本文選題：豬 + 卵母細胞��； 參考：《中國農(nóng)業(yè)科學院》2016年博士后論文

【摘要】：豬是廣泛飼養(yǎng)的經(jīng)濟動物,提供重要的肉食品來源；也是重要的實驗動物,是人類醫(yī)學的試驗模型之一。microRNAs (miRNAs)參與如細胞增殖、分化等許多生物學過程的調(diào)控。已有的研究發(fā)現(xiàn),在許多物種的卵母細胞成熟和早期胚胎發(fā)育過程中,miRNA的表達豐度都是呈現(xiàn)動態(tài)變化的。miRNA可影響卵母細胞成熟、早期胚胎卵裂、母源mRNA降解等過程。但是,目前對于豬卵母細胞成熟和早期胚胎發(fā)育過程中miRNA的研究還相對較少。本研究選取GV期卵母細胞、MII期卵母細胞、4-細胞胚胎和囊胚四個時間點的樣本,采用solexa測序的方法對miRNA的表達譜進行系統(tǒng)分析,篩選出了在豬卵母細胞成熟及早期胚胎發(fā)育過程中有重要作用的miRNAs,在對差異表達的miRNAs的靶基因進行預測后,還對這些預測的靶基因進行通路分析。此外,通過Q-PCR驗證solexa測序結(jié)果的同時,對關鍵]miRNA如mir-27、let-7i等進行進一步試驗研究,為闡明豬卵母細胞成熟及早期胚胎發(fā)育過程中miRNA的調(diào)控機制奠定了基礎。
[Abstract]:Pigs are widely raised economic animals and provide important sources of meat food. They are also important experimental animals. MicroRNAs (miRNAs) are involved in many biological processes such as cell proliferation and differentiation. It has been found that the abundance of miRNA expression in oocyte maturation and early embryonic development of many species is a dynamic change. MiRNA may affect oocyte maturation, early embryo cleavage, and the degradation of mother mRNA. However, there are few studies on miRNA in pig oocytes maturation and early embryonic development. In this study, the expression profiles of miRNAs were systematically analyzed by solexa sequencing from four time points of GV phase oocyte phase II oocytes, embryos and blastocysts. MiRNAss, which play an important role in the maturation and early embryonic development of porcine oocytes, were screened. After predicting the target genes of differentially expressed miRNAs, the pathway of these predicted genes was analyzed. In addition, the solexa sequencing results were verified by Q-PCR, and the key miRNAs, such as mir-27let-7i, were further studied, which laid a foundation for elucidating the regulation mechanism of miRNAs during maturation and early embryonic development of porcine oocytes.
【學位授予單位】：中國農(nóng)業(yè)科學院
【學位級別】：博士后
【學位授予年份】：2016
【分類號】：S828

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本文編號：2070722