本文關鍵詞：南瓜未授粉子房離體培養(yǎng)及植株再生，由筆耕文化傳播整理發(fā)布。

南瓜未授粉子房離體培養(yǎng)及植株再生

Studies of in Vitro Culture and Plant Regeneration of Unfertilized Ovary of Pumpkin

[1] [2] [3] [4] [5] [6]

Ziyang Min,Han Li,Tian Zou,Long Tong,Juan Cheng,Xiaowu Sun（1.College of Horticulture, Hunan Agricultural University, Changsha 410128, China; 2.Hunan Province Melon Research Institut

[1]湖南農業(yè)大學園藝園林學院,長沙410128; [2]湖南省瓜類研究所,邵陽422001; [3]湖南省西瓜甜瓜研究所,長沙410125

文章摘要：以南瓜（Cucurbita moschata）未授粉子房為外植體,在離體條件下研究了激素種類、基因型、胚囊發(fā)育時期、消毒方式和熱激處理時間對胚狀體誘導效果的影響,旨在建立完善的南瓜未授粉子房離體誘導再生體系。結果表明,MS＋4.0mg·L–1 2,4-D＋0.5 mg·L–1 NAA＋1.0 mg·L–1 6-BA和MS＋0.04 mg·L–1 TDZ兩種培養(yǎng)基胚狀體的誘導率都較高,分別為19.8%和20.1%,二者相比,使用TDZ誘導更加簡單;在6個南瓜品種中,2個生長勢較強的品種（雪峰蜜本和鱷魚南瓜）胚狀體誘導率較高,適合作為離體雌核誘導的實驗材料;開花當天的子房先切片后消毒可以有效降低愈傷組織的形成,提高胚狀體的誘導率;在黑暗且35°C條件下熱激5天有利于子房的轉綠和出胚。將子葉胚轉移至無激素的MS培養(yǎng)基上得到再生植株并移栽成活。經鑒定有7個再生植株的染色體數(shù)目n=x=22,氣孔保衛(wèi)細胞葉綠體數(shù)的平均值為4.28個,是單倍體植株。

Abstr：Using unfertilized ovaries of pumpkin as explants, we determined the effect of different combinations and concentrations of growth regulators, genotypes, embryo sac development stages, disinfection methods and duration of high-temperature pretreatments on embryoid induction via in vitro culture to establish a regeneration system of unfertilized ovaries of pumpkin. The media MS＋4.0 mg·L–1 2,4-D＋0.5 mg·L–1 NAA＋1.0 mg·L–1 6-BA and MS＋0.04 mg·L–1 TDZ achieved higher induction rates, 19.8% and 20.1%, respectively, as compared to other media examined, with the TDZ-induced method easier to operate. Among the 6 pumpkin varieties tested, two（Xue feng mi ben and E yu nan gua）, with stronger growth, had higher embryoid induction rates and were more suitable to be used as experiment materials. The operation of slicing and then sterilizing the ovaries picked on bloom day decreased the cavernous callus formation effectively and increased the embryoid induction rate. Treatments with darkness and heat shock（35°C） for 5 days could help ovaries turn green and induce embryoids. When moved to hormone-free MS medium, the cotyledon embryos developed to regenerated seedlings and survived after transplantation. Seven regenerated plantlets had chromosome number n=x=22 and an average number of chloroplasts in stomatal guard cells of 4.28, so they were haploid plants.

文章關鍵詞：

Keyword：:embryoid pumpkin regenerated seedling unfertilized ovary

課題項目：南瓜產業(yè)技術研究與示范（No.201303112）

作者信息：會員可見

  本文關鍵詞：南瓜未授粉子房離體培養(yǎng)及植株再生，由筆耕文化傳播整理發(fā)布。