葡萄籽提取物及龜板提取物對脛骨軟骨發(fā)育不良肉雞治療效果的研究
發(fā)布時間：2021-09-02 00:47
脛骨軟骨發(fā)育不良（TD）屬于禽類代謝紊亂病,主要發(fā)生在快速生長的禽類中,特別是在肉雞中。其特征是生長板（GP）的非礦化和非血管化,導(dǎo)致肉雞跛行及軟骨細(xì)胞的異常生長。據(jù)報道,TD的發(fā)病率約占家禽骨病的30%,其在中國發(fā)病率約為10%,并能導(dǎo)致農(nóng)民的利潤率嚴(yán)重下降。TD的臨床和亞臨床癥狀主要為免疫力降低、骨髓炎、生長緩慢、及乳腺囊腫形成。進(jìn)而引起動物倫理和福利問題,降低肉雞的胴體和肉的質(zhì)量。1.葡萄籽提取物通過下調(diào)CA2基因和增強(qiáng)肝功能恢復(fù)受脛骨軟骨發(fā)育不良影響的雞脛骨軟骨發(fā)育不良（TD）是一種嚴(yán)重的肉雞代謝綜合征,其表現(xiàn)為生長板（GP）向脛骨干骺端增生。葡萄籽提取物（GSE）是是一種天然的植物提取物,在抗氧化,抗炎、抗癌和減少機(jī)體氧化損傷方面起著重要作用。本實驗旨在評估GSE對發(fā)生TD的肉雞的治療作用。為此,我們購買了210只雛雞并在前3天喂食正常的初始飼料。之后,所有雛雞分成兩組,對照組為70只雞（正常飲食）,另一組為試驗組140雞（正常飲食+福美雙50mg/kg）。試驗組在第7天又被平分為2組,分別為TD組和GSE組。飼喂結(jié)果顯示:與TD組相比,GSE顯著（P<0.05）減少了...
【文章來源】：華中農(nóng)業(yè)大學(xué)湖北省 211工程院校 教育部直屬院校
【文章頁數(shù)】：94 頁
【學(xué)位級別】：博士
【文章目錄】：
摘要
ABSTRACT
List of abbreviations
CHAPTER1 Introduction
1.1 Endochondral ossification
1.2 Bone developmental process
1.3 Growth plate structure
1.4 Avian epiphyseal plate
1.5 Tibial dyschondroplasia(TD)
1.6 TD and gene expression
1.6.1 Carbonic Anhydrase2 (CA2)
1.6.2 Hypoxia Inducible Factor1 (HIF-1α)and Hsp90
1.6.3 AKT and PI3K
1.7 Grape Seed Extract
1.8 Plastrum Testudinis Extract
1.9 Inference and hypothesis
CHAPTER2 Recovery of Chickens Affected with Tibial Dyschondroplasia by Application of Grape Seed Extract Through Downregulating CA2 Gene and Enhancing Liver Functions
2.1 Introduction and objectives of the study
2.2 Materials and Methods
2.2.1 Ethical Approval
2.2.2 Experimental Design
2.2.3 Analysis of Production Performance
2.2.4 Analysis of Blood Biochemistry
2.2.5 Analysis of Liver Antioxidants and Hematoxylin& Eosin(H&E)Staining
2.2.6 Extraction of RNA and RT-q PCR Analysis
2.2.7 Western blotting analysis
2.2.8 Immunohistochemistry
2.2.9 Statistical analysis
2.3 Results
2.3.1 Growth performance and mortality
2.3.2 Effect of GSE on TD score and growth-plate width
2.3.3 Analysis of blood biochemistry and liver histopathological inspection
2.3.4 Evaluation of liver antioxidant status
2.3.5 CA2 m RNA and protein expression in tibia growth plate
2.3.6 Immunohistochemistry of growth plates
2.4 DISCUSSION
CHAPTER3 Effect of Grape Seed Extract on Tibial Dyschondroplasia Incidence,Liver Weight,and Tibial Angiogenesis in Chickens
3.1 Introduction and objectives of the study
3.2 Materials and methods
3.2.1 Animal welfare and ethics
3.2.2 Birds raising plan
3.2.3 Morphological inspection,tibia parameters and liver weight
3.2.4 Hematoxylin& Eosin(H&E)staining and Immunohistochemistry(IHC)
3.2.5 Real time quantitative PCR(RT-q PCR)
3.2.6 Western Blot analysis
3.2.7 Statistical analysis
3.3 RESULTS
3.3.1 Weight gain and mortality
3.3.2 Effect of GSE on TD incidence and liver weight
3.3.3 The general tibial parameters examination
3.3.4 Clinical evaluation of the TD and GSE chickens
3.3.5 Histopathological investigation of the growth plates of tibia
3.3.6 Immunohistochemistry of Tibial growth plates
3.3.7 The HIF-1αand Hsp90 m RNA and protein expression in growth plate
3.4 DISCUSSION
CHAPTER4 Plastrum Testudinis Extract Mitigates Thiram Toxicity in Broilers via Regulating PI3K/AKT Signaling
4.1 Introduction and objectives of the study
4.2 Materials and methods
4.2.1 Reagents and compounds
4.2.2 Approval of Ethics
4.2.3 Experimental plan
4.2.4 Analyses of Production Parameters and Tibia Parameters
4.2.5 Analysis of Blood Biochemistry
4.2.6 Liver weight and Antioxidants Analysis
4.2.7 Hematoxylin& Eosin(H&E)staining and Immunohistochemistry
4.2.8 RNA Extraction and RT-q PCR
4.2.9 Western Blot analysis
4.2.10 Statistical analysis
4.3 RESULTS
4.3.1 Production parameters
4.3.2 Effect of PTE on growth-plate size,tibia parameters,and TD score
4.3.3 Biochemical criterion analysis of serum and liver histopathology
4.3.4 Liver weight and determination of antioxidant status in liver
4.3.5 Histopathological investigation of the growth plates of tibia
4.3.6 Immunohistochemistry of Tibial growth plates
4.3.7 AKT and PI3K genes and protein expression in growth plate
4.4 DISCUSSION
CHAPTER5 Conclusions and Future Prospects
5.4 Future Prospects
References
Acknowledgement
本文編號：3377974
【文章來源】：華中農(nóng)業(yè)大學(xué)湖北省 211工程院校 教育部直屬院校
【文章頁數(shù)】：94 頁
【學(xué)位級別】：博士
【文章目錄】：
摘要
ABSTRACT
List of abbreviations
CHAPTER1 Introduction
1.1 Endochondral ossification
1.2 Bone developmental process
1.3 Growth plate structure
1.4 Avian epiphyseal plate
1.5 Tibial dyschondroplasia(TD)
1.6 TD and gene expression
1.6.1 Carbonic Anhydrase2 (CA2)
1.6.2 Hypoxia Inducible Factor1 (HIF-1α)and Hsp90
1.6.3 AKT and PI3K
1.7 Grape Seed Extract
1.8 Plastrum Testudinis Extract
1.9 Inference and hypothesis
CHAPTER2 Recovery of Chickens Affected with Tibial Dyschondroplasia by Application of Grape Seed Extract Through Downregulating CA2 Gene and Enhancing Liver Functions
2.1 Introduction and objectives of the study
2.2 Materials and Methods
2.2.1 Ethical Approval
2.2.2 Experimental Design
2.2.3 Analysis of Production Performance
2.2.4 Analysis of Blood Biochemistry
2.2.5 Analysis of Liver Antioxidants and Hematoxylin& Eosin(H&E)Staining
2.2.6 Extraction of RNA and RT-q PCR Analysis
2.2.7 Western blotting analysis
2.2.8 Immunohistochemistry
2.2.9 Statistical analysis
2.3 Results
2.3.1 Growth performance and mortality
2.3.2 Effect of GSE on TD score and growth-plate width
2.3.3 Analysis of blood biochemistry and liver histopathological inspection
2.3.4 Evaluation of liver antioxidant status
2.3.5 CA2 m RNA and protein expression in tibia growth plate
2.3.6 Immunohistochemistry of growth plates
2.4 DISCUSSION
CHAPTER3 Effect of Grape Seed Extract on Tibial Dyschondroplasia Incidence,Liver Weight,and Tibial Angiogenesis in Chickens
3.1 Introduction and objectives of the study
3.2 Materials and methods
3.2.1 Animal welfare and ethics
3.2.2 Birds raising plan
3.2.3 Morphological inspection,tibia parameters and liver weight
3.2.4 Hematoxylin& Eosin(H&E)staining and Immunohistochemistry(IHC)
3.2.5 Real time quantitative PCR(RT-q PCR)
3.2.6 Western Blot analysis
3.2.7 Statistical analysis
3.3 RESULTS
3.3.1 Weight gain and mortality
3.3.2 Effect of GSE on TD incidence and liver weight
3.3.3 The general tibial parameters examination
3.3.4 Clinical evaluation of the TD and GSE chickens
3.3.5 Histopathological investigation of the growth plates of tibia
3.3.6 Immunohistochemistry of Tibial growth plates
3.3.7 The HIF-1αand Hsp90 m RNA and protein expression in growth plate
3.4 DISCUSSION
CHAPTER4 Plastrum Testudinis Extract Mitigates Thiram Toxicity in Broilers via Regulating PI3K/AKT Signaling
4.1 Introduction and objectives of the study
4.2 Materials and methods
4.2.1 Reagents and compounds
4.2.2 Approval of Ethics
4.2.3 Experimental plan
4.2.4 Analyses of Production Parameters and Tibia Parameters
4.2.5 Analysis of Blood Biochemistry
4.2.6 Liver weight and Antioxidants Analysis
4.2.7 Hematoxylin& Eosin(H&E)staining and Immunohistochemistry
4.2.8 RNA Extraction and RT-q PCR
4.2.9 Western Blot analysis
4.2.10 Statistical analysis
4.3 RESULTS
4.3.1 Production parameters
4.3.2 Effect of PTE on growth-plate size,tibia parameters,and TD score
4.3.3 Biochemical criterion analysis of serum and liver histopathology
4.3.4 Liver weight and determination of antioxidant status in liver
4.3.5 Histopathological investigation of the growth plates of tibia
4.3.6 Immunohistochemistry of Tibial growth plates
4.3.7 AKT and PI3K genes and protein expression in growth plate
4.4 DISCUSSION
CHAPTER5 Conclusions and Future Prospects
5.4 Future Prospects
References
Acknowledgement
本文編號：3377974
本文鏈接：http://sikaile.net/shoufeilunwen/nykjbs/3377974.html
最近更新
教材專著
