【摘要】：細(xì)胞的分裂和分化受到多種植物激素的調(diào)控。脫落酸(ABA)和生長(zhǎng)素參與根生長(zhǎng)和發(fā)育的調(diào)控,然而對(duì)其分子機(jī)制我們還知之甚少。之前的研究表明生長(zhǎng)素響應(yīng)因子2(ARF2)是ABA調(diào)控種子萌發(fā)和初生根生長(zhǎng)通路中的負(fù)調(diào)控因子。PLT蛋白在控制根分生組織活性方面發(fā)揮重要作用。在本研究中,我們發(fā)現(xiàn)arf2突變體根生長(zhǎng)對(duì)ABA超敏感的表型是由于ABA對(duì)靜止中心細(xì)胞(QC)分裂的抑制以及對(duì)根尖分生區(qū)(RAM)細(xì)胞分裂和分化的抑制引起的。ABA通過(guò)ARF2降低PIN1, PIN3和PIN7在中柱的表達(dá)以及降低生長(zhǎng)素在根尖分生區(qū)的積累。然而,在arf2突變體中,QC和柱細(xì)胞中高濃度的生長(zhǎng)素抑制其分裂,而根尖分生區(qū)中低濃度的生長(zhǎng)素抑制分生區(qū)細(xì)胞的分裂和伸長(zhǎng)。與野生型相比,ABA處理減少了PIN1, PIN3, PIN7的表達(dá),卻增加了PIN4在arf2-101根尖中的積累。PIN4的突變可以部分逆轉(zhuǎn)ABA對(duì)口arf2-101根生長(zhǎng)的抑制。此外,在根尖分生區(qū),ARF2在轉(zhuǎn)錄水平上正調(diào)控ABA所介導(dǎo)的PLT1的表達(dá),但可能在轉(zhuǎn)錄后水平上間接調(diào)控PLT2。與arf2-101、pltl-4、plt2-2和plt1-4plt2-2突變體相比,arf2-101plt1-4雙突對(duì)ABA更為敏感,分生區(qū)細(xì)胞數(shù)目更少。然而,利用DEX誘導(dǎo)型的轉(zhuǎn)基因株系Pro35S:PLT2-GR過(guò)表達(dá)PLT2,我們發(fā)現(xiàn)PLT2在很大程度上促進(jìn)細(xì)胞分裂,并且完全阻斷arf2-101根分生組織的細(xì)胞分化,而這一過(guò)程可以被外源施加的ABA部分抑制。這表明ABA可通過(guò)ARF2依賴和不依賴的方式調(diào)控根的分生區(qū)。我們的結(jié)果揭示了擬南芥中ARF2與PLTs及PINs蛋白協(xié)同作用ABA介導(dǎo)的根分生組織調(diào)控的復(fù)雜網(wǎng)絡(luò)。
[Abstract]:Cell division and differentiation are regulated by many plant hormones. Abscisic acid (ABA) and auxin are involved in the regulation of root growth and development, but little is known about its molecular mechanism. Previous studies have shown that auxin response factor 2 (ARF2) is a negative regulatory factor in seed germination and primary rooting growth pathway regulated by ABA. PLT protein plays an important role in controlling the activity of root meristem. In this study, we found that the phenotype of arf2 mutant root growth to ABA hypersensitivity was due to the inhibition of (QC) division by ABA and the inhibition of (RAM) cell division and differentiation in apical meristem region. ABA reduced PIN1, through ARF2. The expression of PIN3 and PIN7 in the middle column and the decrease of auxin accumulation in the apical meristem. However, in arf2 mutants, high concentrations of auxin in QC and columnar cells inhibited its division, while low concentrations of auxin in the apical meristem inhibited the division and elongation of meristem cells. Compared with wild type, ABA treatment decreased the expression of PIN1, PIN3, PIN7, but increased the accumulation of PIN4 in arf2-101 root tip. The mutation of PIN4 partially reversed the inhibition of ABA on arf2-101 root growth. In addition, at the apical meristem region, ARF2 is regulating the expression of PLT1 mediated by ABA at the transcriptional level, but may indirectly regulate PLT2. at the posttranscriptional level. Compared with arf2-101,pltl-4,plt2-2 and plt1-4plt2-2 mutants, arf2-101plt1-4 double processes are more sensitive to ABA and have less number of meristematic cells. However, using DEX inducible transgenic strain Pro35S:PLT2-GR to overexpression PLT2, we found that PLT2 promoted cell division to a great extent and completely blocked the differentiation of arf2-101 root meristem. This process can be partially inhibited by exogenous ABA. This indicates that ABA can regulate the meristematic region of root by ARF2 dependent and independent. Our results reveal a complex network of ABA-mediated root meristem regulation mediated by ARF2, PLTs and PINs proteins in Arabidopsis thaliana.
【學(xué)位授予單位】：中國(guó)農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2016
【分類號(hào)】：Q943.2

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