發(fā)布時(shí)間：2019-01-23 07:57

【摘要】：目的:觀察大鼠皮膚切創(chuàng)愈合過程中誘導(dǎo)型一氧化氮合酶(inducible nitric oxide synthases, iNOS)和內(nèi)皮型一氧化氮合酶(endothelial nitric oxide synthases, eNOS)蛋白表達(dá)的變化,以探討其在法醫(yī)實(shí)踐中用于推斷損傷時(shí)間的可能性。 方法:建立大鼠皮膚切創(chuàng)傷模型,應(yīng)用免疫組織化學(xué)技術(shù)(SP 法)和圖像分析方法,研究不同損傷時(shí)間(生前傷1h-14d) 大鼠皮膚創(chuàng)緣組織中iNOS 和eNOS 蛋白表達(dá)的變化及死后不同時(shí)間對iNOS 和eNOS 蛋白的影響。 結(jié)果:(1)生前傷不同時(shí)間iNOS 蛋白表達(dá)的變化:各時(shí)間點(diǎn)創(chuàng)口對側(cè)組織未見iNOS 表達(dá)。大鼠皮膚切創(chuàng)傷后角朊細(xì)胞、汗腺、毛囊、和骨骼肌細(xì)胞及創(chuàng)傷后肉芽組織的炎細(xì)胞、成纖維細(xì)胞、血管內(nèi)皮細(xì)胞均不同程度的表達(dá)iNOS 蛋白,免疫組化染色iNOS 蛋白呈棕黃色顆粒樣分布于上述細(xì)胞胞漿。①肉芽組織: 創(chuàng)傷后6h,創(chuàng)緣附近浸潤的巨噬細(xì)胞、中性粒細(xì)胞開始表達(dá)iNOS 蛋白,呈弱陽性染色;12h,陽性染色的巨噬細(xì)胞和中性粒細(xì)胞增多;創(chuàng)傷后1d,iNOS 蛋白表達(dá)量達(dá)到高峰,并以較高水平維持至傷后5d, 可見到強(qiáng)陽性顆粒分布于炎細(xì)胞、成纖維細(xì)胞、血管內(nèi)皮細(xì)胞胞漿;7d-14d,仍可見到成纖維細(xì)胞、纖維細(xì)胞、新生毛細(xì)血管內(nèi)皮細(xì)胞表達(dá)iNOS 蛋白,但陽性細(xì)胞數(shù)目逐漸減少,且染色程度逐漸減弱。②表皮:創(chuàng)傷后1h, 創(chuàng)緣角朊細(xì)胞表達(dá)微量
[Abstract]:Objective: to observe the expression of inducible nitric oxide synthase (inducible nitric oxide synthases, iNOS) and endothelial nitric oxide synthase (endothelial nitric oxide synthases, eNOS) protein in the healing process of rat skin incision. To explore the possibility of its use in forensic practice to infer the time of injury. Methods: the rat skin injury model was established. Immunohistochemical technique (SP) and image analysis were used. To study the changes of iNOS and eNOS protein expression in the injured skin tissue of rats with different injury time (1h-14d) and the effect of different postmortem time on iNOS and eNOS protein. Results: (1) the changes of iNOS protein expression at different time points: no iNOS expression was found in the contralateral tissue of wound at different time points. INOS protein was expressed in posttraumatic keratinocytes, sweat glands, hair follicles, inflammatory cells of skeletal muscle cells and posttraumatic granulation tissue, fibroblasts and vascular endothelial cells in rats. Immunohistochemical staining showed that iNOS protein distributed in the cytoplasm of the above cells as brown granules. 1 granulation tissue: at 6 hours after trauma, macrophages infiltrated near the wound edge, neutrophils began to express iNOS protein and showed weak positive staining. At 12 h, the number of positive staining macrophages and neutrophils increased. The expression of iNOS reached its peak on the 1st day after trauma, and maintained at a high level until 5 days after injury. The strong positive granules were found in the cytoplasm of inflammatory cells, fibroblasts and vascular endothelial cells. At 7 d to 14 d, fibroblasts could still be seen. INOS protein was expressed in neocapillary endothelial cells, but the number of positive cells gradually decreased and the staining degree gradually weakened. 2 epidermis: 1 hour after trauma, microamounts of keratinocytes expressed in wound edge keratinocytes
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：D919

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