吡蟲啉和多菌靈分子印跡聚合物的制備及其在水果農(nóng)藥殘留檢測中的應(yīng)用
發(fā)布時間：2023-04-12 00:25
水果在生產(chǎn)過程中病蟲害種類多,發(fā)生頻繁。殺蟲劑吡蟲啉和殺菌劑多菌靈在果樹上應(yīng)用廣泛,其大量使用會產(chǎn)生農(nóng)藥殘留等系列問題,因此,農(nóng)殘檢測對保護(hù)消費者健康和保證食品質(zhì)量安全監(jiān)管至關(guān)重要。樣品前處理技術(shù)是農(nóng)藥殘留檢測的重要基礎(chǔ),隨著監(jiān)管力度的加大,我國以及一些發(fā)達(dá)國家,農(nóng)藥的最大殘留量(MRLs)值不斷降低,痕量農(nóng)藥的檢測需要更高效的前處理提取技術(shù)。分子印跡聚合物(MIPs)具有特異性結(jié)合力強(qiáng)、熱化學(xué)穩(wěn)定性好、可重復(fù)利用、靈敏度高、基質(zhì)干擾小等優(yōu)點。本文分別以甲基丙烯酰氯和β-環(huán)糊精為功能單體,制備了兩種分子印跡聚合物,并將其應(yīng)用于水果中吡蟲啉和多菌靈殘留的檢測。以吡蟲啉為模板,甲基丙烯酰氯為功能單體,采用表面印跡法合成了核殼型磁性分子印跡聚合物,并對其進(jìn)行了表征。表征結(jié)果表明,該磁性分子印跡聚合物具有特異質(zhì)結(jié)構(gòu)和良好的磁性。用擬二級動力學(xué)模型對等溫結(jié)合實驗進(jìn)行了評估,結(jié)果顯示其較好的符合Freundlich等溫方程。該聚合物對吡蟲啉的吸附量為5.75mg g-1,表明其具有良好的選擇性提取能力。另外,該聚合物在重復(fù)使用5次后,性能沒有顯著性的減弱。然后,利用該分子印跡...
【文章頁數(shù)】：130 頁
【學(xué)位級別】：博士
【文章目錄】：
摘要
abstract
Chapter 1 Introduction
1.1 Importance and concept
1.2 Magnetic molecular imprinting polymers
1.3 Mechanism of molecularly imprinted process
1.3.1 Basic approach
1.3.2 Major components of MIPs synthesis
1.3.2.1 Template
1.3.2.2 Functional monomer
1.3.2.3 Cross-linkers
1.3.2.4 Porogenic solvents
1.3.2.5 Initiators
1.4 Basic polymerization approaches
1.5 Challenges and limitations of MIPs application in pesticide detection
1.6 Perceptive Imprinting strategies
1.6.1 Innovative technologies for MIPs
1.6.1.1 Nanoimprinting technology
1.6.1.2 Living/controlled radical polymerization technology
1.6.1.3 Hollow porous polymer synthesis technology
1.6.1.4 Click chemistry cycloaddition reaction technology
1.6.1.5 Microfluidic on-line synthesis technology
1.6.2 Special Imprinting strategies
1.6.2.1 Multi-template imprinting strategy
1.6.2.2 Multi-functional monomer imprinting strategy
1.6.2.3 Dummy imprinting strategy
1.6.2.4 Segment imprinting strategy
1.6.2.5 Composite imprinting material strategy
1.6.3 Stimuli-responsive Imprinting Technologies
1.6.3.1 Photo-responsive technology
1.6.3.2 p H-Responsive technology
1.6.3.3 Dual/multi responsive technology
1.6.3.4 Other responsive technologies
1.7 Physicochemical characterization of MIPs
1.8 Application of MIPs in pesticides residues detection
1.8.1 Organophosphorus pesticides(OPPs)
1.8.2 Triazines
1.8.3 Carbamates
1.8.4 Sulphonylurea
1.8.5 Other pesticides
Chapter 2 Synthesis of core-shell magnetic molecular imprinted polymer for the selective determination of imidacloprid in apple samples
2.1 Introduction
2.2 Materials and methods
2.2.1 Reagents and chemicals
2.2.2 Instrumentation
2.2.3 Preparation of imprinted polymers
2.2.3.1 Synthesis of the magnetic nanoparticles
2.2.3.2 Modification of the magnetic nanoparticles
2.2.3.3 Synthesis of the magnetic molecular imprinted polymers
2.2.3.5 Kinetic adsorption procedure
2.2.3.6 Isothermal binding procedure
2.2.3.7 Selectivity of the polymers
2.2.3.8 Reusability
2.2.3.9 Selective extraction of imidacloprid from apple samples
2.3 Results and discussion
2.3.1 Characterization of the imprinted polymers
2.3.1.1 Kinetic adsorption tests
2.3.1.2 Isothermal binding test
2.3.1.3 Selectivity and reusability
2.3.1.4 Applicability of the developed method
Chapter 3 Selective determination of fungicide carbendazim in fruits using β-cyclodextrin based molecularly imprinted polymers
3.1 Introduction
3.2 Materials and methods
3.2.1 Chemicals and reagents
3.2.2 Instrumentation and Chromatographic analysis
3.2.3 Preparation of molecularly imprinted polymers
3.2.4 Kinetic adsorption kinetic test
3.2.5 Adsorption isotherm test
3.2.6 Selectivity binding test
3.2.7 Reusability
3.2.8 Determination of CBZ in fruit samples
3.3 Results and discussion
3.3.1 Synthesis and characterization of the molecularly imprinted polymers
3.3.2 Binding properties of the imprinted polymers
3.3.3 Selective adsorption and reusability
3.3.4 Applicability of the MIPs to real fruit samples
Conclusions
References
Appendices
Acknowledgments
Author resume
本文編號：3790051
【文章頁數(shù)】：130 頁
【學(xué)位級別】：博士
【文章目錄】：
摘要
abstract
Chapter 1 Introduction
1.1 Importance and concept
1.2 Magnetic molecular imprinting polymers
1.3 Mechanism of molecularly imprinted process
1.3.1 Basic approach
1.3.2 Major components of MIPs synthesis
1.3.2.1 Template
1.3.2.2 Functional monomer
1.3.2.3 Cross-linkers
1.3.2.4 Porogenic solvents
1.3.2.5 Initiators
1.4 Basic polymerization approaches
1.5 Challenges and limitations of MIPs application in pesticide detection
1.6 Perceptive Imprinting strategies
1.6.1 Innovative technologies for MIPs
1.6.1.1 Nanoimprinting technology
1.6.1.2 Living/controlled radical polymerization technology
1.6.1.3 Hollow porous polymer synthesis technology
1.6.1.4 Click chemistry cycloaddition reaction technology
1.6.1.5 Microfluidic on-line synthesis technology
1.6.2 Special Imprinting strategies
1.6.2.1 Multi-template imprinting strategy
1.6.2.2 Multi-functional monomer imprinting strategy
1.6.2.3 Dummy imprinting strategy
1.6.2.4 Segment imprinting strategy
1.6.2.5 Composite imprinting material strategy
1.6.3 Stimuli-responsive Imprinting Technologies
1.6.3.1 Photo-responsive technology
1.6.3.2 p H-Responsive technology
1.6.3.3 Dual/multi responsive technology
1.6.3.4 Other responsive technologies
1.7 Physicochemical characterization of MIPs
1.8 Application of MIPs in pesticides residues detection
1.8.1 Organophosphorus pesticides(OPPs)
1.8.2 Triazines
1.8.3 Carbamates
1.8.4 Sulphonylurea
1.8.5 Other pesticides
Chapter 2 Synthesis of core-shell magnetic molecular imprinted polymer for the selective determination of imidacloprid in apple samples
2.1 Introduction
2.2 Materials and methods
2.2.1 Reagents and chemicals
2.2.2 Instrumentation
2.2.3 Preparation of imprinted polymers
2.2.3.1 Synthesis of the magnetic nanoparticles
2.2.3.2 Modification of the magnetic nanoparticles
2.2.3.3 Synthesis of the magnetic molecular imprinted polymers
2.2.3.5 Kinetic adsorption procedure
2.2.3.6 Isothermal binding procedure
2.2.3.7 Selectivity of the polymers
2.2.3.8 Reusability
2.2.3.9 Selective extraction of imidacloprid from apple samples
2.3 Results and discussion
2.3.1 Characterization of the imprinted polymers
2.3.1.1 Kinetic adsorption tests
2.3.1.2 Isothermal binding test
2.3.1.3 Selectivity and reusability
2.3.1.4 Applicability of the developed method
Chapter 3 Selective determination of fungicide carbendazim in fruits using β-cyclodextrin based molecularly imprinted polymers
3.1 Introduction
3.2 Materials and methods
3.2.1 Chemicals and reagents
3.2.2 Instrumentation and Chromatographic analysis
3.2.3 Preparation of molecularly imprinted polymers
3.2.4 Kinetic adsorption kinetic test
3.2.5 Adsorption isotherm test
3.2.6 Selectivity binding test
3.2.7 Reusability
3.2.8 Determination of CBZ in fruit samples
3.3 Results and discussion
3.3.1 Synthesis and characterization of the molecularly imprinted polymers
3.3.2 Binding properties of the imprinted polymers
3.3.3 Selective adsorption and reusability
3.3.4 Applicability of the MIPs to real fruit samples
Conclusions
References
Appendices
Acknowledgments
Author resume
本文編號：3790051
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