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大鼠死后肺臟FTIR光譜變化與死亡時(shí)間的關(guān)系

發(fā)布時(shí)間:2018-10-21 11:56
【摘要】:應(yīng)用傅里葉變換紅外(FTIR)光譜分析大鼠死后肺臟組織隨死亡時(shí)間推移的化學(xué)降解過程,為法醫(yī)死亡時(shí)間推斷提供新的研究方法。結(jié)果表明,隨著死亡時(shí)間的推移,大鼠肺臟組織FTIR光譜的主要吸收峰峰位沒有明顯變化,而其峰高有明顯差異:(1)與核酸有關(guān)的1080,1241cm-1譜帶的峰強(qiáng)呈下降趨勢(shì)。(2)酰胺Ⅰ(I1647),Ⅱ(I1541)峰強(qiáng)比隨死亡時(shí)間推移下降。1338和1313cm-1處的吸收峰強(qiáng)略有升高。(3)1460,1400,1170cm-1的譜帶沒有明顯變化趨勢(shì)。(4)死亡72h后,1120cm-1處出現(xiàn)新的吸收峰,吸收強(qiáng)度隨時(shí)間推移逐漸增高;這一結(jié)果表明FTIR光譜分析技術(shù)有望成為法醫(yī)死亡時(shí)間推斷的有效方法。
[Abstract]:Fourier transform infrared (FTIR) spectroscopy was used to analyze the chemical degradation process of rat lung tissues after death, which provided a new method for estimating the death time of forensic doctors. The results showed that there was no significant change in the peak position of the main absorption peaks in the FTIR spectra of the lung tissues of the rats with the passage of the death time. (1) the peak intensity of the nucleic acid-related band of 1080,1241cm-1 decreased. (2) the ratio of peak strength of amide 鈪,

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