【摘要】：為了解決法醫(yī)學(xué)人員對(duì)于嗜尸性蠅類的鑒定難題,對(duì)中山市、廣州市及西安市5種常見(jiàn)嗜尸性蠅類共17個(gè)樣本,對(duì)其線粒體COⅠ基因的348bp大小片段進(jìn)行了RFLP和DNA序列分析,采用ABI377測(cè)序儀測(cè)序,DNASTAR軟件預(yù)測(cè)限制性位點(diǎn),DdeⅠ,DraⅠ和HinfⅠ3種限制性內(nèi)切酶消化,非變性聚丙烯酰胺凝膠電泳檢測(cè)酶切結(jié)果,MEGA3.0軟件包進(jìn)行序列分析和構(gòu)建系統(tǒng)發(fā)育樹(shù)。結(jié)果表明:采用mtDNA擴(kuò)增結(jié)合銀染技術(shù)和DNA序列分析,均可以方便快捷地進(jìn)行上述三地5種常見(jiàn)嗜尸性蠅類的種類鑒定。本文結(jié)果為法醫(yī)昆蟲(chóng)學(xué)中嗜尸性昆蟲(chóng)DNA鑒定數(shù)據(jù)庫(kù)的建立提供了數(shù)據(jù)資料。
[Abstract]:In order to solve the difficult problem of forensic personnel in the identification of cadaveric flies, 17 common cadaveric flies were collected from Zhongshan, Guangzhou and Xi'an, and the 348bp fragments of CO 鈪，

本文編號(hào)：2488076