本文關(guān)鍵詞：雞論文，由筆耕文化傳播整理發(fā)布。

網(wǎng)友xxj16588近日為您收集整理了關(guān)于大骨雞論文：堿性磷酸酶的提取及在線固定化研究的文檔，，希望對(duì)您的工作和學(xué)習(xí)有所幫助。以下是文檔介紹：大骨雞論文:堿性磷酸酶的提取及在線固定化研究【中文摘要】堿性磷酸酶廣泛存在于動(dòng)物、植物、微生物中,直接參與磷酸基團(tuán)的轉(zhuǎn)移和代謝過程。固定化堿性磷酸酶可以作為分子識(shí)別元件,進(jìn)行多種農(nóng)藥、重金屬離子的檢測(cè)。順序注射系統(tǒng)在固定化酶在線制備方面具有巨大潛能。在此基礎(chǔ)上,本論文進(jìn)行了以下四方面的研究:1.莊河大骨雞骨骼粗壯,其生長(zhǎng)發(fā)育與成骨細(xì)胞的快速分化關(guān)系密切,而成骨細(xì)胞的分化又是在ALP作用下進(jìn)行。本論文以大骨雞肝作為酶源,分別采用有機(jī)溶劑沉淀法和鹽析法提取ALP粗酶液,考察了ALP的催化動(dòng)力學(xué)性質(zhì)。結(jié)果表明:兩種方法提取的ALP單位質(zhì)量比活力相近;ALP粗酶液催化磷酸苯二鈉的最適溫度為500C,最適酸度為10.8,米氏常數(shù)為Km=1.59mmol/L。Pb2+、Ba2+對(duì)ALP活力有抑制作用,Ca2+對(duì)ALP活力有激活作用。2.以XC-72/PVC復(fù)合材料作為載體,順序注射系統(tǒng)-固相微柱-紫外可見吸收光譜系統(tǒng)為操作平臺(tái),對(duì)大骨雞肝ALP進(jìn)行在線固定化研究,并比較固定化ALP與游離ALP的理化性質(zhì)。結(jié)果表明:與游離ALP相比,固定化ALP適宜溫度和酸度范圍范圍均擴(kuò)大,分別為50℃~60℃及pH9.0~10;對(duì)底物的親和性增強(qiáng),米氏常數(shù)為Km=0.125mmol/L;Ca2+濃度在0.9mmol/L以下對(duì)固定化ALP活力有激活作用,1.0mmol/L以上為抑制作用。3.分別采用戊二醛交聯(lián)的殼聚糖和復(fù)合材料XC-72/PVC對(duì)大腸桿菌ALP進(jìn)行了固定化,以生色底物磷酸苯二鈉和生熒底物4-***傘形***磷酸酯考察了固定化ALP對(duì)底物的親和力,同時(shí)考察了載體材料對(duì)兩種底物的吸附情況。結(jié)果表明:固定化ALP對(duì)磷酸苯二鈉的親和力強(qiáng)于對(duì)4-MUP;戊二醛修飾的殼聚糖對(duì)生熒底物和對(duì)應(yīng)產(chǎn)物的吸附作用顯著,而XC-72/PVC僅對(duì)生熒產(chǎn)物吸附嚴(yán)重;兩種材料對(duì)生色底物均無吸附作用,對(duì)產(chǎn)物略有吸附。4.采用戊二醛交聯(lián)的殼聚糖作為載體,考察了ALP在殼聚糖表面的在線共價(jià)偶聯(lián)性能,結(jié)果表明:利用共價(jià)交聯(lián)法在線制備的固定化ALP活力明顯低于物理吸附法制備的固定化ALP活力,且固定化ALP的穩(wěn)定性良好,說明在順序注射系統(tǒng)中利用共價(jià)偶聯(lián)法在線固定化ALP有待進(jìn)一步探討�！居⑽恼緼lkalinephosphataseexistswidelyinanimals,plantsandmicrobes,itdirectlyparticipateinphosphoricacidgroupsandmetabolicprocesses.Immobilized-ponentstodetectingpesticidesandheavymetalions.Thereistremendouspotentialofthisstudyonemployingsequentialinjectionsystemtoonline-immobilizedenzyme.Onthebasis,pletedthefoursortsofworkasthefollowing:PartIThebig-bonechickenboneisbrawny,itsgrowthrelateclosedtotherapiddifferentiationofosteoblasts,howevertheALPactiveinthedifferentiationofosteoblasts.Inthisthesis,anicsolventprecipitationandsalting-outmethodswereusedtoextractALPfrombig-bonechickenliver,icpropertiesoftheextractedALPwerealsostudied.TheresultsindicatedthattheoptimumpHandtemperaturefortheextractedALPtocatalyzethephenylphosphoricaciddisodiumis10.8and50℃respectively,theMichaelis-Mentenconstant(Km)is1.59mmol/L.Pb+isaninhibitortoALP,Ba2+isanactivator,andCa2+isanactivator.PartIIEmployingasequentialinjectionsystemtoonline-positematerialXC-72/PVCascarrier,thenthepropertiesofthefreeALPandimmobilized-paredtostudy.TheresultsindicatedthattherangeofoptimumpHandtemperaturefortheimmobilized-ALPareallexpand,theappropriatetemperaturerangeoftheimmobilized-ALPis50~60℃,andthesuitableacidityrangeis9.0-10,theMichaelis-Mentenconstantis0.125mmol/L.Ca2+haveactivatedfunctiontotheimmobilized-ALPwhentheconcentrationisfollowing0.9mmol/L,butasaninhibitionwhentheconcentrationmorethan1.0mmol/L.positematerialsXC-72/PVCwereusedtoimmobiliztedtheALPrespectively.Theaffinityofsubstrateandimmobilized-ALPwerestudiedrespectively,usingthedisodiumphenylphosphatehydrateasthephenacylmethylenesubstrateand4-methylumbelliferylphosphateasthefluorescencesubstrate.Theadsorptionwasinvestigatedbetweenthecarriersandthesubstrates.Theresultsindicatedthattheaffinityofimmobilized-ALPtophosphoricacidsodiumbenzodiazepinesisbetterthanthe4-MUP,thechitosanmodifiedbyglutaraldehydehasasignificantlyadsorptiontothe4-MUPandthecatalyzeproduct,whileXC-72/PVConlyhasaseriousadsorptiontothefluorescenceproduct.Twokindsofmaterialhavenoattractiont

12>

  本文關(guān)鍵詞：雞論文，由筆耕文化傳播整理發(fā)布。