脛骨軟骨發(fā)育不良（TD）是一種影響快速生長期肉雞的骨骼異常發(fā)育疾病。除了對全世界的禽業(yè)造成巨大的經(jīng)濟損失外,TD也引起了對動物福利的關(guān)注。TD的特點是在脛骨的近端生長板處存在無血管化,無礦化的白色不透明軟骨栓�；糡D的雞表現(xiàn)出不良的身體狀況,畸形的脛骨以及跛行。1.蛇床子素:一種香豆素衍生物通過調(diào)節(jié)雞的BMP-2和RUNX-2表達(dá)緩解福美雙所致的脛骨軟骨發(fā)育不良禽脛骨軟骨發(fā)育不良發(fā)生于快速生長的肉雞,約占肉雞腿病的30%。本研究旨在評估蛇床子素對禽類脛骨軟骨發(fā)育不良（TD）的療效。將240只雞平均分為對照組,TD組和蛇床子素治療（osthole）組（n=80）。TD和osthole組的雞在4–7天以添加50mg/kg飼料重的四甲基秋蘭姆二硫（thiram）的飼料進行飼喂,然后在8–18天通過口服的方式給予雞20 mg/kg體重的蛇床子素。飼喂Thiram會導(dǎo)致跛行,增加死亡率,并降低生產(chǎn)參數(shù),堿性磷酸酶（ALP）,超氧化物歧化酶（SOD）,總抗氧化能力（TAOC）,谷胱甘肽過氧化物酶（GSH-Px）,天冬氨酸氨基轉(zhuǎn)移酶（AST）,丙氨酸氨基轉(zhuǎn)移酶（ALT）,丙二醛（MDA）的水平和并使... 

【文章來源】：華中農(nóng)業(yè)大學(xué)湖北省 211工程院校 教育部直屬院校

【文章頁數(shù)】：87 頁

【學(xué)位級別】：博士

【文章目錄】：
摘要
ABSTRACT
CHAPTER 1 General Introduction
    1.1.Endochondral bone formation and ossification
    1.2.Epiphyseal growth plate
        1.2.1.Reserve zone
        1.2.2.Proliferative zone
        1.2.3.Transition zone
        1.2.4.Prehypertrophic zone
        1.2.5.Hypertrophic zone
    1.3.Avian growth plate
    1.4.Tibial dyschondroplasia(TD)
    1.5.Tetramethylthiuram disulfide(Thiram)
    1.6.TD and gene expression
        1.6.1.Role of Bone morphogenetic protein-2(BMP-2)in TD
        1.6.2.Role of Runt-related transcription factor-2(RUNX-2)in TD
        1.6.3.Role of Hypoxia Inducible Factor-1α (HIF-1α)in TD
        1.6.4.Role of Tissue inhibitor of metalloproteinase-3(TIMP-3)in TD
        1.6.5.Role of B-cell lymphoma-2(BCL-2)in TD
    1.7.Prevention and Treatment of TD
    1.8.Traditional Chinese Medicines
        1.8.1.Osthole
        1.8.2.Puerarin
    1.9.Conclusion and Hypothesis
CHAPTER 2 Osthole:A Coumarin Derivative Assuage Thiram-Induced Tibial Dyschondroplasia by Regulating BMP-2 and RUNX-2 Expressions in Chickens
    2.1.Introduction
    2.2.Objectives
    2.3.Materials and methods
        2.3.1.Animal ethics
        2.3.2.Experimental Birds,Chemical Reagents,and Medicine
        2.3.3.Experiment Design
        2.3.4.Samples Collection
        2.3.5.Mortality Assay,Production Parameters,and Visceral Organs Indices
        2.3.6.Biochemical and Antioxidants Analyses
        2.3.7.Tibia Bone Parameters
        2.3.8.Hematoxylin and Eosin(H&E)Staining and Immunohistochemistry
        2.3.9.Reverse Transcription Quantitative Polymerase Chain Reaction(RT-q PCR)
        2.3.10.Western Blotting
        2.3.11.Statistical Analyses
    2.4.Results
        2.4.1.Clinical Observations of Thiram–Induced TD
        2.4.2.Chicken Mortality and Survival Rate
        2.4.3.Production Parameters Analysis
        2.4.4.Tibia Parameters Analysis
        2.4.5.Serum Biochemical and Liver Antioxidants Analyses
        2.4.6.Visceral Organs Indices
        2.4.7.Histological Examination of the Tibial Growth Plates
        2.4.8.Immunohistochemical Analysis
        2.4.9.The m RNA and Protein Expressions of BMP-2 and RUNX-2
    2.5.Discussion
CHAPTER 3 Puerarin enhance vascular proliferation and halt apoptosis in thiram-induced avian tibial dyschondroplasia by regulating HIF-1α,TIMP-3 and BCL-2 expressions
    3.1.Introduction
    3.2.Objectives
    3.3.Material and methods
        3.3.1.Ethical approval,TD induction and puerarin administration
        3.3.2.Samples collection
        3.3.3.Percent survival,production and visceral organ’parameters
        3.3.4.Serum biochemical and antioxidant analyses
        3.3.5.Tibia bone morphometry
        3.3.6.Hematoxylin& Eosin(H&E)staining and immunohistochemistry
        3.3.7.Reverse transcription quantitative polymerase chain reaction(RT-q PCR)
        3.3.8.Western blot
        3.3.9.Statistical analyses
    3.4.Results
        3.4.1.Clinical signs,percent survival and mortality
        3.4.2.Puerarin enhance chicken production parameters
        3.4.3.Puerarin assuage hepatic dysfunction and oxidative stress
        3.4.4.Puerarin impact on visceral organ weight and indices
        3.4.5.Tibia bone morphometry and growth plate morphology
        3.4.6.Histopathology of the tibial growth plate
        3.4.7.Immunohistochemical analysis
        3.4.8.Expression of HIF-1α,TIMP-3 and BCL-2
    3.5.Discussion
CHAPTER 4 Conclusion and future prospects
    4.1.Study I
    4.2.Study II
    4.3.Future prospects
REFERENCES
ACKNOWLEDGEMENT
SCIENTIFIC CONTRIBUTION



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