天堂国产午夜亚洲专区-少妇人妻综合久久蜜臀-国产成人户外露出视频在线-国产91传媒一区二区三区

基于transformer2和doublesex級聯(lián)互作的煙粉虱性別決定機制研究

發(fā)布時間:2018-05-07 17:15

  本文選題:煙粉虱 + 性別決定; 參考:《中國農(nóng)業(yè)科學院》2016年博士論文


【摘要】:性別決定是生物兩性形成和正常生長發(fā)育的基礎,對種群延續(xù)意義重大。煙粉虱是一種世界性農(nóng)業(yè)害蟲,為單雙倍體昆蟲。雄蟲來自未受精的卵為單倍體,雌蟲來自受精卵為雙倍體。為了研究煙粉虱的性別決定機制,對其性別決定中的兩個關(guān)鍵基因transformer2(Bttra2)和doublesex(Btdsx)進行了克隆和分析,并進一步利用RNAi技術(shù)闡述了其在煙粉虱性別決定中的作用。研究結(jié)果表明煙粉虱Bttra2全長1702bp,由10個外顯子和9個內(nèi)含子組成;Btdsx全長2339bp,由7個外顯子和6個內(nèi)含子組成。發(fā)現(xiàn)Bttra2有四種剪接形式,編碼四種蛋白,表達豐度雄蟲高于雌蟲;而Btdsx有13種剪接形式,編碼六種蛋白,表達豐度雄蟲也高于雌蟲。明確了transformer2和Btdsx在煙粉虱性別決定通路中均發(fā)揮至關(guān)重要作用且兩者相互影響和制約,Bttra2/Btdsx基因的干擾影響Vg基因和Btdsx/Bttra2的表達量,造成煙粉虱雄蟲生殖器官尾針畸形。研究結(jié)果對于闡明煙粉虱性別決定機制具有重要理論意義。
[Abstract]:Sex determination is the basis for the formation of biological hermaphroditism and normal growth and development, which is of great significance for population continuation. Bemisia Tabaci is a worldwide agricultural pest, a haploidy insect. Males from unfertilized eggs were haploid and females from fertilized eggs were diploidy. In order to study the sex determination mechanism of Bemisia Tabaci, two key genes, transformer2Bttra2) and DoublesexexBtdsx), were cloned and analyzed, and their roles in sex determination of Bemisia Tabaci were further elucidated by using RNAi technique. The results showed that the total length of Bemisia Tabaci Bttra2 was 1702 BP, which consisted of 10 exons and 9 introns, which consisted of 7 exons and 6 introns. It was found that Bttra2 had four splicing forms, encoding four proteins, and that Btdsx had 13 splicing forms, encoding six proteins, and expressed more abundant male than female. It is clear that transformer2 and Btdsx play an important role in the sex-determining pathway of Bemisia Tabaci and that the interference of Bttra2 / Btdsx gene affects the expression of Vg gene and Btdsx/Bttra2 and causes malformation of male reproductive organ of Bemisia Tabaci. The results are of great theoretical significance to clarify the sex determination mechanism of Bemisia Tabaci (Bemisia Tabaci).
【學位授予單位】:中國農(nóng)業(yè)科學院
【學位級別】:博士
【學位授予年份】:2016
【分類號】:S433

【相似文獻】

相關(guān)博士學位論文 前1條

1 郭利桃;基于transformer2和doublesex級聯(lián)互作的煙粉虱性別決定機制研究[D];中國農(nóng)業(yè)科學院;2016年

,

本文編號:1857775

資料下載
論文發(fā)表

本文鏈接:http://sikaile.net/shoufeilunwen/nykjbs/1857775.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶42b46***提供,本站僅收錄摘要或目錄,作者需要刪除請E-mail郵箱bigeng88@qq.com
国产传媒一区二区三区| 精品人妻一区二区三区免费| 欧美极品欧美精品欧美| 国产不卡的视频在线观看| 欧美日韩校园春色激情偷拍 | 午夜福利黄片免费观看| 欧美一区二区三区高潮菊竹| 国产一区二区不卡在线播放| 午夜精品成年人免费视频| 夫妻性生活一级黄色录像| 欧美日韩精品久久第一页| 国产一区一一一区麻豆| 久久偷拍视频免费观看| 成人免费观看视频免费| 欧美不卡一区二区在线视频| 欧美日韩国产综合在线| 九九蜜桃视频香蕉视频| 国产视频福利一区二区| 日韩亚洲精品国产第二页| 色哟哟在线免费一区二区三区| 插进她的身体里在线观看骚| 麻豆精品视频一二三区| 亚洲熟女诱惑一区二区| 亚洲中文字幕在线视频频道 | 欧洲日韩精品一区二区三区| 性感少妇无套内射在线视频| 91麻豆视频国产一区二区| 日韩午夜福利高清在线观看| 亚洲第一香蕉视频在线| 天海翼高清二区三区在线| 国产日韩欧美综合视频| 我的性感妹妹在线观看| 99久久国产亚洲综合精品| 婷婷基地五月激情五月| 99国产一区在线播放| 欧美黑人黄色一区二区| 国产精品乱子伦一区二区三区| 青青久久亚洲婷婷中文网| 国产又黄又爽又粗视频在线| 免费在线观看激情小视频| 中文字幕日韩无套内射|