紡織工業(yè)是染料的主要用戶,它每年要排放大量的染料廢水,嚴重威脅著環(huán)境的安全。染料廢水的治理勢在必行。微生物修復已被證明是治理紡織染料污染最理想、最經濟的方法。一些微生物能對染料進行脫色和降解,可用于修復紡織染料污染的環(huán)境。其中,產電菌受到更多的關注,因為它對環(huán)境污染物具有非特異性還原能力,即它通過新陳代謝過程中釋放的電子來降解污染物。而且這種降解能力還可以通過人工電子遞質予以強化,這一點對難生物降解染料(如金屬復合偶氮染料)特別有用。因此,本研究以中性紅(NR)作為電子遞質,利用模式產電細菌Shewanella oneidensis MR-1,探究了難降解金屬復合偶氮染料酸性蘭193(AB 193)的生物可降解性。首先對AB 193的直接生物降解和電子遞質強化下的生物降解情況進行研究,建立起NR強化下的AB 193厭氧生物脫色體系,并對脫色體系的影響因素進行研究,然后用響應面法(RSM)優(yōu)化該脫色體系,并研究了各脫色參數之間的交互作用,最后對該體系的生物脫色機理和化學脫色機理進行進一步研究,并對脫色產物的植物毒性進行了評估。本研究的開展為難降解金屬復合偶氮染料廢水處理提供了新的思路,同...

【文章頁數】：91 頁

【學位級別】：碩士

【文章目錄】：
abstract
摘要
Chapter 1 Literature review
    1.1 Introduction
    1.2 Brief introduction to azo dyes
    1.3 Source of azo dye wastewater and its potential threat to the environment
    1.4 Available technologies for the treatment of azo dye containing textile wastewater
        1.4.1 Physical methods
        1.4.2 Chemical methods
        1.4.3 Biological methods
    1.5 Electrogenic bacteria
        1.5.1 Anaerobic respiration mechanism of S.oneidensis MR-1
        1.5.2 Extracellular electron transfer of S.oneidensis MR-1
        1.5.3 Electron mediator
        1.5.4 Recent applications of Shewanella in azo dye degradation and detoxification
    1.6 Goal and contents of this study
        1.6.1 The goal of the study
        1.6.2 Research contents
Chapter 2 Anaerobic decolorization of AB 193 by S.oneidensis MR-1 with NR as a redox mediator
    2.1 Introduction
    2.2 Material and methods
        2.2.1 Laboratory reagents and instrumentation
        2.2.2 Strains and culture conditions
        2.2.3 Biological decolorization experiment of AB 193
        2.2.4 Influencing factors of AB 193 reduction decolorization by S.oneidensis MR-1
        2.2.5 Toxicity evaluation of AB 193 and its decolorized products
    2.3 Results and discussion
        2.3.1 Influences of electron mediators on AB 193 decolorization by S.oneidensis MR-1
        2.3.2 Biodecolorization of AB 193 by S.oneidensis MR-1 using NR as electron mediator
        2.3.3 Effects of operation parameters on AB 193 decolorization by S.oneidensis MR-1 mediated with NR
    2.4 Phytotoxicity evaluation
    2.5 Conclusions
Chapter 3 Optimization of AB 193 decolorization using RSM
    3.1 Introduction
    3.2 Materials and method
        3.2.1 Experimental reagent and apparatus
        3.2.2 Strains and culture conditions
        3.2.3 Optimization of process parameters using RSM based on BBD
    3.3 Results and discussion
        3.3.1 Box-Behnken analysis
        3.3.2 Model adequacy
        3.3.3 Effects of interactive factors in AB 193 decolorization by S.oneidensis MR-1
        3.3.4 Model validation and confirmation
        3.3.5 Conclusions
Chapter 4 Anaerobic decolorization mechanism of AB 193 by S.oneidensis MR-1 with NR as an electron mediator
    4.1 Introduction
    4.2 Materials and methods
        4.2.1 Laboratory Reagents and Apparatus
        4.2.2 Strains and Culture conditions
        4.2.3 Neutral red reoxidation assay in AB 193 decolorization
        4.2.4 EPS extraction and composition analysis
        4.2.5 Experiments of Mtr mutants and inhibitors
        4.2.6 AB 193 decolorized product analysis
    4.3 Results and discussion
        4.3.1 Oxidation-reduction change of NR by S.oneidensis MR-1 and AB 193
        4.3.2 Effects of NR on EPS secretion of S.oneidensis MR-1 during AB 193 decolorization
        4.3.3 Effects of NR on Mtr pathway of S. oneidensis MR-1 during AB 193 reduction
        4.3.4 Effects of electron transport chain inhibitors on decolorization of AB 193
        4.3.5 Analysis of the decolorized product of AB 193
    4.4 Conclusion
Chapter 5 Conclusion and Recommendation
    5.1 Conclusions
    5.2 Recommendations
References
Acknowledgement
Appendix A Instrumentation
Appendix B Reagent



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