【摘要】：前言 皮膚損傷愈合的過程可分為三個階段,即炎癥期、增生期及組織重建期。中性粒細胞和巨噬細胞的滲出是該過程重要的防御機制,它們吞噬并消化細菌等外源性微粒狀物質(zhì)及死亡細胞碎片。許多研究表明在這些細胞數(shù)量減少的同時有細胞的凋亡。 細胞的程序性死亡也叫做細胞凋亡,是細胞自我毀壞的生理過程。凋亡在生物發(fā)育、維持多細胞生物體內(nèi)環(huán)境穩(wěn)定及宿主防御中起十分重要的作用。細胞凋亡的失調(diào)涉及癌癥、自身免疫疾病、神經(jīng)變性疾病及局部缺血損傷等多種疾病。 caspase是誘導(dǎo)細胞凋亡的重要的蛋白酶,有14個家族成員:caspase-1～-14。caspase既是半胱氨酸蛋白酶能以半胱氨酸為親核基團裂解底物,又是天冬氨酸蛋白酶可將天冬氨酸C-端肽鏈酶切。caspase家族的反應(yīng)是級聯(lián)放大的。上游啟動型caspase(如caspase-8、-9)被caspase適配器活化后將激活效應(yīng)型caspase(如caspase-3、-6、-7)使細胞蛋白裂解,導(dǎo)致細胞凋亡。啟動型caspase的活化是細胞凋亡的調(diào)控點。 作為caspase家族的啟動因子,caspase-8在皮膚損傷愈合過程中起重要作用。本研究用免疫組織化學(xué)染色技術(shù)和Western blot方法檢測小鼠皮膚切創(chuàng)愈合過程中caspase-8在多核粒細胞、單核細胞及成纖維細胞中的表達并探討通過檢測caspase-8表達的規(guī)律推測皮膚損傷時間的可行性。 材料和方法 1.動物模型建立及分組 健康成年清潔級昆明系雄性小鼠33只,體重35g-40g,隨機分成11
[Abstract]:The healing process of skin injury can be divided into three stages: inflammation stage, proliferative stage and tissue reconstruction stage. The exudation of neutrophils and macrophages is an important defense mechanism in this process. They engulf and digest foreign particulate matter such as bacteria and death cell fragments. Many studies have shown that the number of these cells decreases at the same time as cell apoptosis. Programmed cell death, also known as apoptosis, is a physiological process of cell self-destruction. Apoptosis plays an important role in biological development, maintenance of environmental stability in multicellular organisms and host defense. Apoptosis disorders are associated with cancer, autoimmune diseases, neurodegenerative diseases and ischemic damage. Caspase is an important protease that induces cell apoptosis. There are 14 family members: caspase-1~-14.caspase is a cysteine protease that can use cysteine as the nucleophilic group lysate substrate. Aspartate protease can cut the C-terminal peptide chain of aspartic acid. The reaction of caspase family is amplified by cascade. When upstream caspase (such as caspase-8,-9) is activated by caspase adaptor, the activated caspase (such as caspase-3,-6,-7) cleavage the cell protein and induce cell apoptosis. Activation of priming caspase is the regulatory point of apoptosis. As the promoter of caspase family, caspase-8 plays an important role in the healing of skin injury. In this study, immunohistochemical staining and Western blot methods were used to detect caspase-8 in polymorphonuclear granulocytes (PMN) during skin wound healing in mice. The expression of caspase-8 in monocytes and fibroblasts and the feasibility of estimating the time of skin injury by detecting the expression of caspase-8. Materials and methods 1. Establishment and grouping of healthy adult Kunming male mice of clean grade, weighing 35g-40g, randomly divided into 11
【學(xué)位授予單位】：中國醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：D919

【參考文獻】

相關(guān)期刊論文 前3條

1 杜宇,官大威,趙銳;小鼠皮膚切創(chuàng)愈合過程中caspase-6、-7表達的免疫組織化學(xué)研究[J];中國法醫(yī)學(xué)雜志;2003年05期

2 張蔚,楊文武,劉元姣,洛若愚;宮頸癌組織中Fas、FasL及caspase-8表達及其臨床意義[J];實用癌癥雜志;2003年03期

3 鄧志鋒,李明,汪泱,宋書欣;腦缺血預(yù)處理后Caspase-8蛋白的表達與神經(jīng)元的保護作用[J];中國臨床康復(fù);2004年07期

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本文編號：2322628