【摘要】：缺鎂(Mg)多發(fā)生在強(qiáng)酸性的沙質(zhì)土壤中,而柑橘主要生長(zhǎng)在酸性或強(qiáng)酸性的土壤當(dāng)中。缺鎂會(huì)降低一些柑橘園的果實(shí)品質(zhì)和產(chǎn)量�；诖�,我們率先采用Illumina sequencing對(duì)缺Mg和供Mg充足的雪柑根系構(gòu)成的2個(gè)RNA文庫(kù)進(jìn)行了高能量測(cè)序,旨在獲得一些可能與雪柑根系耐缺Mg相關(guān)的miRNA。通過(guò)高能量測(cè)序,本研究從缺Mg雪柑根系中分離得到了 101個(gè)上調(diào)和69個(gè)下調(diào)miRNA。分析結(jié)果表明,雪柑根系對(duì)缺Mg的適應(yīng)性響應(yīng)可能有以下幾個(gè)方面:(a)抑制根系呼吸,以及通過(guò)誘導(dǎo)miR158和miR2919的表達(dá)抑制呼吸相關(guān)基因的表達(dá);(b)通過(guò)下調(diào)相關(guān)miRNA的表達(dá)來(lái)提高其抗氧化系統(tǒng)(miR780、miR6190、miR1044、miR5261和miR1151)和對(duì)低磷的適應(yīng)性(miR6190);(c)通過(guò)改變miR6190、miR6485、miR1044、miR5029和miR3437來(lái)激活與運(yùn)輸相關(guān)基因的表達(dá);(d)降低miR394、miR1044、miR5261、iR1151和miR5029的表達(dá),從而提高蛋白泛素化;(e)通過(guò)下調(diào)miR5261和miR6485和上調(diào)miR158的表達(dá)來(lái)維持根部的生長(zhǎng);(f)通過(guò)調(diào)控miR5176和miR6485(miR6028、miR190、miR6485、miR5621、miR160和miR7708)啟動(dòng)DNA修復(fù)(轉(zhuǎn)錄調(diào)節(jié))。此外,與根信號(hào)傳導(dǎo)相關(guān)的一些缺Mg響應(yīng)miRNA也可能在雪柑對(duì)缺Mg的適應(yīng)性響應(yīng)中起作用。缺Mg響應(yīng)miRNA可能在根信號(hào)傳導(dǎo)中起作用,從而提高雪柑對(duì)缺Mg適應(yīng)性。總之,本研究分離鑒定了一些與缺Mg耐性相關(guān)的新的缺Mg響應(yīng) miRNA(miR5261、miR158、miR6190、miR6485、miR1151 和 miR1044)。這些結(jié)果揭示了在植物營(yíng)養(yǎng)缺乏的適應(yīng)性方面miRNAs發(fā)揮作用的一些新的線索。
[Abstract]:Magnesium deficiency (Mg) mostly occurs in sandy soil with strong acidity, while citrus mainly grows in acid or strong acid soil. Magnesium deficiency reduces fruit quality and yield in some citrus orchards. Based on this, we first used Illumina sequencing to sequence two RNA libraries composed of Mg-deficient and Mg-rich roots of Xuegan, in order to obtain some miRNA. which may be related to Mg tolerance of Xuegan root. The purpose of this study is to obtain some miRNA. libraries which may be related to the Mg tolerance of Xuegan root. By high energy sequencing, 101 up-regulated and 69 down-regulated miRNA. were isolated from the roots of Mg-deficient snow orange. The results showed that the adaptive response of Xuegan root to Mg deficiency might have the following aspects: (a) inhibited root respiration and inhibited the expression of respiratory-related genes by inducing the expression of miR158 and miR2919. (b) enhanced its antioxidant system (miR780,miR6190,miR1044,miR5261 and miR1151) and its adaptation to low phosphorus by down-regulating the expression of associated miRNA (miR6190); (c) by changing miR6190,miR6485,miR1044,miR5029 and miR3437 to activate the expression of transport-related genes. (d) decreased the expression of miR394,miR1044,miR5261,iR1151 and miR5029, thus increased the expression of ubiquitin; (e) to maintain root growth by down-regulating the expression of miR5261 and miR6485 and up-regulating the expression of miR158. (f) initiates DNA repair (transcriptional regulation) by regulating miR5176 and miR6485 (miR6028,miR190,miR6485,miR5621,miR160 and miR7708). In addition, some lack of Mg response miRNA associated with root signal transduction may also play a role in the adaptive response of Xuegan to Mg deficiency. The lack of Mg response miRNA may play a role in root signal transduction, thus improving the adaptability of Xuequan to Mg deficiency. In conclusion, we isolated and identified some new Mg-deficient response miRNA (miR5261,miR158,miR6190,miR6485,miR1151 and miR1044) related to lack of Mg tolerance. These results reveal some new clues to the role of miRNAs in the adaptation of plant nutrition deficiency.
【學(xué)位授予單位】：福建農(nóng)林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：S666.1

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本文編號(hào)：2450935