【摘要】：本研究以普通型野大豆和耐鹽型野大豆為實驗材料,采用沙土培養(yǎng)的方式,比較研究了中、堿性鹽脅迫下兩種生態(tài)型野大豆幼苗葉片中代謝物的變化。實驗通過分析普通型野大豆和耐鹽型野大豆響應(yīng)中、堿性鹽脅迫的代謝圖譜來揭示野大豆的耐鹽機制。實驗結(jié)果顯示,在兩種鹽脅迫的處理下,耐鹽型野大豆幼苗的生長所受到的脅迫抑制明顯小于普通型野大豆。差異代謝物的圖譜分析顯示,兩種鹽脅迫處理下,普通型野大豆幼苗葉片中可溶性糖和脂肪酸含量的降低程度顯著高于耐鹽型野大豆。這些代謝物包括乳糖、核糖、月桂酸、棕櫚酸、硬脂酸以及亞麻酸。堿性鹽脅迫下,兩種生態(tài)型野大豆幼苗葉片中均有特異氨基酸的積累。這些氨基酸包括纈氨酸,酪氨酸,谷氨酸,亮氨酸和異亮氨酸。堿性鹽脅迫下,耐鹽型野大豆幼苗葉片中大部分有機酸含量以及脯氨酸含量均明顯升高。這些有機酸包括粘酸、戊二酸、半乳糖酸和脫氫抗壞血酸。普通型野大豆在響應(yīng)兩種鹽脅迫時TCA循環(huán)增強,但在耐鹽型野大豆中減弱。論文結(jié)論如下:一、鹽脅迫抑制野大豆幼苗的生長,根部生長受到抑制更為顯著;二、堿性鹽脅迫對野大豆幼苗生長的抑制更為強烈,耐鹽型野大豆表現(xiàn)出更強的適應(yīng)能力;三、普通型野大豆的耐鹽機制依賴于TCA循環(huán)的增強產(chǎn)生更多的ATP;耐鹽型野大豆更多依靠氨基酸和有機酸的代謝調(diào)節(jié)產(chǎn)生更多的相容性溶質(zhì)。本文基于代謝組學(xué)揭示了鹽脅迫下野大豆的耐鹽機制,為栽培大豆的高抗育種提供了豐富的理論基礎(chǔ),顯示出野大豆是栽培大豆遺傳改良的寶貴資源,同時預(yù)示著野大豆種質(zhì)資源的開發(fā)、利用和保護具有重大的意義。
[Abstract]:In this study, the changes of the metabolites in the leaves of two ecotypes of wild soybean seedlings under alkaline salt stress were studied by means of sand culture. The salt tolerance mechanism of wild soybean was revealed by analyzing the metabolic profile of basic salt stress in the response of common wild soybean and salt tolerant wild soybean. The experimental results show that under the treatment of two salt stress, the stress inhibition of the growth of the salt-resistant wild soybean seedlings is obviously lower than that of the common wild soybean. The results showed that the content of soluble sugar and fatty acid in the leaves of common wild soybean seedlings was significantly higher than that of salt-resistant wild soybean under two salt stress treatments. These metabolites include lactose, ribose, lauric acid, palmitic acid, stearic acid, and linolenic acid. The specific amino acid accumulation in the leaves of two ecotypes of wild soybean seedlings under alkaline salt stress. These amino acids include the amino acid, the tyrosine, the glutamic acid, the leucine and the isoleucine. Under alkaline salt stress, the content of most organic acids and the content of proline in the leaf of salt-resistant wild soybean seedlings were increased obviously. These organic acids include acid, glutaric, galactotic, and dehydroascorbic acid. In response to the two salt stress, the common wild soybean was enhanced in TCA cycle, but decreased in the salt-resistant wild soybean. The results of the paper are as follows:1. The salt stress inhibits the growth of the wild soybean seedlings, and the growth of the root is more obvious; secondly, the inhibition of the growth of the wild soybean seedlings by the alkaline salt stress is stronger, and the salt-tolerant wild soybean has stronger adaptability; and thirdly, The salt tolerance mechanism of the common wild soybean is dependent on the increase of the TCA cycle to produce more ATP, and the salt tolerant wild soybean is more dependent on the metabolic regulation of the amino acid and the organic acid to produce more compatible solute. The salt-tolerant mechanism of the wild soybean under salt stress is revealed based on metabolomics, which provides a rich theoretical basis for the high-resistance breeding of the cultivated soybean, and shows that the wild soybean is a valuable resource for the genetic improvement of the cultivated soybean, and also indicates the development of the wild soybean germplasm resources. And is of great significance to use and protect.
【學(xué)位授予單位】：東北師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S565.1

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