發(fā)布時間：2018-06-13 14:53

  本文選題：死亡時間(PMI) + 熒光半定量技術(shù)��； 參考：《中國現(xiàn)代醫(yī)學雜志》2006年11期

【摘要】：目的探討熒光半定量逆轉(zhuǎn)錄-聚合酶鏈式反應(fluorescencesemi-quantitativereversetranscrip-tase-polymerasechainreaction,RT-PCR)技術(shù)檢測大鼠死后不同時間腎3-磷酸甘油醛脫氫酶(GAPDH)mRNA含量變化對死亡時間(postmorteminterval,PMI)推斷的應用價值。方法28只大鼠斷頸處死后置于20℃溫控系統(tǒng)內(nèi),利用熒光標記半定量RT-PCR技術(shù)檢測大鼠腎GAPDHmRNA在死后48h內(nèi)相對表達量的變化情況。結(jié)果在死后即刻至40h內(nèi)大鼠腎均可檢測出GAPDHmRNA,且其擴增產(chǎn)物呈規(guī)律性下降。結(jié)論熒光半定量RT-PCR技術(shù)檢測大鼠死后腎GAPDHmRNA含量變化對PMI推斷具有一定的應用價值。
[Abstract]:Objective to investigate the value of fluorescence semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) in determining the content of GAPDHN mRNA in the kidney of postmortem intervalPMIs in postmortem intervalPMIs of rats at different postmortem time after death by using fluorescence semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Methods 28 rats were killed by cervical amputation and placed in the temperature control system at 20 鈩，

本文編號：2014396