紡織工業(yè)是染料的主要用戶,它每年要排放大量的染料廢水,嚴(yán)重威脅著環(huán)境的安全。染料廢水的治理勢(shì)在必行。微生物修復(fù)已被證明是治理紡織染料污染最理想、最經(jīng)濟(jì)的方法。一些微生物能對(duì)染料進(jìn)行脫色和降解,可用于修復(fù)紡織染料污染的環(huán)境。其中,產(chǎn)電菌受到更多的關(guān)注,因?yàn)樗鼘?duì)環(huán)境污染物具有非特異性還原能力,即它通過(guò)新陳代謝過(guò)程中釋放的電子來(lái)降解污染物。而且這種降解能力還可以通過(guò)人工電子遞質(zhì)予以強(qiáng)化,這一點(diǎn)對(duì)難生物降解染料(如金屬?gòu)?fù)合偶氮染料)特別有用。因此,本研究以中性紅(NR)作為電子遞質(zhì),利用模式產(chǎn)電細(xì)菌Shewanella oneidensis MR-1,探究了難降解金屬?gòu)?fù)合偶氮染料酸性蘭193(AB 193)的生物可降解性。首先對(duì)AB 193的直接生物降解和電子遞質(zhì)強(qiáng)化下的生物降解情況進(jìn)行研究,建立起NR強(qiáng)化下的AB 193厭氧生物脫色體系,并對(duì)脫色體系的影響因素進(jìn)行研究,然后用響應(yīng)面法(RSM)優(yōu)化該脫色體系,并研究了各脫色參數(shù)之間的交互作用,最后對(duì)該體系的生物脫色機(jī)理和化學(xué)脫色機(jī)理進(jìn)行進(jìn)一步研究,并對(duì)脫色產(chǎn)物的植物毒性進(jìn)行了評(píng)估。本研究的開展為難降解金屬?gòu)?fù)合偶氮染料廢水處理提供了新的思路,同...

【文章頁(yè)數(shù)】：91 頁(yè)

【學(xué)位級(jí)別】：碩士

【文章目錄】：
abstract
摘要
Chapter 1 Literature review
    1.1 Introduction
    1.2 Brief introduction to azo dyes
    1.3 Source of azo dye wastewater and its potential threat to the environment
    1.4 Available technologies for the treatment of azo dye containing textile wastewater
        1.4.1 Physical methods
        1.4.2 Chemical methods
        1.4.3 Biological methods
    1.5 Electrogenic bacteria
        1.5.1 Anaerobic respiration mechanism of S.oneidensis MR-1
        1.5.2 Extracellular electron transfer of S.oneidensis MR-1
        1.5.3 Electron mediator
        1.5.4 Recent applications of Shewanella in azo dye degradation and detoxification
    1.6 Goal and contents of this study
        1.6.1 The goal of the study
        1.6.2 Research contents
Chapter 2 Anaerobic decolorization of AB 193 by S.oneidensis MR-1 with NR as a redox mediator
    2.1 Introduction
    2.2 Material and methods
        2.2.1 Laboratory reagents and instrumentation
        2.2.2 Strains and culture conditions
        2.2.3 Biological decolorization experiment of AB 193
        2.2.4 Influencing factors of AB 193 reduction decolorization by S.oneidensis MR-1
        2.2.5 Toxicity evaluation of AB 193 and its decolorized products
    2.3 Results and discussion
        2.3.1 Influences of electron mediators on AB 193 decolorization by S.oneidensis MR-1
        2.3.2 Biodecolorization of AB 193 by S.oneidensis MR-1 using NR as electron mediator
        2.3.3 Effects of operation parameters on AB 193 decolorization by S.oneidensis MR-1 mediated with NR
    2.4 Phytotoxicity evaluation
    2.5 Conclusions
Chapter 3 Optimization of AB 193 decolorization using RSM
    3.1 Introduction
    3.2 Materials and method
        3.2.1 Experimental reagent and apparatus
        3.2.2 Strains and culture conditions
        3.2.3 Optimization of process parameters using RSM based on BBD
    3.3 Results and discussion
        3.3.1 Box-Behnken analysis
        3.3.2 Model adequacy
        3.3.3 Effects of interactive factors in AB 193 decolorization by S.oneidensis MR-1
        3.3.4 Model validation and confirmation
        3.3.5 Conclusions
Chapter 4 Anaerobic decolorization mechanism of AB 193 by S.oneidensis MR-1 with NR as an electron mediator
    4.1 Introduction
    4.2 Materials and methods
        4.2.1 Laboratory Reagents and Apparatus
        4.2.2 Strains and Culture conditions
        4.2.3 Neutral red reoxidation assay in AB 193 decolorization
        4.2.4 EPS extraction and composition analysis
        4.2.5 Experiments of Mtr mutants and inhibitors
        4.2.6 AB 193 decolorized product analysis
    4.3 Results and discussion
        4.3.1 Oxidation-reduction change of NR by S.oneidensis MR-1 and AB 193
        4.3.2 Effects of NR on EPS secretion of S.oneidensis MR-1 during AB 193 decolorization
        4.3.3 Effects of NR on Mtr pathway of S. oneidensis MR-1 during AB 193 reduction
        4.3.4 Effects of electron transport chain inhibitors on decolorization of AB 193
        4.3.5 Analysis of the decolorized product of AB 193
    4.4 Conclusion
Chapter 5 Conclusion and Recommendation
    5.1 Conclusions
    5.2 Recommendations
References
Acknowledgement
Appendix A Instrumentation
Appendix B Reagent



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