【摘要】： STR是一類具有長度多態(tài)性的DNA序列,按照孟德爾共顯性方式遺傳,故已被作為遺傳標(biāo)記廣泛應(yīng)用在生物科學(xué)和醫(yī)學(xué)等領(lǐng)域的研究與實(shí)踐當(dāng)中。STR的應(yīng)用非常廣泛,特別是STR基因座的PCR擴(kuò)增片段長度多態(tài)性檢測技術(shù),該技術(shù)有著十分顯著的適用于法醫(yī)學(xué)個(gè)人識別的特點(diǎn)。本研究選擇了D1S549,D3S1744, D6S366,D5S818、D8S1179、D13S317、D16S539等7個(gè)STR基因座,采用PCR、DNA聚丙烯酰胺凝膠電泳銀染分型技術(shù)對長春漢族群體這7個(gè)位點(diǎn)的基因頻率分布進(jìn)行了調(diào)查,得出此7個(gè)基因座在長春漢族人群中基因型頻率分布符合Hardy-Weinberg平衡,提供的信息量大,有較高的個(gè)人識別能力和非父排除概率。除D6S366位點(diǎn)雜合度為0.6333,小于0.8,其余幾個(gè)基因座的雜合度均在0.8以上而多態(tài)信息含量均在0.7以上,屬多態(tài)信息含量較高的基因座,個(gè)人識別能力除D6S366略低(0.7793),其余都在0.9以上,非父排除概率除D3S1744和D6S366略低(分別為0.4184和0.3617),其余均在0.6以上,屬實(shí)用價(jià)值比較高的基因座。
[Abstract]:STR is a kind of DNA sequence with length polymorphism, which is inherited according to Mendelian codominant mode, so it has been widely used as genetic marker in the research and practice of bioscience and medicine. In particular, the PCR amplified fragment length polymorphism detection technique of STR loci is very suitable for forensic personal recognition. Seven STR loci D1S549, D3S1744, D6S366, D5S818, D8S1179, D13S317, D16S539 were selected. PCR,DNA polyacrylamide gel electrophoresis silver staining technique was used to investigate the gene frequency distribution of these seven loci in Changchun Han population. It is concluded that the genotypic frequency distribution of these seven loci in Changchun Han population accords with Hardy-Weinberg balance, provides a large amount of information, and has high personal recognition ability and non-paternity exclusion probability. Except that the heterozygosity of D6S366 locus is 0.6333, which is less than 0.8, the heterozygosity of the other loci is above 0.8, and the polymorphism information content is above 0.7. it belongs to the loci with high polymorphism information content. The personal recognition ability was slightly lower (0.7793) than that of D6S366, and the probability of non-paternal exclusion was slightly lower than that of D3S1744 and D6S366 (0.4184 and 0.3617, respectively), which belonged to loci with high practical value.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：D919

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王冰梅,莊文越,項(xiàng)小豐,曲萌,李正yN,莊文卓;吉林地區(qū)漢族人群12個(gè)STR基因座的遺傳多態(tài)性[J];第四軍醫(yī)大學(xué)學(xué)報(bào);2005年22期

2 辛軍平,陳國弟,張紅玲;D1S549、D3S1754和D22S683基因座在中國成都地區(qū)漢族群體中的遺傳多態(tài)性研究[J];法律與醫(yī)學(xué)雜志;1999年02期

3 侯一平;法醫(yī)常染色體STR分型[J];中國法醫(yī)學(xué)雜志;2001年01期

4 侯一平;第四講 法醫(yī)DNA分析的進(jìn)展[J];中國法醫(yī)學(xué)雜志;2001年04期

5 張勇,丁梅,王保捷,龐灝,劉清利,孫志剛;中國漢族、維吾爾族、哈薩克族D3S1744、D18S849基因座的遺傳多態(tài)性[J];中國法醫(yī)學(xué)雜志;2004年01期

6 邵敏杰;STR在產(chǎn)前診斷中的應(yīng)用[J];國外醫(yī)學(xué)(計(jì)劃生育分冊);2004年02期

7 聶勝潔;短串聯(lián)重復(fù)序列的研究及應(yīng)用[J];國外醫(yī)學(xué).遺傳學(xué)分冊;2002年05期

8 李大啟,王椿,秦尤文,張帆,謝匡成,顏式可,金力,趙壽元;異基因外周血干細(xì)胞移植后淋巴細(xì)胞和粒細(xì)胞嵌合體的動態(tài)改變[J];臨床血液學(xué)雜志;2005年02期

9 許業(yè)莉,蔡穎,相大鵬,梁希,揚(yáng)葉華;15個(gè)STR位點(diǎn)在潮汕地區(qū)人群的群體遺傳學(xué)分析[J];檢驗(yàn)檢疫科學(xué);2005年02期

10 豐慧根,范雪暉,李延蘭,申成斌,劉慧娟,林俊堂,王文鋒;D1S549等6個(gè)短串重復(fù)序列基因座在河南漢族群體的遺傳多態(tài)性[J];新鄉(xiāng)醫(yī)學(xué)院學(xué)報(bào);2003年05期

，

本文編號：2488681