基于納米復(fù)合材料的生物傳感器的研制及性能分析
發(fā)布時間：2018-12-06 20:06
【摘要】：電致化學(xué)發(fā)光生物傳感器具有很高的靈敏度,并且具有儀器設(shè)備簡單、操作方便、快捷、線性響應(yīng)范圍寬和易于實現(xiàn)自動化等顯著優(yōu)點,因此該新型傳感器越來越受到研究者們的廣泛關(guān)注。本文開展了針對三種不同納米復(fù)合材料的生物傳感器的優(yōu)化、制備和組裝,并且分別應(yīng)用于人體肝癌細(xì)胞的檢測之中,實現(xiàn)了對癌細(xì)胞的靈敏檢測,為生物醫(yī)學(xué)領(lǐng)域中的臨床檢測提供了新的平臺。本文開展了如下幾個方面的工作:利用CFD方法,通過Fluent軟件進(jìn)行模擬計算并優(yōu)化結(jié)構(gòu)設(shè)計,以光刻法為基礎(chǔ),以PDMS為原料,成功地設(shè)計和制備出具有不同結(jié)構(gòu)的微流控芯片。基于模擬數(shù)據(jù)得到的最優(yōu)條件,其中最優(yōu)條件包括芯片的最優(yōu)結(jié)構(gòu)(直通道,入射角度為120°),以及流速為50μL/min等。利用氨水和硝酸鈰溶液之間的化學(xué)反應(yīng),基于軟件模擬計算的最優(yōu)條件,本文將以上兩種溶液分別注入到微流控芯片中,開展二氧化鈰納米材料的合成,以及二氧化鈰納米材料形貌的控制。通過條件溫度,流速等外界參數(shù),實現(xiàn)了對產(chǎn)物二氧化鈰納米材料形貌的均勻可控。并基于此,利用離子層吸附法制備出硫化鎘包覆的二氧化鈰納米粒子,用于組裝電致化學(xué)發(fā)光傳感器。該組裝中,利用鉑絲電極為基底,利用層層組裝法實現(xiàn)了電致化學(xué)發(fā)光傳感器的制備,并應(yīng)用于人體肝癌細(xì)胞的檢測之中。其檢測范圍是1000-5000細(xì)胞每毫升,檢測限為1210個細(xì)胞。鑒于基于二氧化鈰-硫化鎘納米復(fù)合材料的生物傳感器的檢測范圍較窄,難以實現(xiàn)在臨床檢測中應(yīng)用的難題,所以,亟需制備新型納米復(fù)合材料的生物傳感器。鑒于二氧化硅的無毒性,以及良好的供電子能力,本文展開了合成納米二氧化硅材料的實驗。首先,利用溶液法和微流控方法分別制備出二氧化硅納米粒子,篩選出具有均一形貌的納米粒子,并利用離子層吸附法制備出二氧化硅-硫化鎘納米復(fù)合材料。紫外可見光譜、能譜,以及掃描電子顯微鏡等儀器被用來確認(rèn)該材料成功的被制備出來。通過利用鉑絲電極為基底,利用層層組裝法再次實現(xiàn)了電致化學(xué)發(fā)光傳感器的制備,并應(yīng)用于癌細(xì)胞的檢測之中。其檢測范圍是300-14000細(xì)胞每毫升。通過評估該傳感器的性能,發(fā)現(xiàn)該新式傳感器具有良好的穩(wěn)定性,選擇性和重現(xiàn)性。能夠用于癌癥早期臨床檢測的需要,是一種理想的新型檢測平臺。鑒于上述工作中制備的新型傳感器都需要進(jìn)行封裝工序,而不能夠直接進(jìn)行檢測,本文開發(fā)出一種利用泡沫鎳為基底的無需封裝的新型生物傳感器,并應(yīng)用于人體肝癌細(xì)胞的檢測之中。首先,通過對泡沫鎳表面的化學(xué)修飾改性,實現(xiàn)了泡沫鎳的親疏水性改變,紫外可見光譜,能譜,以及掃描電子顯微鏡等儀器被用來確認(rèn)該材料成功的被制備出來。然后利用層層組裝法制備出電致化學(xué)發(fā)光傳感器,并應(yīng)用于肝癌細(xì)胞的檢測之中。其檢測范圍是200-10000細(xì)胞每毫升,檢測限位78個細(xì)胞。通過評估該傳感器的性能,發(fā)現(xiàn)其具有良好的選擇性和重現(xiàn)性。但是通過多次檢測發(fā)現(xiàn),由于未經(jīng)過封裝,其穩(wěn)定性相比于封裝過的二氧化硅-硫化鎘納米復(fù)合材料的生物傳感器要略差。
[Abstract]:The electro-chemiluminescence biosensor has a high sensitivity, and has the remarkable advantages of simple instrument, convenient operation, rapidness, wide linear response range and easy realization of automation, so that the novel sensor is more and more widely concerned by the researchers. The invention has carried out the optimization, preparation and assembly of the biosensor for three different nano-composite materials, and is respectively applied to the detection of human liver cancer cells, and the sensitive detection of the cancer cells is realized, and a new platform is provided for clinical detection in the field of biomedicine. This paper has carried out the work of the following aspects: using the CFD method, the Fluent software is used to simulate and design the structure, based on the photolithography method, PDMS is used as the raw material, and the micro-flow control chip with different structure is successfully designed and prepared. The optimal conditions are obtained based on the simulation data, wherein the optimal conditions include the optimal structure of the wafer (straight pass, incident angle of 120 deg.), and flow rate of 50. m u.L/ min, etc. By means of the chemical reaction between the ammonia and the nitric acid solution, based on the optimal conditions of the software simulation, the above two solutions are respectively injected into the micro-flow control chip to carry out the synthesis of the double-oxidized nano-material and the control of the morphology of the two-oxidation-type nano-material. Through the external parameters such as the condition temperature, the flow rate and the like, the uniform and controllable morphology of the product binary oxide nano-material is realized. and based on the method, an ion-layer adsorption method is utilized to prepare the copper sulfide-coated dioxide nano-particles, and is used for assembling an electric-induced chemiluminescence sensor. in the assembly, a platinum wire electrode is used as a substrate, and the preparation of the electro-induced chemiluminescence sensor is realized by a layer-by-layer assembly method, and is applied to the detection of human liver cancer cells. The detection range is 1000-5000 cells per milliliter and the detection limit is 1210 cells. In view of the narrow detection range of the biosensor based on the dioxide-sulfide-sulfide nanocomposite, it is difficult to realize the problem of the application in clinical detection, and therefore, there is an urgent need to prepare a biosensor for a novel nanocomposite. In view of the non-toxicity of the silicon dioxide and the good electron supply capacity, the experiment of the synthesis of the nano-silica material is carried out. firstly, the silicon dioxide nano-particles are prepared by the solution method and the micro-flow control method, and the nano-particles with uniform morphology are screened out, and the silicon dioxide-copper sulfide nano composite material is prepared by the ion layer adsorption method. An instrument such as an ultraviolet visible spectrum, an energy spectrum, and a scanning electron microscope was used to confirm that the material was successfully prepared. By using the platinum wire electrode as the substrate, the preparation of the electro-induced chemiluminescence sensor is realized again by the layer-by-layer assembly method, and the method is applied to the detection of cancer cells. The range of detection is 300-14000 cells per milliliter. By evaluating the performance of the sensor, it is found that the new type of sensor has good stability, selectivity and reproducibility. can be used for early clinical detection of cancer, and is an ideal novel detection platform. In view of the fact that the new type of sensors prepared in the above-mentioned work need to be packaged, and the detection can not be carried out directly, a new type of biosensor based on foamed nickel as a substrate is developed and is applied to the detection of human liver cancer cells. First, by modifying the chemical modification of the surface of the foam nickel, the hydrophobic property change of the foam nickel, the ultraviolet visible spectrum, the energy spectrum, and the scanning electron microscope are used to confirm that the material is successfully prepared. and then the electro-induced chemiluminescence sensor is prepared by a layer-by-layer assembly method, and is applied to the detection of the liver cancer cells. The detection range is 200-10000 cells per milliliter, and the detection limit of 78 cells is detected. By evaluating the performance of the sensor, it was found to have good selectivity and reproducibility. but by multiple tests it is found that the stability is slightly less than that of the encapsulated silica-sulphide nanocomposite due to the fact that the package is not packaged.
【學(xué)位授予單位】：哈爾濱理工大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2016
【分類號】：TP212.3;TB383.1
本文編號：2366582
[Abstract]:The electro-chemiluminescence biosensor has a high sensitivity, and has the remarkable advantages of simple instrument, convenient operation, rapidness, wide linear response range and easy realization of automation, so that the novel sensor is more and more widely concerned by the researchers. The invention has carried out the optimization, preparation and assembly of the biosensor for three different nano-composite materials, and is respectively applied to the detection of human liver cancer cells, and the sensitive detection of the cancer cells is realized, and a new platform is provided for clinical detection in the field of biomedicine. This paper has carried out the work of the following aspects: using the CFD method, the Fluent software is used to simulate and design the structure, based on the photolithography method, PDMS is used as the raw material, and the micro-flow control chip with different structure is successfully designed and prepared. The optimal conditions are obtained based on the simulation data, wherein the optimal conditions include the optimal structure of the wafer (straight pass, incident angle of 120 deg.), and flow rate of 50. m u.L/ min, etc. By means of the chemical reaction between the ammonia and the nitric acid solution, based on the optimal conditions of the software simulation, the above two solutions are respectively injected into the micro-flow control chip to carry out the synthesis of the double-oxidized nano-material and the control of the morphology of the two-oxidation-type nano-material. Through the external parameters such as the condition temperature, the flow rate and the like, the uniform and controllable morphology of the product binary oxide nano-material is realized. and based on the method, an ion-layer adsorption method is utilized to prepare the copper sulfide-coated dioxide nano-particles, and is used for assembling an electric-induced chemiluminescence sensor. in the assembly, a platinum wire electrode is used as a substrate, and the preparation of the electro-induced chemiluminescence sensor is realized by a layer-by-layer assembly method, and is applied to the detection of human liver cancer cells. The detection range is 1000-5000 cells per milliliter and the detection limit is 1210 cells. In view of the narrow detection range of the biosensor based on the dioxide-sulfide-sulfide nanocomposite, it is difficult to realize the problem of the application in clinical detection, and therefore, there is an urgent need to prepare a biosensor for a novel nanocomposite. In view of the non-toxicity of the silicon dioxide and the good electron supply capacity, the experiment of the synthesis of the nano-silica material is carried out. firstly, the silicon dioxide nano-particles are prepared by the solution method and the micro-flow control method, and the nano-particles with uniform morphology are screened out, and the silicon dioxide-copper sulfide nano composite material is prepared by the ion layer adsorption method. An instrument such as an ultraviolet visible spectrum, an energy spectrum, and a scanning electron microscope was used to confirm that the material was successfully prepared. By using the platinum wire electrode as the substrate, the preparation of the electro-induced chemiluminescence sensor is realized again by the layer-by-layer assembly method, and the method is applied to the detection of cancer cells. The range of detection is 300-14000 cells per milliliter. By evaluating the performance of the sensor, it is found that the new type of sensor has good stability, selectivity and reproducibility. can be used for early clinical detection of cancer, and is an ideal novel detection platform. In view of the fact that the new type of sensors prepared in the above-mentioned work need to be packaged, and the detection can not be carried out directly, a new type of biosensor based on foamed nickel as a substrate is developed and is applied to the detection of human liver cancer cells. First, by modifying the chemical modification of the surface of the foam nickel, the hydrophobic property change of the foam nickel, the ultraviolet visible spectrum, the energy spectrum, and the scanning electron microscope are used to confirm that the material is successfully prepared. and then the electro-induced chemiluminescence sensor is prepared by a layer-by-layer assembly method, and is applied to the detection of the liver cancer cells. The detection range is 200-10000 cells per milliliter, and the detection limit of 78 cells is detected. By evaluating the performance of the sensor, it was found to have good selectivity and reproducibility. but by multiple tests it is found that the stability is slightly less than that of the encapsulated silica-sulphide nanocomposite due to the fact that the package is not packaged.
【學(xué)位授予單位】：哈爾濱理工大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2016
【分類號】：TP212.3;TB383.1
【參考文獻(xiàn)】
相關(guān)博士學(xué)位論文 前2條
1 陳章;新型生物傳感器的構(gòu)建及其在環(huán)境和生物檢測中的應(yīng)用研究[D];湖南大學(xué);2012年
2 莊儉;微注塑成型充模流動理論與工藝試驗研究[D];大連理工大學(xué);2007年
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