated vaccine of infectious gastroenteritis and epidemic diarrhea in pigs could be increased at the same concentration, the finer the selected particles were, The larger the surface area of aluminum hydroxide gel adjuvant, the higher the neutralizing and antibody levels of infectious gastroenteritis and epidemic diarrhea. To a certain extent, the above experiments can improve the quality of vaccine products and the effect of epidemic prevention. In order to achieve the goal of optimizing the inactivated vaccine process of infectious gastroenteritis and epidemic diarrhea in pigs, some of the processes were solidified. This study provides a reference for optimizing the inactivated vaccine of infectious gastroenteritis and epidemic diarrhea in pigs.
【學(xué)位授予單位】：江西農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：S858.28

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