基于微流控捕獲技術(shù)的孤立顆粒體散射函數(shù)研究
發(fā)布時(shí)間：2019-03-09 18:08
【摘要】：顆粒的光學(xué)體散射函數(shù)特性是顆粒的一項(xiàng)重要的物理學(xué)特性。傳統(tǒng)的顆粒體散射函數(shù)研究主要建立在使用顆粒群樣本間接測(cè)量的基礎(chǔ)上,現(xiàn)有的孤立顆粒體散射函數(shù)直接測(cè)量方法存在樣本適應(yīng)性差和易受環(huán)境因素干擾等缺點(diǎn)。因此,設(shè)計(jì)一種穩(wěn)定、可靠、快速、環(huán)境依賴少的孤立顆粒體散射函數(shù)測(cè)量系統(tǒng)具有十分重要的價(jià)值。本論文,從孤立顆粒樣本捕獲和體散射函數(shù)測(cè)量?jī)蓚�(gè)方向出發(fā),結(jié)合微流控芯片技術(shù),提出了基于流體力顆粒捕獲微流芯片的孤立顆粒體散射函數(shù)測(cè)量方法,解決了孤立顆粒體散射測(cè)量的核心問題。通過綜合運(yùn)用流體力學(xué)方法與有限元分析工具,設(shè)計(jì)并制備具有自調(diào)節(jié)能力的孤立顆粒捕獲微流控芯片；根據(jù)孤立顆粒光散射的特點(diǎn),設(shè)計(jì)了基于空間濾波器和PMT光電探測(cè)器的散射光接收裝置；基于各模塊設(shè)計(jì)成果,完成了孤立顆粒體散射函數(shù)測(cè)量系統(tǒng),通過對(duì)不同粒徑顆粒的實(shí)際測(cè)量和分析,驗(yàn)證了該系統(tǒng)用于孤立顆粒體散射函數(shù)研究的有效性和探測(cè)能力。本論文闡述了微流控芯片內(nèi)部流道中的流場(chǎng)分布特點(diǎn),通過建立流阻等效模型,運(yùn)用Matlab對(duì)流道結(jié)構(gòu)參數(shù)與流場(chǎng)分布特性關(guān)系進(jìn)行了綜合計(jì)算,提出了一種不僅可同時(shí)達(dá)到高捕獲效率和高捕獲準(zhǔn)確率,而且利用捕獲微粒自身對(duì)流體分布進(jìn)行自動(dòng)反饋調(diào)節(jié)的微流道結(jié)構(gòu)。運(yùn)用有限元流體計(jì)算工具,對(duì)前述結(jié)構(gòu)進(jìn)行三維仿真研究,并進(jìn)一步對(duì)影響捕獲效果的流場(chǎng)局部特征進(jìn)行優(yōu)化。在此基礎(chǔ)上設(shè)計(jì)并制備了具有高光學(xué)透過率的孤立顆粒捕獲芯片。為實(shí)現(xiàn)孤立顆粒散射光的有效探測(cè),通過原理分析、理論計(jì)算、數(shù)值仿真和綜合對(duì)比,設(shè)計(jì)并搭建了一套基于空間濾波器和PMT光電探測(cè)器的角度掃描式大動(dòng)態(tài)范圍散射光測(cè)量模塊,并實(shí)驗(yàn)驗(yàn)證了系統(tǒng)實(shí)際視場(chǎng)和大光強(qiáng)動(dòng)態(tài)范圍下的線性度。基于理論分析與上述孤立顆粒捕獲模塊、散射光測(cè)量模塊的研究成果,研制了一套孤立顆粒體散射函數(shù)測(cè)量系統(tǒng)。通過消除了有機(jī)溶液吸脹、環(huán)境溫度波動(dòng)和層流邊界對(duì)折射率匹配的影響,提高了光學(xué)匹配效果。通過空間精密對(duì)準(zhǔn),對(duì)實(shí)際捕獲的孤立聚苯乙烯顆粒的大角度范圍內(nèi)的體散射函數(shù)進(jìn)行實(shí)際測(cè)量。多次重復(fù)測(cè)量20.42μm顆粒在20至1620之間的散射光分布在光強(qiáng)變化約1×106范圍內(nèi)的波動(dòng)中心值約為20%-25%。直徑分別為23μm,23.75μm,31μm的聚苯乙烯標(biāo)準(zhǔn)顆粒樣品的實(shí)驗(yàn)結(jié)果與Mie散射理論曲線趨勢(shì)上符合良好。實(shí)驗(yàn)數(shù)據(jù)和理論曲線中20°至55°區(qū)間內(nèi)數(shù)據(jù)進(jìn)行曲線擬合和參數(shù)分析,相同粒徑顆粒的理論與實(shí)驗(yàn)擬合參數(shù)差別不大于8.6%；不同粒徑顆粒的擬合參數(shù)間差異明顯。通過對(duì)顆粒體散射函數(shù)的實(shí)際測(cè)量與分析,證明本方法在定性和定量角度均與理論曲線符合較好,是一種快速、有效且富有潛力的孤立顆粒體散射測(cè)量方法。
[Abstract]:The optical scattering function of particles is an important physical property of particles. The traditional research on particle scattering function is mainly based on the indirect measurement of particle group samples. The existing methods for direct measurement of isolated particle scattering function have some disadvantages such as poor sample adaptability and easy to be disturbed by environmental factors. Therefore, it is very important to design an isolated particle scattering function measurement system which is stable, reliable, fast and less dependent on the environment. In this thesis, based on the technique of microfluidic chip, an isolated particle scattering function measurement method based on fluid particle capture microfluidic chip is proposed, which is based on the capture of isolated particle samples and the measurement of volume scattering function, and the microfluidic chip technology is used to measure the scattering function of isolated particles. The core problem of scattering measurement of isolated particles is solved. An isolated particle capture microfluidic chip with self-adjusting ability was designed and fabricated by using fluid mechanics method and finite element analysis tools. According to the characteristics of isolated particle light scattering, a scattering light receiving device based on spatial filter and PMT photodetector is designed. Based on the design results of each module, an isolated particle scattering function measurement system is completed. Through the actual measurement and analysis of different particle sizes, the validity and detection ability of the system used in the study of scattering function of isolated particles are verified. In this paper, the characteristics of flow field distribution in the internal channel of microfluidic chip are described. By establishing the equivalent model of flow resistance, the relationship between the structural parameters of the channel and the distribution characteristics of the flow field is comprehensively calculated by using Matlab. This paper presents a micro-channel structure which not only can achieve high capture efficiency and high capture accuracy at the same time, but also uses the captured particles to adjust the fluid distribution automatically. The three-dimensional simulation of the above-mentioned structure is carried out by using the finite element fluid calculation tool, and the local characteristics of the flow field affecting the capture effect are further optimized. On this basis, an isolated particle capture chip with high optical transmittance was designed and fabricated. In order to realize the effective detection of isolated particle scattering light, through the principle analysis, theoretical calculation, numerical simulation and comprehensive comparison, A set of angular scanning large dynamic range scattering light measurement module based on spatial filter and PMT photodetector is designed and built. The linearity of the system in real field of view and large dynamic range of light intensity is verified by experiments. Based on the theoretical analysis, above-mentioned solitary particle capture module and scattering light measurement module, a set of scattering function measurement system for isolated particles is developed. The optical matching effect is improved by eliminating the effects of organic solution imbibition, ambient temperature fluctuation and laminar boundary on refractive index matching. The volume scattering function in the wide angle range of the isolated polystyrene particles captured in practice is measured by space precision alignment. The scattering light distribution of 20.42 渭 m particles in the range of 20 to 1620 is measured repeatedly. The center value of the scattering light distribution in the range of 1 脳 10 ~ 6 is about 20% ~ 25%. The experimental results of polystyrene standard particles with diameters of 23 渭 m, 23. 75 渭 m and 31 渭 m are in good agreement with the theoretical curve of Mie scattering. The curve fitting and parameter analysis between the experimental data and the theoretical data in the range of 20 擄to 55 擄show that the theoretical and experimental fitting parameters of the same particle size are less than 8.6%, and the fitting parameters of different particle sizes are obviously different. Through the actual measurement and analysis of particle scattering function, it is proved that this method is a fast, effective and potential method for measuring the scattering of isolated particles from both qualitative and quantitative angles in good agreement with the theoretical curve.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2016
【分類號(hào)】：TN492
本文編號(hào)：2437728
[Abstract]:The optical scattering function of particles is an important physical property of particles. The traditional research on particle scattering function is mainly based on the indirect measurement of particle group samples. The existing methods for direct measurement of isolated particle scattering function have some disadvantages such as poor sample adaptability and easy to be disturbed by environmental factors. Therefore, it is very important to design an isolated particle scattering function measurement system which is stable, reliable, fast and less dependent on the environment. In this thesis, based on the technique of microfluidic chip, an isolated particle scattering function measurement method based on fluid particle capture microfluidic chip is proposed, which is based on the capture of isolated particle samples and the measurement of volume scattering function, and the microfluidic chip technology is used to measure the scattering function of isolated particles. The core problem of scattering measurement of isolated particles is solved. An isolated particle capture microfluidic chip with self-adjusting ability was designed and fabricated by using fluid mechanics method and finite element analysis tools. According to the characteristics of isolated particle light scattering, a scattering light receiving device based on spatial filter and PMT photodetector is designed. Based on the design results of each module, an isolated particle scattering function measurement system is completed. Through the actual measurement and analysis of different particle sizes, the validity and detection ability of the system used in the study of scattering function of isolated particles are verified. In this paper, the characteristics of flow field distribution in the internal channel of microfluidic chip are described. By establishing the equivalent model of flow resistance, the relationship between the structural parameters of the channel and the distribution characteristics of the flow field is comprehensively calculated by using Matlab. This paper presents a micro-channel structure which not only can achieve high capture efficiency and high capture accuracy at the same time, but also uses the captured particles to adjust the fluid distribution automatically. The three-dimensional simulation of the above-mentioned structure is carried out by using the finite element fluid calculation tool, and the local characteristics of the flow field affecting the capture effect are further optimized. On this basis, an isolated particle capture chip with high optical transmittance was designed and fabricated. In order to realize the effective detection of isolated particle scattering light, through the principle analysis, theoretical calculation, numerical simulation and comprehensive comparison, A set of angular scanning large dynamic range scattering light measurement module based on spatial filter and PMT photodetector is designed and built. The linearity of the system in real field of view and large dynamic range of light intensity is verified by experiments. Based on the theoretical analysis, above-mentioned solitary particle capture module and scattering light measurement module, a set of scattering function measurement system for isolated particles is developed. The optical matching effect is improved by eliminating the effects of organic solution imbibition, ambient temperature fluctuation and laminar boundary on refractive index matching. The volume scattering function in the wide angle range of the isolated polystyrene particles captured in practice is measured by space precision alignment. The scattering light distribution of 20.42 渭 m particles in the range of 20 to 1620 is measured repeatedly. The center value of the scattering light distribution in the range of 1 脳 10 ~ 6 is about 20% ~ 25%. The experimental results of polystyrene standard particles with diameters of 23 渭 m, 23. 75 渭 m and 31 渭 m are in good agreement with the theoretical curve of Mie scattering. The curve fitting and parameter analysis between the experimental data and the theoretical data in the range of 20 擄to 55 擄show that the theoretical and experimental fitting parameters of the same particle size are less than 8.6%, and the fitting parameters of different particle sizes are obviously different. Through the actual measurement and analysis of particle scattering function, it is proved that this method is a fast, effective and potential method for measuring the scattering of isolated particles from both qualitative and quantitative angles in good agreement with the theoretical curve.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2016
【分類號(hào)】：TN492
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