甲胺磷在犬體內(nèi)的死后分布
發(fā)布時間：2019-06-17 12:01
【摘要】：目的建立甲胺磷的犬灌胃染毒致死模型,觀察甲胺磷在犬體內(nèi)的死后分布規(guī)律。方法犬經(jīng)8倍LD50(7.4mg/kg)劑量甲胺磷灌胃后,觀察其中毒癥狀,死亡后即刻解剖,分別取心、肝、脾、肺、腎、腦、右上肢肌、右下肢肌、胸肌、胃組織、心血、膽汁、玻璃體液和尿液,GC/MS和GC法檢測其中甲胺磷含量。結(jié)果犬8倍LD50甲胺磷灌胃染毒后20min內(nèi)出現(xiàn)中毒癥狀(,53.3±14.1)min死亡。各組織臟器及體液中甲胺磷含量由高到低分別為胃(99.84±0.87)μg/g、脾(46.87±28.67)μg/g、肝(43.82±22.74)μg/g、腎(43.79±29.04)μg/g、心血(35.36±13.98)μg/mL、肺(35.25±18.59)μg/g、尿34.81μg/mL、胸肌(19.23±17.18)μg/g、右上肢(16.92±8.98)μg/g、心(15.09±6.11)μg/g、右下肢(12.83±7.63)μg/g、腦(10.91±4.13)μg/g、膽汁(6.75±1.45)μg/mL、玻璃體液(6.22±4.97)μg/mL。結(jié)論甲胺磷在犬體內(nèi)死后分布不均,胃、脾、肝、腎、心血、肺、尿檢材中含量較高,可作為疑似甲胺磷中毒毒物分析的檢材。
[Abstract]:Objective to establish a lethal model of methamidophosphate in dogs exposed to intragastric administration and to observe the postmortem distribution of methamidophosphate in dogs. Methods the poisoning symptoms of dogs were observed and dissected immediately after death. The contents of methamidophosphate were measured by GC/MS and GC, respectively. The heart, liver, spleen, lung, kidney, brain, right upper limb muscle, right lower extremity muscle, chest muscle, gastric tissue, cardiac blood, bile, vitreous body fluid and urine were detected by GC/MS and GC. Results the poisoning symptoms in 20min were (53.3 鹵14.1) min death in dogs exposed to 8 times LD50 methamidophosphate intragastrically. The contents of methamidophosphate in organs and body fluids were (99.84 鹵0.87) 渭 g / g in stomach, (46.87 鹵28.67) 渭 g / g in spleen, (43.82 鹵22.74) 渭 g / g in liver, (43.79 鹵29.04) 渭 g / g in kidney, (35.36 鹵13.98) 渭 g / ml in heart blood, (35.25 鹵18.59) 渭 g / g in lung, 34.81 渭 g / mL, 渭 g / g in upper right side, respectively. The heart was (15.09 鹵6.11) 渭 g 路g / g, the right lower limb (12.83 鹵7.63) 渭 g 路g / g, the brain (10.91 鹵4.13) 渭 g / g, the bile (6.75 鹵1.45) 渭 g / mL, the vitreous body fluid (6.22 鹵4.97) 渭 g 路L ~ (- 1). Conclusion methamidophos is unevenly distributed in dogs after death, and the contents of methamidophosphate in stomach, spleen, liver, kidney, heart blood, lung and urine are high, which can be used as the detection material for the analysis of suspected methamidophosphate poisoning.
【作者單位】： 山西醫(yī)科大學法醫(yī)學院;
【基金】：“十一五”國家科技支撐計劃項目(2007BAK26B05) 山西省高等學校中青年拔尖創(chuàng)新人才支持計劃資助(2008)
【分類號】：D919
[Abstract]:Objective to establish a lethal model of methamidophosphate in dogs exposed to intragastric administration and to observe the postmortem distribution of methamidophosphate in dogs. Methods the poisoning symptoms of dogs were observed and dissected immediately after death. The contents of methamidophosphate were measured by GC/MS and GC, respectively. The heart, liver, spleen, lung, kidney, brain, right upper limb muscle, right lower extremity muscle, chest muscle, gastric tissue, cardiac blood, bile, vitreous body fluid and urine were detected by GC/MS and GC. Results the poisoning symptoms in 20min were (53.3 鹵14.1) min death in dogs exposed to 8 times LD50 methamidophosphate intragastrically. The contents of methamidophosphate in organs and body fluids were (99.84 鹵0.87) 渭 g / g in stomach, (46.87 鹵28.67) 渭 g / g in spleen, (43.82 鹵22.74) 渭 g / g in liver, (43.79 鹵29.04) 渭 g / g in kidney, (35.36 鹵13.98) 渭 g / ml in heart blood, (35.25 鹵18.59) 渭 g / g in lung, 34.81 渭 g / mL, 渭 g / g in upper right side, respectively. The heart was (15.09 鹵6.11) 渭 g 路g / g, the right lower limb (12.83 鹵7.63) 渭 g 路g / g, the brain (10.91 鹵4.13) 渭 g / g, the bile (6.75 鹵1.45) 渭 g / mL, the vitreous body fluid (6.22 鹵4.97) 渭 g 路L ~ (- 1). Conclusion methamidophos is unevenly distributed in dogs after death, and the contents of methamidophosphate in stomach, spleen, liver, kidney, heart blood, lung and urine are high, which can be used as the detection material for the analysis of suspected methamidophosphate poisoning.
【作者單位】： 山西醫(yī)科大學法醫(yī)學院;
【基金】：“十一五”國家科技支撐計劃項目(2007BAK26B05) 山西省高等學校中青年拔尖創(chuàng)新人才支持計劃資助(2008)
【分類號】：D919
【參考文獻】
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