發(fā)布時(shí)間：2018-05-17 14:31

  本文選題：變性高效液相色譜(DHPLC) + 線粒體DNA(mtDNA)��； 參考：《四川大學(xué)》2004年博士論文

【摘要】：目的 建立新的mtDNA多態(tài)遺傳標(biāo)記的變性高效液相色譜檢測技術(shù),為法醫(yī)學(xué)疑難檢材的檢測提供新的技術(shù)手段;利用變性高效液相色譜技術(shù),尋找適合作為法醫(yī)遺傳標(biāo)記的mtDNA編碼區(qū)多態(tài)遺傳標(biāo)記,以期提高mtDNA在法醫(yī)學(xué)檢測中的應(yīng)用價(jià)值。方法 應(yīng)用primer3引物設(shè)計(jì)軟件,對mtDNA控制區(qū)和編碼區(qū)分別設(shè)計(jì)引物,用以進(jìn)行PCR擴(kuò)增。通過構(gòu)建DNA池及樣本間兩兩比較的方法,確定DHPLC發(fā)現(xiàn)異源雙鏈的效能,從而建立mtDNA多態(tài)分析的DHPLC方法。用建立的方法,在成都漢族群體120名無關(guān)個(gè)體中,對mtDNA編碼區(qū)各基因座進(jìn)行多態(tài)性研究,獲得等位基因及單倍型頻率、變異度及標(biāo)準(zhǔn)誤,并進(jìn)行種屬特異性、組織同一性以及陳舊骨骼、毛干等法醫(yī)學(xué)疑難檢材的DHPLC分析。結(jié)果 應(yīng)用DHPLC技術(shù),對兩個(gè)mtDNA控制區(qū)基因座的擴(kuò)增產(chǎn)物成功進(jìn)行了異源雙鏈分析,建立了適合法醫(yī)學(xué)快速檢測的DHPLC方法。針對mtDNA編碼區(qū)總計(jì)1435bp范圍,成功建立了DHPLC分析方法。根據(jù)我們設(shè)計(jì)的分析片段的位置,把此mtDNA編碼區(qū)定義為7個(gè)基因座,分別稱為C、D、E、F、G、H和I基因座。對七個(gè)mtDNA編碼區(qū)基因座的多態(tài)性分析顯示,變
[Abstract]:Objective to establish a new denaturing high performance liquid chromatography (DHPLC) technique based on mtDNA polymorphic genetic markers to provide a new method for the detection of difficult forensic materials, and to use denatured high performance liquid chromatography (DHPLC). In order to improve the application value of mtDNA in forensic medicine detection, the polymorphic genetic markers of mtDNA coding region were found to be suitable for forensic genetic markers. Methods primer3 primer design software was used to design primers for mtDNA control region and coding region for PCR amplification. The effectiveness of DHPLC in discovering heterologous double chains was determined by constructing a pairwise comparison between DNA pool and sample, and the DHPLC method for mtDNA polymorphism analysis was established. By using the established method, the allele and haplotype frequency, variation and standard error were obtained in 120 unrelated individuals in Chengdu Han nationality population. The allele and haplotype frequency, variation and species-specificity were obtained by studying the polymorphism of each locus in the mtDNA coding region. Tissue identity and DHPLC analysis of old bones, dried hair and other difficult forensic materials. Results the amplification products of two mtDNA control region loci were successfully analyzed by DHPLC, and a DHPLC method suitable for forensic rapid detection was established. Aiming at the total 1435bp range of mtDNA coding region, a DHPLC analysis method is established successfully. According to the position of the analyzed fragment, this mtDNA coding region is defined as seven loci, which are called Candruff FGG H and I loci, respectively. Polymorphic analysis of seven mtDNA loci showed that
【學(xué)位授予單位】：四川大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2004
【分類號】：D919

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 侯一平,張思仲;溫度調(diào)控高效液相色譜探索人類基因組變異的進(jìn)展[J];中華醫(yī)學(xué)遺傳學(xué)雜志;2000年03期

2 廖林川,孟海英,侯一平,張思仲,顏有儀,蘇智廣,李英碧,吳瑾,張霽;用溫度調(diào)控高效液相色譜探索基因組單核苷酸多態(tài)性的方法研究[J];中華醫(yī)學(xué)遺傳學(xué)雜志;2000年03期

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本文編號：1901742