本文選題：利多卡因 + 死后再分布��； 參考：《山西醫(yī)科大學》2005年碩士論文

【摘要】：目的 (1)建立犬的利多卡因靜脈注射、蛛網(wǎng)膜下腔麻醉、硬膜外麻醉致死的死后再分布動物模型; (2)比較三種死后再分布模型致死犬常溫下不同時間點利多卡因在心血、周圍血、肝臟和腦中的分布規(guī)律,為利多卡因硬膜外麻醉意外死亡和中毒案件的法醫(yī)學鑒定提供科學依據(jù); (3)比較三種死后再分布模型致死犬在常溫下不同時間點心血、周圍血、肝臟和腦中利多卡因的分布特點, 探討死后再分布機制。 方法 1 動物模型每種模型各用犬9 只,隨機分為實驗組(6 只)和對照組(3 只)兩組,實驗組犬5 分鐘內(nèi)分別經(jīng)股靜脈、蛛網(wǎng)膜下腔、硬膜外腔勻速注入利多卡因5×15mg/kg,對照組分別在對應部位注入等體積生理鹽水。 2 生命體征記錄方法BL-生物機能實驗系統(tǒng)全程記錄死后再分布模型從開始給藥到動物死亡的心電、血壓和呼吸等主要生命體征的變化。 3 樣品采集與處理 心電、血壓和呼吸全部消失后,置于室溫下,分別于死后0,1,4,8,24,48,72 小時取心血、周圍血、肝臟、大腦,檢測實驗組犬上述檢材中利多卡因含量,對照組犬各對應檢材用作空白; 樣品經(jīng)酸、堿化處理后,乙醚萃取,根據(jù)利多卡因的特征離子峰、保留時間氣相色譜-質(zhì)譜聯(lián)用定性,內(nèi)標法、工作曲線法氣相色譜定量。 結果 ( 1 ) 癥狀靜脈注射實驗組動物開始給藥后出現(xiàn)瞳孔縮小、呼吸困難、口吐粘稠狀白色泡沫、角弓反張、大小便失禁。蛛網(wǎng)膜下腔麻醉、硬膜外腔麻醉實驗組動物出現(xiàn)癥狀與上述相似。三種模型對照組均未出現(xiàn)以上癥狀,未出現(xiàn)死亡。
[Abstract]:Purpose 1) to establish the animal model of lidocaine intravenous injection, subarachnoid anesthesia and epidural anesthesia. (2) to compare the distribution of lidocaine in heart blood, peripheral blood, liver and brain at different time points at room temperature in three kinds of postmortem redistributive models. To provide scientific basis for forensic identification of lidocaine epidural anesthesia accidental death and poisoning cases. (3) to compare the distribution characteristics of lidocaine in heart blood, peripheral blood, liver and brain of three kinds of postmortem redistributive model dogs at different time points under normal temperature, and to explore the mechanism of postmortem redistribution. Method 1Nine dogs with each animal model were randomly divided into two groups: the experimental group (n = 6) and the control group (n = 3). The dogs in the experimental group were treated by femoral vein and subarachnoid cavity within 5 minutes. Lidocaine was injected into the epidural cavity at a uniform rate of 5 脳 15 mg / kg, and the control group was injected with saline of the same volume in the corresponding parts. 2 vital signs recording method BL-Biological function Experimental system recorded the changes of the main vital signs from the beginning of administration to the death of animals, blood pressure and respiration in the whole course of the model of postmortem redistribution. 3 sampling and processing After the disappearance of ECG, blood pressure and respiration, they were placed at room temperature. The blood samples, peripheral blood, liver and brain were collected for 72 hours after death. The lidocaine content in the above samples was detected in the experimental group, and the corresponding samples in the control group were used as blank. The samples were extracted by ether after acid and alkali treatment. The retention time was determined by gas chromatography-mass spectrometry (GC-MS) according to the characteristic ion peak of lidocaine. Result The main results were as follows: (1) the symptoms of the experimental group were pupillary dilatation, dyspnea, sticky white foam, angle arch reversion, incontinence. The symptoms of subarachnoid anesthesia and epidural anesthesia in experimental group were similar to those mentioned above. The above symptoms and death were not found in the three model control groups.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2005
【分類號】：D919

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