【摘要】：研究背景及目的:毒鼠強(qiáng)是一種劇毒鼠藥,對人、畜危害極大,臨床診治和法醫(yī)學(xué)鑒定經(jīng)常遇到毒鼠強(qiáng)中毒的案例,但是對毒鼠強(qiáng)中毒的機(jī)制仍不明確。GABA是中樞神經(jīng)系統(tǒng)主要的抑制性神經(jīng)遞質(zhì);GABA_AR是GABA的受體之一,在腦內(nèi)分布最普遍,α1亞單位與其功能密切相關(guān)。GABA及GABA_AR在癲癇、顱腦損傷、神經(jīng)毒劑中毒等疾病、損傷和中毒模型中的表達(dá)和/或結(jié)合都有改變,但在毒鼠強(qiáng)中毒中的表達(dá)情況尚未見報道。本研究對毒鼠強(qiáng)中毒大鼠腦內(nèi)GABA及GABA_AR-α1的表達(dá)情況和變化規(guī)律進(jìn)行研究,以探討GABA及GABA_AR與毒鼠強(qiáng)中毒的關(guān)系,為研究毒鼠強(qiáng)中毒機(jī)制提供實驗依據(jù),并為法醫(yī)學(xué)上毒鼠強(qiáng)中毒鑒定提供可能的新途徑。 方法:健康Sprague-Dawley大鼠(S-D大鼠)60只,隨機(jī)分為正常對照組、空白對照組、急性中毒組和慢性中毒組,其中慢性中毒組按中毒后處死時間不同分成30min,3h,6h,12h,24h,3d,5d,7d,10d組,每組5只。急性中毒組每只以1.0mg/kg的劑量用毒鼠強(qiáng)懸濁液灌胃,慢性中毒組每只以0.1mg/kg的劑量用毒鼠強(qiáng)溶液灌胃制作中毒模型。應(yīng)用免疫組化技術(shù)和圖像分析儀對GABA及GABA_AR-α1的表達(dá)情況和變化規(guī)律進(jìn)行研究。 結(jié)果:①正常大鼠腦組織內(nèi)GABA及GABA_AN-α1表達(dá)較為廣泛,維持在中等水平;②急性中毒組腦內(nèi)GABA及GABA_AR-α1表達(dá)均下降;
[Abstract]:Background and objective: tetramine is a highly toxic rodenticide, which is harmful to human and animal. The cases of tetramine poisoning are often encountered in clinical diagnosis, treatment and forensic medicine. However, the mechanism of tetramine poisoning is still unclear. GABA is one of the main inhibitory neurotransmitters in the central nervous system. GABAAR is one of the receptors of GABA, and the distribution of 偽 1 subunit is most common in the brain. The 偽 1 subunit is closely related to its function in epilepsy and brain injury. The expression and / or combination of nerve agent poisoning and other diseases, injury and poisoning models have been changed, but the expression in tetramine poisoning has not been reported. In this study, we studied the expression and changes of GABA and GABA AR- 偽 1 in the brain of rats with tetramine poisoning, in order to explore the relationship between GABA and GABA_AR and tetramine poisoning, and to provide experimental basis for studying the mechanism of tetramine poisoning. It also provides a new way for the identification of tetramine poisoning in forensic medicine. Methods: sixty healthy Sprague-Dawley rats (S-D rats) were randomly divided into normal control group, blank control group, acute poisoning group and chronic poisoning group. According to the time of death, chronic poisoning group was divided into three groups: 30min, 3h, 12h, 24h, 3h, 3d, 5d, 7d, 10d group. The acute poisoning group was given the dose of 1.0mg/kg and the chronic poisoning group was fed with tetramine suspension. The chronic poisoning group was given the dose of 0.1mg/kg to make the poisoning model. Immunohistochemical technique and image analyzer were used to study the expression and changes of GABA and GABAAR- 偽 1. Results the expression of GABA and GABAAR- 偽 1 in brain tissue of normal rats was more extensive, and the expression of GABA and GABAAR- 偽 1 in the brain of acute poisoning group was decreased.
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：D919

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