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  本文選題：過敏性休克 + P物質(zhì)�。� 參考：《山西醫(yī)科大學》2007年碩士論文

【摘要】： 目的:觀察P物質(zhì)在過敏性休克家兔胃、小腸、圓小囊組織中隨時間變化的規(guī)律,探討其在過敏性休克中的作用,試圖為過敏性猝死的法醫(yī)學鑒定提供可靠的形態(tài)學依據(jù)。 方法:30只家兔隨機分為實驗組(24只)和對照組(6只)。實驗組采用多人混合血清致敏家兔,建立過敏性休克模型。休克不同時間段(猝死即刻、休克0.5h、休克1h、休克2h)提取家兔肺臟、胃、小腸、圓小囊。(1)冰凍切片,常規(guī)HE染色;(2)應(yīng)用免疫組化(IHC)SP法進行P物質(zhì)染色,BI-2000計算機圖像分析系統(tǒng)進行圖像分析,計算陽性積分光密度值(IOD);(3)應(yīng)用原位雜交(ISH)對P物質(zhì)進行染色,BI-2000計算機圖像分析系統(tǒng)進行圖像分析,計算IOD。 結(jié)果:(1)IHC結(jié)果顯示:與對照組相比,實驗組各小組胃、腸、圓小囊P物質(zhì)蛋白表達明顯增加,且隨著休克時間的變化逐漸減弱。統(tǒng)計學方差分析結(jié)果為:胃組織F=198.008,腸組織F=56.796,圓小囊組織F=67.231,全部P值小于0.001,均有統(tǒng)計學意義。SNK檢驗結(jié)果為:胃組織各組之間兩兩比較均有差別;腸組織除對照組與休克2h組之間無統(tǒng)計學意義外,其余各組兩兩比較均有差別;圓小囊除對照組與休克2h組之間無統(tǒng)計學意義外,其余各組兩兩比較均有差別。(2)ISH結(jié)果顯示:與對照組相比,實驗組各小組胃、腸、圓小囊P物質(zhì)mRNA表達明顯增加,且隨著休克時間的變化逐漸減弱。統(tǒng)計學方差分析結(jié)果為:胃組織F=67.506,腸組織F=72.117,圓小囊組織F=216.798,全部P值小于0.001,均有統(tǒng)計學意義。SNK檢驗結(jié)果為:胃組織除對照組與休克2h組之間無統(tǒng)計學意義外,其余各組之間兩兩比較均有差別;腸組織各組之間兩兩比較均有差別:圓小囊各組之間兩兩比較均有差別。 結(jié)論:過敏性休克時,家兔胃、小腸、圓小囊組織的P物質(zhì)蛋白表達和mRNA表達明顯增加,且隨著休克時間的變化逐漸減弱。P物質(zhì)可為過敏性休克的法醫(yī)學鑒定提供客觀的形態(tài)學依據(jù)和參考指標。
[Abstract]:Objective: to observe the changes of substance P in the stomach, small intestine and small capsule of anaphylactic shock rabbits, and to explore the role of substance P in anaphylactic shock in order to provide reliable morphological basis for forensic identification of sudden anaphylactic death. Methods 30 rabbits were randomly divided into experimental group (n = 24) and control group (n = 6). Anaphylactic shock model was established in the experimental group by sensitizing rabbits with mixed human serum. Frozen sections of rabbit lung, stomach, small intestine, circular vesicle were extracted from different periods of shock (instant of sudden death, 0.5 h of shock, 1 hour of shock, 2 hours of shock). Routine HE staining was used to carry out the image analysis of substance P staining with BI-2000 computer image analysis system. The positive integral optical density (IOD) was calculated by in situ hybridization (ISH). The computer image analysis system of substance P staining BI-2000 was used for image analysis and IOD was calculated. Results compared with the control group, the expression of substance P protein in stomach, intestine and capsule of the experimental group increased significantly, and gradually decreased with the change of shock time. The results of ANOVA were as follows: stomach tissue (FN) 198.008, intestinal tissue (FN) 56.796 (P < 0.001), small capsule tissue (FN) 67.231 (all P < 0.001). The results of SNK test were as follows: there were differences among gastric tissue groups. There was no significant difference in intestinal tissue between the control group and the 2h shock group, but there was no significant difference between the other groups except the control group and the 2h shock group. The results showed that compared with the control group, the expression of substance P mRNA in the stomach, intestine and small capsule of the experimental group was significantly increased, and gradually weakened with the change of shock time. The results of ANOVA were as follows: stomach tissue (67.506), intestinal tissue (FN) 72.117, round capsule (FN) 216.798 (P < 0.001). The results of SNK test showed that there was no significant difference between gastric tissue and control group and 2 h shock group. There were differences between the other groups, and between the intestinal tissue groups and the small sac groups. Conclusion: during anaphylactic shock, the expression of substance P protein and mRNA in the stomach, small intestine and small capsule of rabbits were significantly increased. With the change of shock time, substance P can provide objective morphological basis and reference index for forensic identification of anaphylactic shock.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：D919

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