肉雞脛骨軟骨發(fā)育不良（TD）是發(fā)生在快速生長(zhǎng)禽類中的一種病因不明的疾病。TD的典型病變可見于脛骨近骺端,其主要特征是無血管化和無鈣化。患TD的家禽表現(xiàn)為身體狀況差,脛骨骨骼變形,行走困難。這種疾病在全世界不僅造成巨大的經(jīng)濟(jì)損失,而且也帶來了嚴(yán)重的動(dòng)物福利問題。中草藥一直被用于治療各種疾病,相比化學(xué)合成藥物,它具有副作用較小、生產(chǎn)成本低廉等特性,適合長(zhǎng)期的服用。1.姜黃素修復(fù)福美雙誘發(fā)肉雞脛骨軟骨發(fā)育不良中的生長(zhǎng)板發(fā)育異常姜黃素是一種鮮黃色的香料,黃素是從姜科、天南星科中的一些植物的根莖中提取的一種姜黃多酚,被稱為姜黃。姜黃素被認(rèn)為可通過抑制破骨細(xì)胞的生成來抑制骨吸收。它是調(diào)控和參與骨重塑的重要分子靶點(diǎn)。然而,目前尚無采用姜黃素治療肉雞TD的研究報(bào)道。本試驗(yàn)旨在研究姜黃素對(duì)TD損傷、禽類的生長(zhǎng)性能、脛骨參數(shù)及對(duì)血管內(nèi)皮生長(zhǎng)因子（VEGF）和Dlx5基因表達(dá)的影響;同時(shí)分析了不同處理?xiàng)l件下肉雞肝臟中的相關(guān)酶類（ALT、ALP、AST）和抗氧化（SOD、MDA、GSH-Px）水平。本試驗(yàn)將400只AA肉雞平均分為4組:對(duì)照組、TD組、TDR（自然恢復(fù)組）和CUR（姜黃素給藥組）。對(duì)照組從第1... 

【文章來源】：華中農(nóng)業(yè)大學(xué)湖北省211工程院校教育部直屬院校

【文章頁數(shù)】：97 頁

【學(xué)位級(jí)別】：博士

【文章目錄】：
摘要
ABSTRACT
ABBREVIATIONS
CHAPTER 1 Introduction
    1.1 Endochondral bone formation
    1.2 Growth plate or epiphyseal plate
    1.3 Avian growth plate
    1.4 Tibial dyschondroplasia (TD)
    1.5 TD and gene expression
        1.5.1 Vascular endothelial growth factor (VEGF)
        1.5.2 Distal-less homeobox 5 (Dlx5)
        1.5.3 Alkaline phosphatase (ALP)
        1.5.4 Peroxisome proliferator-activated receptor γ (PPARγ)
        1.5.5 Type I collagen (Col I)
    1.6 Phytomedicines
        1.6.1 Curcumin
        1.6.2 Emodin
    1.7 Conclusion and hypothesis
CHAPTER 2 Curcumin restored the growth plate (GP) in thiram-induced tibial dyschondroplasia affected chicken
    2.1 Objectives
    2.2 Materials and methods
        2.2.1 Animal welfare and ethics
        2.2.2 Experimental birds
        2.2.3 Experimental design
        2.2.4 Sample collection and handling
        2.2.5 Hematoxylin and eosin (H&E) staining
        2.2.6 Biochemical analysis of serum
        2.2.7 Determination of liver antioxidant enzymes
        2.2.8 Immunohistochemistry (IHC)
        2.2.9 RNA extraction and the synthesis of c DNA
        2.2.10 Reverse transcription q-PCR (RT-q PCR)
        2.2.11 Western blot
        2.2.12 Statistical analysis
    2.3 Results
        2.3.1 Curcumin prevented mortality rate
        2.3.2 Analysis of the performance parameters
        2.3.3 Tibial bone parameters
        2.3.4 Comparison of clinical observations of different groups
        2.3.5 Histological examination
        2.3.6 Effect of curcumin on serum biomarkers
        2.3.7 Effect of curcumin on liver enzymes
        2.3.8 Immunohistochemistry of growth plates
        2.3.9 RT-q PCR and Western blot analysis of VEGF in chicken growth plate
        2.3.10 RT-q PCR and Western blot analysis of Dlx5 in chicken growth plate
    2.4 Discussion
CHAPTER 3 Effect of emodin on ALP, PPARγ and type I collagen genes in thiram-induced tibial dyschondroplasia
    3.1 Objectives
    3.2 Material and methods
    3.3 Results
        3.3.1 Survival rate
        3.3.2 Performance parameters
        3.3.3 Tibial bone parameters
        3.3.4 Emodin improved the clinical outcomes
        3.3.5 Histological examination
        3.3.6 Effect of emodin on serum biomarkers
        3.3.7 Effect of emodin on liver enzymes
        3.3.8 Immunohistochemistry of growth plates
        3.3.9 RT-q PCR and Western blot analysis of ALP in chicken growth plate
        3.3.10 RT-q PCR and Western blot analysis of PPARγ in chicken growth plate
        3.3.11 RT-q PCR of type I collagen in chicken growth plate
    3.4 Discussion
CHAPTER 4 Conclusion and future prospects
    4.1 Study I
    4.2 Study II
    4.3 Future prospects
REFERENCES
LIST OF FIGURES
LIST OF TABLES
ACKNOWLEDGEMENT
APPENDIX I Scientific contribution


【參考文獻(xiàn)】：
期刊論文
[1]Prevention and Treatment of Osteoporosis with Chinese Herbal Medicines[J]. ZHAO Xiao-ling1,2,FENG Yu-xiong1,2,3,PENG Yong1,2 1.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China 2.Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicines,Ministry of Education,Beijing 100193,China 3.College of Basic Medicine,Guangxi University of Chinese Medicine,Nanning 530001,China.  Chinese Herbal Medicines. 2012(04)
[2]Emodin protects rat liver from CCl4-induced fibrogenesis via inhibition of hepatic stellate cells activation[J]. Miao-Xian Dong, Ying-Bo Zhang, Cheng-Chong Li, Yu-Tao Geng, Li Zhou, Xue-Yan Li, Ji-Cheng Liu, Ying-Cai Niu, The Institute of Medicine, Qiqihar Medical University, Qiqihar 161042, Heilongjiang Province, China Yan Jia, Department of Pathophysiology, Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang Province, China.  World Journal of Gastroenterology. 2009(38)



本文編號(hào)：3633591