本文關(guān)鍵詞：香港保健協(xié)會資助項(xiàng)目，由筆耕文化傳播整理發(fā)布。

淘豆網(wǎng)網(wǎng)友wo1230近日為您收集整理了關(guān)于“PDCD5、Bco2蛋白在宮頸鱗癌中的表達(dá)及藍(lán)萼甲素對宮頸鱗癌SiHa細(xì)胞的作用機(jī)制探討的文檔，，希望對您的工作和學(xué)習(xí)有所幫助。以下是文檔介紹：——————————1、’蘇州大學(xué)學(xué)位論文獨(dú)創(chuàng)性聲明本人鄭重聲明:所提交的學(xué)位論文是本人在導(dǎo)師的指導(dǎo)下,獨(dú)立進(jìn)行研究工作所取得的成果。除文中已經(jīng)注明引用的內(nèi)容外,本論文不含其他個(gè)人或集體已經(jīng)發(fā)表或撰寫過的研究成果,也不含為獲得蘇州大學(xué)或其它教育機(jī)構(gòu)的學(xué)位證書而使用過的材料。對本文的研究作出重要貢獻(xiàn)的個(gè)人和集體,均已在文中以明確方式標(biāo)明。本人承擔(dān)本聲明的法律責(zé)任。論文作者簽名:孺緲一日蘇州大學(xué)學(xué)位論文使用授權(quán)聲明本人完全了解蘇州大學(xué)關(guān)于收集、保存和使用學(xué)位論文的規(guī)定,即:學(xué)位論文著作權(quán)歸屬蘇州大學(xué)。本學(xué)位論文電子文檔的內(nèi)容和紙質(zhì)論文的內(nèi)容相一致。蘇州大學(xué)有權(quán)向國家圖書館、中國社科院文獻(xiàn)信息情報(bào)中心、中國科學(xué)技術(shù)信息研究所(含萬方數(shù)據(jù)電子出版社)、中國學(xué)術(shù)期刊(光盤版)電子雜志社送交本學(xué)位論文的復(fù)印件和電子文檔,允許論文被查閱和借閱,可以采用影印、縮印或其他復(fù)制手段保存和匯編學(xué)位論文,可以將學(xué)位論文的全部或部分內(nèi)容編入有關(guān)數(shù)據(jù)庫進(jìn)行檢索。涉密論文口本學(xué)位論文屬在——年一月解密后適用本規(guī)定。非涉密論文吵論文作者簽名:丕塹竺日期:2里叢絲呈導(dǎo)師簽名:j蜂日期:出本課題為香港保健協(xié)會資助項(xiàng)目(編號:20080908—4HK)論文指導(dǎo)小組王修珍副教授顧振綸教授蔣小崗副教授郭次儀教授PDCD5、Bcl-2蛋白在宮頸鱗癌中的表達(dá)及藍(lán)萼甲素對宮頸鱗癌SiHa細(xì)胞的作用機(jī)制探討中文摘要中文摘要目的:研究PDCD5、Bcl.2蛋白在宮頸鱗狀細(xì)胞癌(cervical squamous cellcarcinoma,)組織中的表達(dá)及與臨床病理的關(guān)系;觀察藍(lán)萼甲素(Glaucocalyxin A)對宮頸癌SiHa細(xì)胞的作用并探討其機(jī)制。方法:組織標(biāo)本89例及正常宮頸上皮(normal cervical epithelium,NCE)組織標(biāo)本25例,采用免疫組織化學(xué)法,組織及NCE組織中PDCD5、Bcl.2蛋白的表達(dá)情況;四***偶氮唑鹽(methyl thiazolyl tetrazolium,MTT)比色法觀察1.25pmol/L、2.51amol/L、5pmol/L、lOmnol/L、20pmol/L的Glaucocalyxin A對SiHa細(xì)胞增殖的影響;Hoechst33258熒光染色和透射電鏡(transmission electron microscopy,TEM)觀察給藥后SiHa細(xì)胞的形態(tài)學(xué)變化:流式細(xì)胞儀(flow cytometer,FCM)檢測給藥后SiHa細(xì)胞周期和凋亡率的變化;RT-PCR檢測給藥后SiHa細(xì)胞凋亡相關(guān)基因PDCD5、Caspase.3、Caspase.9、p53、Bcl-2、Bax mRNA表達(dá)的變化;Western blot檢測給藥后SiHa細(xì)胞凋亡相關(guān)蛋白PDCD5、Bcl.2、Bax蛋白的表達(dá)情況。結(jié)果:組織中PDCD5蛋白的陽性表達(dá)率為33.7l%(30/89)低于NCE組織的76%(19/25),PDCD5蛋白的陽性表達(dá)率與有無淋巴結(jié)轉(zhuǎn)移極顯著相關(guān)(P<0.001),與病理分級低分化和高分化顯著相關(guān)(尸<0.05)。組織中Bcl.2蛋白的陽性表達(dá)率為67.42%(60/89)高于NCE組織的40%(10/25),Bcl.2蛋白的陽性表達(dá)率與宮頸癌的浸潤深度極顯著相關(guān)(氏0.001),與腫瘤的病理分級及有無淋巴結(jié)轉(zhuǎn)移非常顯著相關(guān)(P<O.01)。組織中Bcl.2和PDCD5蛋白表達(dá)呈負(fù)相關(guān)(產(chǎn)一0.427,P<O.01);藍(lán)萼甲素對SiHa細(xì)胞有抑制增殖效應(yīng),且呈劑量及時(shí)間依賴趨勢;Hoechst 33258染色后,熒光顯微鏡下見SiHa細(xì)胞的胞核致密濃染,或里碎塊狀;TEM觀察顯示SiHa細(xì)胞出現(xiàn)細(xì)胞核皺縮,染色質(zhì)邊集等細(xì)胞凋亡的特征性改變;FCM檢測結(jié)果,隨著時(shí)間的延長,SiHa細(xì)胞凋亡率逐漸增加且S期細(xì)胞比例也逐漸增加;RT-PCR結(jié)果顯示Bcl.2 mRNA的表達(dá)下調(diào),PDCD5、Caspase.3、Caspase.9、p5 3、BaxmRNA的表達(dá)上調(diào);Western blot結(jié)果顯示Bcl.2蛋白表達(dá)下調(diào),PDCD5和Bax蛋白表達(dá)上調(diào)。結(jié)論:PDCD5、Bcl.的發(fā)生發(fā)展有一定的相關(guān)性,可以中文摘要PDCI)5、Bcl-2蛋白在富頸鱗癌中的表達(dá)及藍(lán)萼甲素對宮頸鱗癌SiHa細(xì)胞的絲用機(jī)制探討——●_●●__●●__—____●■_-_■■-■__●--__-●●____。______-____■______。。。。。。。。。。。。’。。-’’’�！�。。�！�1。。。。。。。�！�1。。。�！瓙盒猿潭鹊闹笜�(biāo);藍(lán)萼甲素對體外培養(yǎng)的SiHa細(xì)胞增殖具有顯著的抑制作用,該作用主要與誘導(dǎo)SiHa細(xì)胞凋亡的同時(shí)使其細(xì)胞周期阻滯于S期有關(guān),其分子機(jī)制可能是激活線粒體凋亡途徑。關(guān)鍵j夏"]:PDCD5;Bcl.2;宮頸鱗癌;藍(lán)萼甲素;SiHa細(xì)胞;凋亡n作者:夏維指導(dǎo)教師:王修珍副教授顧振綸教授蔣小崗副教授郭次儀教授PDCD5、Bcl-2蛋白在宮頸鱗癌中的表達(dá)及藍(lán)萼甲素對宮頸鱗癌SiHa細(xì)胞的作用機(jī)制探討英文摘要Expressions of PDCD5 and Bcl--2 proteins in cervicalsquamous cell carcinoma and approach of mechanism’of Glaucocalyxin A on SiHa cell lineAbstractAim To examine the expressions of PDCD5 and Bcl-2 in cervical squamous eellcarcinoma()tissues.To study the antiprolifrative effects and the underlingmechanisms of Glaucocalyxin A on human cervical carcinoma SiHa cells.Methods 89 tissue blocks and 25 normal cervical epithelium(NCE)tissueblocks were analyzed by immunohistochemistry for PDCD5 and Bcl一2 proteins.Theantiprolifrative effect of Glaucocalyxin A on SiHa cells was measured by MTT assay.The morphological changes were observed by Hoechst33258 fluorochrome staining andtransmission electron microscope(TEM).The rates of cell cycle distribution andapoptosis were analyzed by flow cytometry.The molecular mechanisms involved in theeffects ofGlaucocalyxin Aon SiHa cells were studied by RT-PCR and Western blot.Results There were significant differences of PDCD5 expression between tissues(33.71%,30/89)and NCE tissues(76%,19/25).The expression rate Wasassociated wim pathological grade(P<0.05)and lymph node metastasis(P<0.00 1).There were also significant differences of Bcl-2 expression between tissues(67.42%,60/89)and NCE tissues(40%,l o/25).The expression rate Was associated withpathological grade(P<O.O 1)and infiltration depth(P<0.OO1).The expressions ofPDCD5 protein were negatively related to those of Bcl-2 protein in tissues(r---0.427,P<0.01).The growth potential of human cervical carcinoma SiHacells was siginificantly inhibited by Glaucocalyxin A.Apoptosis morphological changeswere observed on SiHa cells after Glaucocalyxin A treatment by Hoechst 33258fluorochrome staining and TEM.“Sub-Ol’’phase peak Was appeared and cell cycle Wasarrested in S phase after Glaucocalyxin A treatment.The expression levels of PDCD5、p53、Bax、Caspase-3 and Caspase一9 mRNA were upregulated and the expression levelof bcl·2 mRNA was downregulated.The expression levels of PDCD5 and Baxproteinswere upregulated and the expression level of Bcl-2 protein was downregulated.英文摘要PDCD5、墅絲量魚壟宣堡壁墮主箜壅姿墾堇蔓!耋墮宣堡箜墮璺!望苧塑墮笪堡旦墊趔堡墮二二=二_=二—■二————-_·__——————————————————————————————————————。——————————————————一Conclusions PDCD5 and Bcl.2 Can serve as a biomarker to predict the malignantdegree of .The antiproliferative effects of Glaucocalyxin A on human cervicalcarcinoma SiHa cells may be associated with apopl:osis and S phase cell cycle arrestthrough Mitochondrial apoptosis pathway.Keywords:PDCD5;Bcl.2;cervical carcinoma;Glaucocalyxin A;SiHa cells;apoptosisWritten by:Wei XiaSupervised by:Prof.Xiuzhen WangProf.Zhenlun GuProf.Xiaogang JiangProf.Chiyi Kwok

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  本文關(guān)鍵詞：香港保健協(xié)會資助項(xiàng)目，由筆耕文化傳播整理發(fā)布。