死后人玻璃體液化學(xué)成分規(guī)律性改變對(duì)推斷死亡時(shí)間的法醫(yī)學(xué)研究
發(fā)布時(shí)間：2018-10-30 11:26
【摘要】： 背景: 死亡時(shí)間(Postmortem Interval PMI)是法醫(yī)學(xué)尸體檢驗(yàn)要求解決的重要問(wèn)題之一,對(duì)進(jìn)一步確定案件性質(zhì)和闡明作案過(guò)程有重要的價(jià)值。 當(dāng)前推斷死亡時(shí)間的方法主要包括:(1)根據(jù)尸體現(xiàn)象推斷死亡時(shí)間。(2)根據(jù)胃腸內(nèi)容物消化程度推斷死亡時(shí)間。(3)利用昆蟲(chóng)學(xué)知識(shí)推斷死亡時(shí)間。(4)根據(jù)DNA降解程度推斷死亡時(shí)間。(5)根據(jù)酶化學(xué)和組織學(xué)變化推斷死亡時(shí)間等等。 由于人眼玻璃體本身具有受到眼眶的保護(hù)及相對(duì)隔絕的解剖特點(diǎn),尸體腐敗和外界污染等因素對(duì)其影響小。玻璃體液容易獲取,檢測(cè)方法簡(jiǎn)便、相對(duì)可靠,尤其是死后玻璃體液中很多物質(zhì)的化學(xué)變化較血液與腦脊液內(nèi)的要緩慢得多,且相對(duì)穩(wěn)定,變化規(guī)律性較強(qiáng)。根據(jù)文獻(xiàn)報(bào)道,目前大多數(shù)對(duì)眼玻璃體液的研究還停留在動(dòng)物實(shí)驗(yàn)階段。由于人和動(dòng)物的種屬差異性,很多的研究結(jié)果在法醫(yī)實(shí)踐中價(jià)值不大。本研究用死后人的玻璃體液,通過(guò)先進(jìn)的儀器和有效的方法,檢測(cè)到死后玻璃體液更多的指標(biāo),發(fā)現(xiàn)更多與死亡時(shí)間密切相關(guān)的成分。 目的: 用玻璃體液化學(xué)成分規(guī)律變化推斷死亡時(shí)間,對(duì)多指標(biāo)進(jìn)行分析,建立各自的回歸方程以及多元回歸方程,并計(jì)算出相應(yīng)的死亡時(shí)間的預(yù)測(cè)區(qū)間,綜合分析,減少誤差,使玻璃體液能更廣泛地應(yīng)用于法醫(yī)實(shí)踐,盡可能更加準(zhǔn)確地推測(cè)死亡時(shí)間。 方法: 1.用日本產(chǎn)HITACHI.7060全自動(dòng)生化分析儀對(duì)玻璃體液化學(xué)成分(鈉、氯、尿素、肌酐、鉀、鈣、鎂、無(wú)機(jī)磷)進(jìn)行測(cè)定。 2.人體死后不同時(shí)間玻璃體液化學(xué)成分測(cè)定的樣本:死亡時(shí)間在0.3～148小時(shí)以?xún)?nèi)尸體的雙眼玻璃體液。 3.用SPSS13.0統(tǒng)計(jì)軟件,將所得數(shù)據(jù)進(jìn)行正態(tài)性檢驗(yàn)后再進(jìn)行單元及多元回歸分析。 結(jié)果: 1.玻璃體液中鈉、氯、尿素、肌酐、鉀、鈣、鎂、無(wú)機(jī)磷與死亡時(shí)間均有一定的相關(guān)性。 2.鉀離子濃度與死亡時(shí)間線性關(guān)系明顯,方程為PMI=0.702+0.186K~+(r=0.512;R~2=0.262,校正R~2=0.259),PMI的95%預(yù)測(cè)區(qū)間為:(PMI-1.96×3.60,PMI+1.96×3.60)。玻璃體液中無(wú)機(jī)磷濃度與死亡時(shí)間的相關(guān)性大,隨死亡時(shí)間緩慢地升高,方程為PMI=1.617+0.681P(r=0.544;R~2=0.296,校正R~2=0.293),PMI的95%預(yù)測(cè)區(qū)間為:(PMI—1.96×0.85,PMI+1.96×0.85)。 3.鉀離子(K~+)、鈉離子(Na~+)、磷(P)、鎂離子(Mg~(2+))與死亡時(shí)間的多元回歸方程為:PMI=5.292+0.142K~+-0.022Na~++0.670P-1.007Mg~(2+)(r=0.633;R~2=0.4000,校正R~2=0.388),PMI的預(yù)測(cè)區(qū)間為:(PMI-1.96×1.31.PMI+1.96×1.31)。 結(jié)論: 1、玻璃體液中的鈉、氯離子與死亡時(shí)間呈負(fù)相關(guān);尿素氮、肌酐、鉀離子、鈣離子、鎂離子、無(wú)機(jī)磷與死亡時(shí)間呈正相關(guān);其中無(wú)機(jī)磷和鉀離子濃度與死亡時(shí)間線性關(guān)系最明顯。 2、結(jié)合磷、鎂離子、鉀離子和鈉離子一起建立多元回歸方程,可以加強(qiáng)方程的擬合度,更好的反應(yīng)出玻璃體液隨死亡時(shí)間變化的關(guān)系。 3、本研究未發(fā)現(xiàn)左、右眼玻璃體液各化學(xué)成分有差異。
[Abstract]:Background: Postmorte Interval PMI (PMI) is one of the important issues to be resolved in forensic autopsy. It is important to further determine the nature of the case and clarify the course of the crime. The method to infer the time of death mainly includes: (1) According to the corpse Body phenomenon inferred the time of death. (2) Treatment based on gastrointestinal contents Extrapolation of death time. (3) Use of insects Learn to infer the time of death. (4) According to the DNA drop The time of death can be inferred. (5) According to the chemical and histological changes of the enzyme due to the fact that the vitreous body of the human eye has the characteristics of being protected by the orbit and relative isolation, the body rot The influence of the factors such as defeat and external pollution is small. The vitreous humor is easy to acquire, the detection method is simple, relatively reliable, especially after the death, the chemical changes of many substances in the vitreous humor are much slower than that in the cerebrospinal fluid. and is relatively stable and has strong change regularity, The study of liquid also remains in the animal experiment stage. Due to the difference of species and species in humans and animals, a lot of The results of this study are of great value in forensic practice. The study uses the glass body fluid of the deceased to detect the more indexes of vitreous humor after death through advanced instruments and effective methods, and finds that More vs. Objective: To estimate the dead time with the change of chemical composition of vitreous humor, to analyze the multi-index, to establish the regression equation and multiple regression equation, and to calculate the death time. the prediction interval of the corresponding death time is calculated, the comprehensive analysis is carried out, the error is reduced, and the body fluid of the glass can be wider. pan-to-ground For forensic practice, estimate the time of death more accurately, as far as possible. Method: 1. Use the Japanese HITACHI. 7060 full-automatic biochemical analyzer Determination of chemical components of vitreous humor (sodium, chlorine, urea, potassium, potassium, calcium, magnesium, and inorganic phosphorus). A sample of the chemical composition of vitreous humor: the time of death is within 0. 3-148 hours of the body's eyes vitreous humor. 3. Using SPSS13. 0 statistical software, the obtained data is subjected to positive state inspection, and then the unit and the multiple regression are performed. Results: 1. There was a certain correlation between sodium, chlorine, urea, potassium bicarbonate, potassium, calcium, magnesium, inorganic phosphorus and death time in vitreous humor. The linear relation between potassium ion concentration and death time was obviously linear, and the equation was PMI = 0.702 + 0.186K ~ + (r = 0.512; R ~ 2 = 0). 262. The 95% prediction interval of PMI (R ~ 2 = 0. 259) was: (PMI-1.96, 3.60, PMI + 1.96, 3.60). The correlation between inorganic phosphorus concentration and death time in vitreous humor increased slowly with death time. The equation was PMI = 1.617 + 0.681P (r = 0.544; R ~ 2 = 0). 296. The 95% prediction interval of PMI (R ~ 2 = 0. 293) was: (PMI = 1. 96, 0. 85, PMI + 1. 96, 0. 85). 3. The multiple regression equation of potassium ion (K +), sodium ion (Na ~ +), phosphorus (P), magnesium ion (Mg ~ (2 +)) and death time was: PMI = 5.292 + 0.142K ~ +-0.022Na ~ + 0.0670P-1.007Mg ~ (2 +) (r = 0.0633). ;R~ The prediction interval of PMI was: (PMI-1.96-1.31. PMI + 1.96-1.31). Conclusion: 1. Glass There was a negative correlation between sodium, chloride ion and death time in body fluids; potassium ions, potassium ions, potassium ions, calcium ions, magnesium ions and inorganic phosphorus were positively correlated with the time of death; in which inorganic phosphorus and potassium ions the linear relation between the concentration and the death time is most obvious.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類(lèi)號(hào)】：D919
本文編號(hào)：2299868
[Abstract]:Background: Postmorte Interval PMI (PMI) is one of the important issues to be resolved in forensic autopsy. It is important to further determine the nature of the case and clarify the course of the crime. The method to infer the time of death mainly includes: (1) According to the corpse Body phenomenon inferred the time of death. (2) Treatment based on gastrointestinal contents Extrapolation of death time. (3) Use of insects Learn to infer the time of death. (4) According to the DNA drop The time of death can be inferred. (5) According to the chemical and histological changes of the enzyme due to the fact that the vitreous body of the human eye has the characteristics of being protected by the orbit and relative isolation, the body rot The influence of the factors such as defeat and external pollution is small. The vitreous humor is easy to acquire, the detection method is simple, relatively reliable, especially after the death, the chemical changes of many substances in the vitreous humor are much slower than that in the cerebrospinal fluid. and is relatively stable and has strong change regularity, The study of liquid also remains in the animal experiment stage. Due to the difference of species and species in humans and animals, a lot of The results of this study are of great value in forensic practice. The study uses the glass body fluid of the deceased to detect the more indexes of vitreous humor after death through advanced instruments and effective methods, and finds that More vs. Objective: To estimate the dead time with the change of chemical composition of vitreous humor, to analyze the multi-index, to establish the regression equation and multiple regression equation, and to calculate the death time. the prediction interval of the corresponding death time is calculated, the comprehensive analysis is carried out, the error is reduced, and the body fluid of the glass can be wider. pan-to-ground For forensic practice, estimate the time of death more accurately, as far as possible. Method: 1. Use the Japanese HITACHI. 7060 full-automatic biochemical analyzer Determination of chemical components of vitreous humor (sodium, chlorine, urea, potassium, potassium, calcium, magnesium, and inorganic phosphorus). A sample of the chemical composition of vitreous humor: the time of death is within 0. 3-148 hours of the body's eyes vitreous humor. 3. Using SPSS13. 0 statistical software, the obtained data is subjected to positive state inspection, and then the unit and the multiple regression are performed. Results: 1. There was a certain correlation between sodium, chlorine, urea, potassium bicarbonate, potassium, calcium, magnesium, inorganic phosphorus and death time in vitreous humor. The linear relation between potassium ion concentration and death time was obviously linear, and the equation was PMI = 0.702 + 0.186K ~ + (r = 0.512; R ~ 2 = 0). 262. The 95% prediction interval of PMI (R ~ 2 = 0. 259) was: (PMI-1.96, 3.60, PMI + 1.96, 3.60). The correlation between inorganic phosphorus concentration and death time in vitreous humor increased slowly with death time. The equation was PMI = 1.617 + 0.681P (r = 0.544; R ~ 2 = 0). 296. The 95% prediction interval of PMI (R ~ 2 = 0. 293) was: (PMI = 1. 96, 0. 85, PMI + 1. 96, 0. 85). 3. The multiple regression equation of potassium ion (K +), sodium ion (Na ~ +), phosphorus (P), magnesium ion (Mg ~ (2 +)) and death time was: PMI = 5.292 + 0.142K ~ +-0.022Na ~ + 0.0670P-1.007Mg ~ (2 +) (r = 0.0633). ;R~ The prediction interval of PMI was: (PMI-1.96-1.31. PMI + 1.96-1.31). Conclusion: 1. Glass There was a negative correlation between sodium, chloride ion and death time in body fluids; potassium ions, potassium ions, potassium ions, calcium ions, magnesium ions and inorganic phosphorus were positively correlated with the time of death; in which inorganic phosphorus and potassium ions the linear relation between the concentration and the death time is most obvious.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2008
【分類(lèi)號(hào)】：D919
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